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Microcirculation and the Vascular Control of the Testis

  • Chapter
The Fate of the Male Germ Cell

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 424))

Summary

The arterial blood supply of the human testicular parenchyma within a testicular lobule demonstrates a segmental organization. In correlation to Leydig cells and seminiferous tubules within such a testicular tissue segment the capillary pathway was subdivided into three parts, namely arterial side inter-Leydig cell capillaries, intramural capillaries and venous side inter-Leydig cell capillaries. The organization, ultrastructure and functional aspects of the human testicular microvasculature were studied in detail. The casting preparation demonstrated a tightly organized vascular network. Computer aided 3-D reconstructions revealed that the capillary pathway in the human testis interconnects Leydig cells and seminiferous tubules. Electron microscopically the endothelial cells (EC) of arterial- and venous side inter-Leydig cell capillaries as well as of intertubular capillaries free of Leydig cells were of the continuous type without fenestrations. The intramural capillaries consisted of non-fenestrated and fenestrated sections. In all cases studied, the fenestrations faced the germinal epithelium. Transcytotic vesicles were numerous in the EC of inter-Leydig cell capillaries and in the non-fenestrated part of intramural capillaries. Leydig cells and Sertoli cells demonstrated immunostaining for vascular endothelial growth factor (VEGF) and its receptors flt-1 and KDR. In agreement with data obtained by RTPCR analyses, human testicular capillaries were negative for VEGF but positive for its receptors. Immunohistochemically Leydig cells and Sertoli cells were also positive for Big-Endothelin and Endothelin-1. Endothelin receptors ЕТ-А and ET-B were localized in Leydig cells while Sertoli cells showed only ЕТ-А immunostaining. Testicular blood vessels and peritubular cells were only positive for ET-B. Androgen receptors could be localized on the arterial side of the human testicular microvasculature and on intramural capillaries, whereas the intralobular veins were negative. In contrast, estrogen receptors were found in all parts of testicular vasculature. From these results we conclude: 1) In the human testis, capillaries interconnect Leydig cell clusters and seminiferous tubules in a serial manner. A part of the capillaries runs in between the layers of the lamina propria and therefore represents the capillarization of human seminiferous tubules. It was presumed that the multilayered lamina propria requires its own capillary supply which may allow a rapid exchange of hormones and other nutritious substances between the microvasculature and the germinal epithelium. 2) VEGF produced and released from Leydig and Sertoli cells could act as a paracrine factor on the testicular microvasculature which possesses VEGF receptors and could modulate the permeability of capillaries in the adult testis, e.g. by fenestration of intramural capillaries. 3) The presence of androgen receptors (AR) and estrogen receptors (ER) on the testicular vasculature indicate that the testicular microvasculature is involved in the local endocrine and paracrine regulation of spermatogenesis. Particularly the presence of AR on the arterial side and on intramural capillaries leads to the assumption that androgens could influence the blood supply to Leydig cells and seminiferous tubules. 4) Leydig cells and Sertoli cells could also influence the blood flow via the potent vasoconstrictor ET-1.

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References

  • Arai H, Hon i S, Aramori I, Ohkubo H & Nakanishi S 1990 Cloning and expression of a cDNA encoding an endothelin receptor. Nature 348: 730–732.

    Article  PubMed  CAS  Google Scholar 

  • Benahmed M 1996 Growth factors and cytokines in the testis. In: Male Infertility, pp 55–95. Ed Comhaire FH, Chapman & Hall, London.

    Google Scholar 

  • Bennet HS, Luft JH & Hampton JC 1959 Morphological classification of vertebrate blood capillaries. American Journal of Physiology 196: 381–390.

    Google Scholar 

  • Bergh A, Damber JE & Widmark A 1988 Hormonal control of testicular blood flow, microcirculation and vascular permeability. In: Molecular and Cellular Endocrinology of the Testis, pp 132–133. Eds Cooke BA & Sharpe RM, Raven Press, New York.

    Google Scholar 

  • Bergh A & Damber JE 1992 Immunohistochemical demonstrations of androgen receptors on testicular blood vessels. International Journal of Andrology 15: 425–434.

    Article  PubMed  CAS  Google Scholar 

  • Bergh A & Damber JE 1993 Vascular controls in testicular physiology. In: Molecular Biology of the Male Reproductive System, pp 439–468. Ed de Kretser D, Academic Press Inc.

    Google Scholar 

  • Boocock CA, Charnock-Jones DS, Sharkey AM, McLaren J, Barker PJ, Wrigth KA, Twentyman PR, & Smith SK 1995 Expression of vascular endothelial growth factor and its receptors fit and KDR in ovarian carcinoma. Journal of the National Cancer Institute 87: 506–16.

    Article  PubMed  CAS  Google Scholar 

  • Breier G, Albrecht U, Sterrer S & Rissau W 1992 Expression of vascular endothelial growth factor during embrionic angiogenesis and endothelial cell differentiation. Development 114: 521–532.

    PubMed  CAS  Google Scholar 

  • Brown LF, Yeo KT, Berse B, Yeo TK, Senger DR, Dvorak HF & Van De Water L 1992 Expression of vascular permeability factor (vascular endothelial growth factor) by epidermal keratinocytes during wound healing. Journal of Experimental Medicine 176: 1375–1379.

    Article  PubMed  CAS  Google Scholar 

  • Chang CS, Kokontis J & Liao ST 1988 Molecular cloning of human and rat complementary DNA encoding androgen receptors. Science 240: 324–326.

    Article  PubMed  CAS  Google Scholar 

  • Conn G, Soderman DD, Schaeffer MT, Wile M, Hatcher VB & Thomas KA 1990 Purification of a glycoprotein vascular endothelial cell mitogen from a rat gliona-derived cell line Proceedings of the National Academy of Sciences USA, 87: 1323–1327.

    CAS  Google Scholar 

  • Connolly DT, Olander JV, Heuvelnan D, Nelson R, Monsell R, Siegel N, Haymore BL, Leimgruber R, & Feder J 1989 Human permeability factor. Isolation from u937 cells. Journal of Biological Chemistry 264: 20017–20024.

    PubMed  CAS  Google Scholar 

  • Connolly DT 1991 Vascular permeability factor: A unique regulator of blood vessel function. Journal of Cellular Biochemistry 47: 219–223.

    Article  PubMed  CAS  Google Scholar 

  • Conte D, Questino P. Fillo N, Isidori A & Romanelli F 1993 Endothelin stimulates testosterone secretion by rat Leydig cells. Journal of Endocrinology 136: R1–R4.

    Article  PubMed  CAS  Google Scholar 

  • Cozza EN, Chiou S & Gomez-Sanchez CE 1992 Endothelin-1 potentiation of angiotensin II stimulation of aldosterone production. American Journal of Physiology 262: R85–R89.

    PubMed  CAS  Google Scholar 

  • Damber JE, Selstam G & Wang J 1981 Inhibitory effect on estradiol-1713 on human chorionic gonadotrophin-induced increment of testicular blood flow and plasma testosterone concentration in rats. Biology of Reproduction 25: 555–559.

    Article  PubMed  CAS  Google Scholar 

  • Damber JE, Bergh A & Widmark A 1987 Effect of an LHRH-agonist on testicular microcirculation in hypophysectomized rats. International Journal of Andrology 10: 785–791.

    Article  PubMed  CAS  Google Scholar 

  • Damber JE. Bergh A & Widmark A 1989 Effects of hormones on testicular microvasculature. In Perspectives in Andrology, vol 53: pp 97–109. Ed. M. Serio, Raven Press New, York.

    Google Scholar 

  • Damber JE & Bergh A 1992 Testicular microcirculation-a forgotten essential in andrology? International Journal of Andrology 15: 285–292.

    Article  PubMed  CAS  Google Scholar 

  • Damber JE, Madocks S, Widmark A & Bergh A 1992 Testicular blood flow and vasomotion can be maintained by testosterone in Leydig cell-depleted rats. International Journal of Andrology 15: 385–393.

    Article  PubMed  CAS  Google Scholar 

  • Davenport AP, Cameron 1T, Smith SK & Brown MJ 1991 Binding sites for iodinated endothelin-1, endothelin-2 and endothelin-3 demonstrated on human uterine glandular epithelial cells by quantitative high-resolution autoradiography. Journal of Endocrinology 129: 149–154.

    CAS  Google Scholar 

  • Davidoff MS, Breucker H, Holstein AF & Seidl K 1990 Cellular architecture of the lamina propria of human seminiferous tubules. Cell and Tissue Research 262: 253–261.

    Article  PubMed  CAS  Google Scholar 

  • Ergün S, Sting’ J & Holstein AF 1994a Segmental angioarchitecture of the testicular lobule in man. Andrologia 26: 143–150.

    Article  Google Scholar 

  • Ergün S, Stingl J & Holstein AF 1994b Microvasculature of the human testis in correlation to Leydig cells and seminiferous tubules. Andrologia 26: 235–262.

    Article  Google Scholar 

  • Ergün S & Ungefroren H 1995a Androgen and Estrogen receptors in the microvasculature of the human testis. Acta Anatomica 152: 278–279.

    Google Scholar 

  • Ergün S. Ungefroren H & Holstein AF 1995b Vasoaktive Substanzen im menschlichen Hoden-Hinweise auf para-und autokrine Regulation der testikulären Mikrozirkulation. Verhandlungen der Anatomischen Gesellschaft Vol 177 (Suppl): 14–15.

    Google Scholar 

  • Ergün S, Killic N, Fiedler W & Mukhopadhyay AK 1996? Vascular endothelial growth factor and its receptors in the human testicular tissue. Miniposter, 9th Eur. Workshop on Molecular and Cellular Endocrinology of the Testis, Geilo, Norway, c17.

    Google Scholar 

  • Ergün S, Davidoff M & Holstein AF 1996b Capillaries in the lamina propria of human seminiferous tubules are partly fenestrated. Cell and Tissue Research, 286: 93–102.

    Article  Google Scholar 

  • Ergün S, Davidoff M & Holstein AF 1996b Capillaries in the lamina propria of human seminiferous tubules are partly fenestrated. Cell and Tissue Research, 286: 93–102.

    Article  Google Scholar 

  • Fantoni G, Morris PL, Forti G, Vannelli GB, Orlando C, Barni T, Sestini R, Danza G & Maggi M 1993 En-dothelin-l: a new autocrine/paracrine factor in rat testis. American Journal of Physiology 265: E267–E274.

    PubMed  CAS  Google Scholar 

  • Fawcett DW, Heidger PJ & Leak LV 1969 The lymph-vascular system of the interstitial tissue of the testis as re-vealed by electron microscopy. Journal of Reproduction and Fertility 19: 109–119.

    Article  PubMed  CAS  Google Scholar 

  • Fawcett DW, Leak LV & Heidger PJ 1970 Electron microscopic observations on the structural components of the blood-testis barrier. Journal of Reproduction and Fertility, Suppl. 10: 105–119.

    CAS  Google Scholar 

  • Ferrara N & Henzel WJ 1989 Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. Biochemical and Biophysical Research Communications 161: 850–858.

    Article  Google Scholar 

  • Filippini A, Tripiciano A. Palombi F, Teti A, Paniccia R, Stefanini M & Ziparo E 1993 Rat testicular myoid cells respond to endothelin: characterization of binding and signal transduction pathway. Endocrinology 133: 1789–1796.

    Article  PubMed  CAS  Google Scholar 

  • Folkman J 1986 How is blood vessel growth regulated in normal and neoplasmatic tissue? G.H.A. Clowes memorial award lecture. Cancer Research 46: 467–473.

    PubMed  CAS  Google Scholar 

  • Folkman J & Shing Y 1992 Angiogenesis. Journal of Biological Chemistry 267: 10931–10934.

    CAS  Google Scholar 

  • Gospodarowicz D, Abraham JA & Schilling J 1989 Isolation and characterization of a vascular endothelial cell mitogen produced by pituitary derived folliculo stelate cells. Proceedings of the National Academy of Sciences USA 86: 7311–7315.

    Article  CAS  Google Scholar 

  • Hammami MM, Haq A & AlSedairy S 1994 The level of endothelin-like immunoreactivitiy in seminal fluid correlates positively with semen volume and negatively with plasma gonadotrophin levels. Clinical Endocrinology 40: 361–366.

    Article  PubMed  CAS  Google Scholar 

  • Hanahan D & Folkman J. 1996 Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell 86: 353–364.

    Article  PubMed  CAS  Google Scholar 

  • Holstein AF, Orlandini GE & МMöller R 1979 Distribution and fine structure of the lymphatic system in the human testis. Cell and Tissue Research 200: 15–27.

    Article  PubMed  CAS  Google Scholar 

  • Holstein AF 1988 In: Illustrated Pathology of Human Spermatogenesis, pp 76–116. Eds Holstein AF, Rosen-Runge EC, Schirren C, Grosse, Berlin.

    Google Scholar 

  • Hundeiker M & Keller L 1963 Die Gefäßarchitektur des menschlichen Hodens. Morphologisches Jahrbuch 105: 2073.

    Google Scholar 

  • Hundeiker М & Mullert LV 1966 Vermeidbare Risiken bei der Hodenbiopsie. Der Hautarzt 17: 546–547.

    CAS  Google Scholar 

  • Hundeiker M 1971 Die Kapillaren im Hodenparenchyn. Archive für klinische und experimentelle Dermatologie 239: 426–435.

    Google Scholar 

  • Jakeman LB, Winer.1, Bennet GL, Altar CA, & Ferrara N 1992. Binding sites for vascular endothelial growth fac-tor are localized on endothelial cells in adult rat tissues. Journal of Clinical Investigation 89: 244–253.

    Article  PubMed  CAS  Google Scholar 

  • Keck PJ, Hauser SD, Krivi G, Sanzo K, Warren T, Feder J & Connolly DT 1989 Vascular permeability factor, an endothelial cell mitogen related to PDGF. Science 246: 1309–1312.

    Article  PubMed  CAS  Google Scholar 

  • Jukov V. Pajusola K, Kaipainen A, et al. 1996 A novel vascular endothelial growth factor, VEGF-C is a ligand for the Flt-4 (VEGFR-3) and KDR (VEGFR-2) receptor tyrosine kinases. EMBO Journal 15: 290–298

    Google Scholar 

  • Klagsbrun M & D’Amore P1996 Vascular endothelial growth factor and its receptors. Cytokine and Growth Fac-tor Reviews 7(3): 259–270.

    Google Scholar 

  • Kormano M 1967a An angiographic study of the testicular vasculature in the postnatal rat. Zeitschrift für Anatomie und Entwicklungsgeschichte 126: 138–153.

    Article  CAS  Google Scholar 

  • Kormano M 1967b Dye permeability and alkaline phosphatase activity of testicular capillaries in the postnatal rat. Histochemie 9: 327–338.

    Article  CAS  Google Scholar 

  • Kormano M & Souranta H 1971a Microvascular organisation of the adult human testis. Anatomical Records 170:31–40.

    Article  CAS  Google Scholar 

  • Kormano M & Souranta H 1971b An angiographic study of the arterial pattern of the human testis. Anatomischer Anzeiger 128:69–76.

    CAS  Google Scholar 

  • Koseki C 1989 Autoradiographic distribution in rat tissues of binding sites for endothelin: a neuropeptide? American Journal of Physiology 256: R858–R866.

    PubMed  CAS  Google Scholar 

  • Lee J, Gray A, Yuan J, Louh SM, Avraham H & Wood W 1996 Vascular endothelial growth factor-related protein: A ligand and specific activator of the tyrosine kinase receptor Flt-4. Proceedings of the National Academy of Sciences USA 93: 1988–1992.

    Article  CAS  Google Scholar 

  • Leung DW, Cachianes G, Kuang WJ, Goeddel DV & Ferrara N 1989 Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246: 1306–1309.

    Article  PubMed  CAS  Google Scholar 

  • Maddocks S & Sharpe RM 1989 Dynamics of testosterone secretion by the rat testis: Implications for measurements of intratesticular levels of testosterone. Journal of Endocrinology 120: 323–329.

    Article  Google Scholar 

  • Maggi M, Bami T. Orlando C, Fantoni G, Finetti G, Vannelli GB, Mancina R, Gloria L, Bonaccorsi L, Yanagisawa M. & Forti G 1995 Endothelin-1 and its receptors in human testis. Journal of Andrology 16: 213–224.

    PubMed  CAS  Google Scholar 

  • Meyerhoffer A, Sinha Hikim AP, Bartke A & Russel LD 1989 Changes in the testicular microvasculature during photoperiod-related seasonal transition from reproductive quiescence to reproductive activity in the adult golden hamster. Anatomical Record 224: 495–507.

    Article  Google Scholar 

  • Meyerhoffer A & Bartke A 1990 Developing testicular microvasculature in the golden hamster. Mesocricetus aura-tus: a model for angiogenesis under physiological conditions. Acta Anatomica 139: 78–85.

    Google Scholar 

  • Miles AA & Miles EM 1952 Vascular reactions to histamine, histamin liberators, or leukotoxins in the skin of guinea pigs. Journal of Physiology 118: 228–257.

    PubMed  CAS  Google Scholar 

  • Miller I 1956 Kanälchen and Capillararchitektonik des Rattenhodens. Zeitschrift fir Zellforschung 45: 522–537.

    Google Scholar 

  • Olofsson B, Pajusola K, Kaipainen A, et al. 1996 Vascular endothelial growth factor B, a novel growth factor for endothelial cells. Proceedings of the National Academy of Sciences USA 93: 2576–2581.

    Article  CAS  Google Scholar 

  • Plate KH, Breier G, Weich H.A & Risau W 1992 Vascular endothelial growth factor is a Potential tumour angiogenesis factor in human gliomas in vivo. Nature 359: 845–848.

    Article  PubMed  CAS  Google Scholar 

  • Plate KH, Breier G, Millauer B, Ullrich A & Risau W 1993 Up-regulation of vascular endothelial growth factor nad its cognate receptors in a rat glioma model of tumour angiogenesis. Cancer Research 53: 5822–5827.

    PubMed  CAS  Google Scholar 

  • Qu-Hong, Nagy JA, Senger DR, Dvorak HF & Dvorak AM 1995 Ultrastructural localization of vascular permeability/vascular endothelial growth factor (VPF/VEGF) to the abl uminal plasma membrane and vesiculovacuolar organelles of tumor microvascular endothelium. Journal of Histochemistry and Cytochemistry 43: 381–389.

    Article  PubMed  CAS  Google Scholar 

  • Quinn TP, Peters KG, De VC, Ferrara N & Williams LT 1993 Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proceedings of the National Academy of Sciences USA 90: 7533–7537.

    Article  CAS  Google Scholar 

  • Roberts WG & Palade GE 1995 Increased microvascular permeability and endothelial fenestration induced by vascular endothelial growth factor. Journal of Cell Science 108: 2369–2379.

    PubMed  CAS  Google Scholar 

  • Sakaguchi H, Kozuka M, Hirose S. Ito Т & Hagiwara H 1992 Properties and localization of endothelin-l-specific receptors in rat testicles. American Journal of Physiology 263: R15–R18.

    PubMed  CAS  Google Scholar 

  • Schnittler HJ, Wilke A, Gress TH, Suttorp N & Drenckhahn D 1990 Role of actin and myosin in the control of paracellular permeability in pig, rat and human vascular endothelium. Journal of Physiology 431: 379–401.

    PubMed  CAS  Google Scholar 

  • Senger DR, Van De Water L. Brown L. Nagy J. Yeo KT, Yeo TK, Berse B, Jackman R, Dvorak A & Dvorak H 1993 Vascular permeability factor (VPF, VEGF) in tumor biology. Cancer Metastasis Reviews 12: 303–324.

    Article  PubMed  CAS  Google Scholar 

  • Setchell BP 1990 Local controls of testicular fluids. Reproduction, Fertility and Development 2: 291–309.

    Article  CAS  Google Scholar 

  • Setchell BP 1994 Anatomy, vasculature, innervation, and fluids of the male reproductive tract. In:) The Physiology of Reproduction, pp 1065–1175. Eds Knobil E, Neill J et al. Raven Press, New York

    Google Scholar 

  • Setchell BP & Sharpe RM 1981 The effect of human chorionic gonadotrophin on capillary permeability, extracel-lular fluid volume and flow of lymph and blood in the testis of rats. Journal of Endocrinology 91: 245–254.

    Article  PubMed  CAS  Google Scholar 

  • Setchell BP & Rommerts FFG 1986 The importance of Leydig cells in the vascular response to hCG in rats. Inter-national Journal of Andrology 8:436–440.

    Article  Google Scholar 

  • Sharma OP, Flores JA, Leong DA & Veldhuis JD 1994 Mechanisms by which endothelin-1 stimulates increased cytosolic free calcium ion concentrations in single rat Sertoli cells. Endocrinology 135: 127–134.

    Article  PubMed  CAS  Google Scholar 

  • Sharpe RM 1986 Paracrine control of the testis. Clinics in Endocrinology and Metabolism, 15: 185–207.

    Article  PubMed  CAS  Google Scholar 

  • Sharpe RM 1990 Intratesticular control of steroidogenesis. Clinical Endocrinology 33: 787–807.

    Article  PubMed  CAS  Google Scholar 

  • Shibuya M, Yamaguchi S, Yamane A, Ikeda T. Tojo A, Matsushime H & Sato M 1990 Nucleotide sequence and expression of a novel human receptor type tyrosin kinase gene (fit) closly related to the fms family. Onco-gene 5: 519–524.

    CAS  Google Scholar 

  • Shifren JL, Doldi N, Ferrara N, Mesiano S & Jaffe RB 1994 In the human fetus, vascular endothelial growth factor is expressed in epithelial cells and myocytes, but not vascular endothelium: implication for mode of action. Journal of Clinical Endocrinology and Metabolism 79: 316–22.

    Article  PubMed  CAS  Google Scholar 

  • Simonson MS & Dunn MJ 1990 Cellular signaling by peptides of the endothelia gene family. FASEB Journal 4: 2989–3000.

    PubMed  CAS  Google Scholar 

  • Simionescu N & Simionescu M 1987 Receptor-mediated transcytosis of albumin: Identification of albumin binding proteins in the plasma membrane of capillary endothelium. In: Proceedings of the IV World Congress on Microcirculation, Elsevier, Amsterdam and New York.

    Google Scholar 

  • Simionescu N & Simionescu M 1988 The cardiovascular system. In: Cell and Tissue Biology, pp. 355–398. Ed Leon Weiss, Urban and Schwarzenberg Baltimore-Munich.

    Google Scholar 

  • Suzuki F 1982 Microvasculature of the mouse testis and excurrent duct system. American Journal of Anatomy 163: 309–325.

    Article  PubMed  CAS  Google Scholar 

  • Suzuki F & Nagano Т 1986 Microvasculature of the human testis and excurrent duct system. Cell and Tissue Research 243: 79–89.

    Article  PubMed  CAS  Google Scholar 

  • Tan JA, Joseph DR, Quarmby VE, Lubahn DB, Sar M, French FS & Wilson EM 1988 The rat androgen receptor: primary structure, autoregulation of its mesenger ribonucleic acid and immunocytochemical localization of the receptor protein. Molecular Endocrinology 2: 1276–1285.

    Article  PubMed  CAS  Google Scholar 

  • Takagi H, King GL & Aiello LP 1996 Identification and characterization of vascular endothelial growth factor receptor (Fit) in bovine retinal pericytes. Diabetes 45: 1016–1023.

    Google Scholar 

  • Terman BI, Carrion ME, Kovacs E, Rasmusen BA, Eddy RL & Shows TB 1991 Identification of new endothelial cell growth factor receptor tyrosin kinase. Oncogene 6: 1677–1683.

    PubMed  CAS  Google Scholar 

  • Terman BI, Dougher Vermazen M, Carrion ME, Dimitrov D, Armellino DC, Gospodarowicz D & Bohlen P 1992 Identification of the KDR tyrosin kinase as a receptor for vascular endothelial cell growth factor. Biochemical and Biophysical Research Communications 187: 1579–1586.

    Article  PubMed  CAS  Google Scholar 

  • Tischer E, Mitchell R, Hartman T, Silva M, Gospodarowicz D, Fiddes JC & Abraham JA 1991 The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. Journal of Biological Chemistry 266: 11947–11954.

    PubMed  CAS  Google Scholar 

  • Viglietto G, Romano A, Maglione D, Rambaldi M, Paoletti I, Lago CT, Califano D, Monaco C, Mineo A, Santelli G, Manzo G, Botti G, Chiappetta G & Persico MG 1996 Neovascularization in human germ cell tumors correlates with a marked increase in the expression of the vascular endothelial growth factor but not the placenta-derived growth factor. Oncogene 13: 577–87.

    PubMed  CAS  Google Scholar 

  • Weerasooriya TR & Yamamoto T. 1985 Three-dimensional organisation of the vasculature of the rat spermatic cord and testis. Cell and Tissue Research 241: 317–323.

    Article  PubMed  CAS  Google Scholar 

  • Weissberg PL, Witchell C. Davenport AP, Hesketh TR & Metcalfe JC 1990 The endothelin peptide ET-1, ET-2, ET-3 and sarafotoxin 56b are co-mitogenic with platelet-derived growth factor for vascular smooth muscle cells. Arteriosclerosis 85: 257–262.

    Article  CAS  Google Scholar 

  • Widmark A. Damber JE & Bergh A. 1986 The relationship between human chorionic gonadotrophin-induced changes in testicular microcirculation and the formation of testicular interstitial fluid. Journal of Endocrinology 109: 419–425.

    Article  PubMed  CAS  Google Scholar 

  • Wolff J & Merker HJ 1966 Ultrastruktur und Bildung von Poren im Endothel von porösen und geschlossenen Kapillaren. Zeitschrift für Zellforschung 73: 174–191.

    Article  CAS  Google Scholar 

  • Xu D, Emoto N & Giaid A 1994 A membrane-bound metalloprotease that catalyzes the proteolytic activation of big endothelin-1. Cell 78: 473–485.

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi TP, Dumont DJ, Conlon RA, Breitman ML& Rossant J 1993 flk-1, an fit-related receptor tyrosine kinase is an early marker for endothelial cell precursors. Development 118: 489–498.

    Google Scholar 

  • Yanagisawa M, Kurihara H, Kimura S. Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K & Masaki T 1988 A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332: 411–415.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Anatomy, University of Hamburg (UKE), Martinistr. 52, 20246, Hamburg, Germany

    S. Ergün, N. Kilic, S. Harneit, H. Ungefroren, M. Davidoff & A. F. Holstein

  2. lnstitute for Hormone and Fertility Research, University of Hamburg, Grandweg 64, 22529, Hamburg, Germany

    H. J. Paust & A. Mukhopadhyay

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  1. S. Ergün
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  1. Institute for Hormone and Fertility Research, University of Hamburg, Hamburg, Germany

    Richard Ivell

  2. Anatomical Institute, University of Hamburg, Hamburg, Germany

    Adolf-Friedrich Holstein

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Ergün, S. et al. (1997). Microcirculation and the Vascular Control of the Testis. In: Ivell, R., Holstein, AF. (eds) The Fate of the Male Germ Cell. Advances in Experimental Medicine and Biology, vol 424. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5913-9_32

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