Abstract
Most protocols for gene therapy which correct metabolic defects involve the use of retrovirus vectors to introduce normal cellular genes into mutant somatic tissue (1–2). Although retroviruses have many advantages in gene therapy, such vectors containing endogenous promoters readily undergo deletion and rearrangement as well as recombination, which makes them unstable and potentially pathogenic (3–4). Human adenoviruses have been studied as possible helper independent gene transfer and expression vectors (5–8). The aprt gene is a good model system for studying the parameters of gene therapy and gene transfer. It is constitutively expressed in all cell types (9), its deficiency does not affect cell morphology or cell growth in culture; it is small (2.8 kb), and conversion from aprt− to aprt+ is easily selected. Many aprt− mutant cell lines are available (10) and can be used as target cells for in vitro studies.
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© 1991 Plenum Press, New York
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Wang, Q., Konan, V., Taylor, M.W. (1991). Expression of the APRT Gene in an Adenovirus Vector System as a Model for Studying Gene Therapy. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_14
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DOI: https://doi.org/10.1007/978-1-4615-7703-4_14
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