Abstract
This research has demonstrated that GEMs can be introduced and maintained in environmental soils for at least 18 months and that the population dynamics can be affected by nutrient augmentation. For example, the presence of naphthalene can be used to enrich for P. fluorescens HK44 in soils and the lack of naphthalene causes the GEM population to decline. Furthermore, this study has provided evidence that bioluminescence from GEMs can be used as a tool for monitoring and controlling the bioremediation process. A GEM can bioluminesce and report conditions that are favorable for bioremediation. In the case where a GEM is present and not bioluminescing, unfavorable environmental or physiological conditions are revealed. If unfavorable conditions are detected, analytical and microbiological assays as well as localized treatments can be used to diagnose and correct the existing problem. This strategy can lead to rapid responses and eliminate the need for expensive microcosm studies that may or may not be amenable to scale-up.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Dzombak, D.A. and Luthy, R.G. (1984). Estimating adsorption of polycyclic aromatic hydrocarbons on soils. Soil Sci. 137:292–308.
Ford, C.Z., Sayler G.S., and Burlage, R.S. (1998). Appl. Microbiol. Biotechnol., (submitted).
Heitzer, A., Webb, O.F., Thonnard, J.E., and Sayler, G.S. (1992). Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium. Appl. Environ. Microbiol. 58:1839–1846.
King, J.M.H., DiGrazia, P.M., Applegate, B., Burlage, R., Sanseverino, J., Dunbar, P., Larimer, F., and Sayler, G.S. (1990). Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation. Science 249:778–781.
Meighen, E.A. (1991). Molecular biology of bacterial bioluminescence. Microbiol. Rev. 55:123–142.
Sambrook, J., Fritsch E.F., and Maniatis T. (1989). Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Sayer, P. (1997). Risk assessment for a recombinant biosensor, p. 269–279. In G.S. Sayler, J. Sanseverino, and K.L. Davis, (eds.), Biotechnology in the Sustainable Environment, Plenum Press, New York.
Sayler, G.S., Matrubutham, U., Palmer, R., and Kelly, C. (1995). Application of molecular biology to real time monitoring in bioremediation. Organization for Economic Co-operation and Development, Amsterdam Workshop, p. 323–334.
Stormo, K.E., and Crawford, L. (1992). Preparation of encapsulated microbial cells for environmental applications. Appl. Environ. Microbiol. 58:727–730.
Webb, O.F., Bienkowski, P.R., Matrubutham, U., Evans, F.A., Heitzer, A., and Sayler, G.S. (1997). Kinetics and response of a Pseudomonas fluorescens HK44 biosensor. Biotech. Bioeng. 54:491–502.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Sayler, G.S. et al. (1999). Field Application of a Genetically Engineered Microorganism for Polycyclic Aromatic Hydrocarbon Bioremediation Process Monitoring and Control. In: Fass, R., Flashner, Y., Reuveny, S. (eds) Novel Approaches for Bioremediation of Organic Pollution. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4749-5_24
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4749-5_24
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7153-3
Online ISBN: 978-1-4615-4749-5
eBook Packages: Springer Book Archive