Abstract
Encouraging clinical results in the PDT of human tumors have been obtained with Photofrin II™ (P2) in early lung, stomach, bladder and skin cancers1. However, the low specificity of P2 delivery to tumor tissues and the lack of light absorption of the mixture of porphyrins constituting P2 in the 650 – 800 nm range, where tissues maximally transmit precludes the use of PDT for large tumor masses. In the case of skin tumors, selectivity has been recently improved by the induction of excess protoporphyrin IX (PP) in tumor cells through topical application of 5-aminolevulinic acid (ALA), a precursor of heme biosynthesis2.
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References
B.W. Henderson and T.J. Dougherty, How does photodynamic therapy work ? Photochem. Photobiol., 55: 145 (1992).
J.C. Kennedy and R.H. Pottier, Endogenous protoporphyrin IX, a clinical useful photosensitizer for photodynamic therapy, J. Photochem. Photobiol. B: Biol., 14: 275 (1992).
E. Ben-Hur, Second generation photosensitizers for photodynamic therapy of cancer, in “Radiation Research: A Twentieth Century Perspective” W.C. Dewey, M. Edington, R.J.M. Fry, E.J. Hall and G.F. Whitmore (eds.), Radiation Research, Academic Press, San Diego, CA, USA, pp 664–669 (1992).
G. Jori, M. Geltramini, E. Reddi, B. Salvato, A. Ragnan, L. Ziron, L. Tornio and T. Zsanov, Evidence for a major role of plasma lipoproteins as hematoporphyrin carriers in vivo, Cancer Lett., 24: 291 (1984).
K. Berg, H.B. Steen, J.W. Winkelman and J. Moan, Synergistic effects of photoactivated tetra(4-sulfona-tophenyl)porphyne and nocodazole on microtubule assembly, accumulation of cells in mitosis and cell survival J. Photochem. Photobiol., 13: 59 (1992).
R. Santus, P. Morliére and E. Kohen, The photobiology of the living cell as studied by microspec-trofluorometric techniques Photochem. Photobiol., 54: 1071 (1991).
J.M. Gaullier, M. Gèze, R. Santus, T. Sa e Melo, J.C. Mazière, M. Bazin, P. Morlière and L. Dubertret, Subcellular targets of ALA-induced endogenous porphyrins in cultured human keratinocytes and fibroblasts: a microspectrofluorometric study, Photochem. Photobiol., 62: 114 (1995).
M. Gèze, P. Morlière, J.C. Mazière, K.M. Smith and R. Santus. Lysosomes, a key target of hydrophobic photosensitizers proposed for photochemotherapeutic applications, J. Photochem. Photobiol. B: Biol., 20 (1993) 23–25.
P. Grellier, V. Agmon, P. Palud, J.C. Mazière, D. Rigommier, A. Dagan, R. Santus, S. Gatt and J. Schrevel Photosensitized inactivation of Plasmodium Falciparum and Babesia Divergens infected erythrocytes in whole blood by pheophorbide derivatives Vox Sanguinis (1997).
A. Dagan, S. Gatt, S. Cerbu-Karabat, J.C. Mazière, C. Mazière, R. Santus, E.L. Engelhardt, K.A. Yeh, C. C. Stobbe, M.C. Fenning and J. D. Chapman, Uptake by cells and photosensitizing effectiveness of novel pheophorbide derivatives in vitro Int. J. Cancer 63: 831–839 (1995).
D.K. Miller, E. Griffiths, J. Lenard and R.A. Firestone, Cell killing by lysosomotropic detergents, J. Cell Biol., 97: 1841 (1983).
P. Morlière, R. Santus, M. Bazin, E. Kohen, V. Carillet, F. Bon, J. Rainasse and L. Dubertret, Is Rhodamine 123 a photosensitizer ? Photochem. Photosssbiol., 52: 703 (1990).
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Gèze, M., Gaullier, JM., Bazin, M., Santus, R. (1996). Sub-Cellular Localization of Second Generation PDT Photosensitizers Studied by Microspectrofluorometry. In: Kohen, E., Hirschberg, J.G. (eds) Analytical Use of Fluorescent Probes in Oncology. NATO ASI Series, vol 286. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5845-3_11
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DOI: https://doi.org/10.1007/978-1-4615-5845-3_11
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