Methods for the Isolation and Determination of Glutamate, Glutamine, Aspartate, and γ-Aminobutyrate in Brain

Gaitonde, M. K.

doi:10.1007/978-1-4615-7751-5_13

M. K. Gaitonde³

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12 Citations

Abstract

With the advent of various types of chromatography, a wide choice of methods is now available for the resolution and isolation of amino acids in complex mixtures. For quantitative analysis, paper chromatography and ion-exchange chromatography are the methods of choice. One-dimensional rather than two-dimensional paper chromatography is preferred as a routine procedure, samples being pretreated with ion-exchange resins to obtain groups of amino acids. Reliable methods for the isolation of the amino acids in the brain after paper chromatography have been published (Gaitonde, 1961; Gaitonde et al., 1965; Robinson and Williams, 1965).

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References

Benson, J. V., Gordon, M. J., and Patterson, J. A. (1967) Anal. Biochem. 18, 228.
Article CAS Google Scholar
Berl, S., Lajtha, A., and Waelsch, H. (1961) J. Neurochem. 7, 186.
Article CAS Google Scholar
Bray, G. A. (1960) Anal. Biochem. 1, 279.
Article CAS Google Scholar
Dickinson, J. C., Rosenblum, H., and Hamilton, P. B. (1965) Pediatrics 36, 2.
PubMed CAS Google Scholar
Doležalova, V., and Brada, Z. (1964) Clin. Chim. Acta 10, 34.
Article PubMed Google Scholar
Evans, G. M., Thompson, J., and Gaitonde M. K. (1972) Unpublished work.
Google Scholar
Fischer, F. G., and Bohn, H. (1955) Z. Physiol. Chem. 302, 278.
Article CAS Google Scholar
Gaitonde, M. K. (1961) J. Neurochem. 8, 234.
Article PubMed CAS Google Scholar
Gaitonde, M. K., and Arnfred, T. (1971) J. Neurochem. 18, 1971.
Article PubMed CAS Google Scholar
Gaitonde, M. K., and Kelly, R. (1972) J. Neurochem. 19, 377.
Article PubMed CAS Google Scholar
Gaitonde, M. K., and Nixey, R. W. K. (1972) Anal. Biochem. 50, 416.
Article PubMed CAS Google Scholar
Gaitonde, M. K., Dahl, D. R., and Elliott, K. A. C. (1965) Biochem. J. 94, 345.
PubMed CAS Google Scholar
Hall, T. C., and Cocking, E. C. (1965) Biochem. J. 96, 626.
PubMed CAS Google Scholar
Hamilton, P. B. (1945) J. Biol. Chem. 158, 375.
CAS Google Scholar
Hamilton, P. B. (1958) Anal. Chem. 30, 914.
Article CAS Google Scholar
Hamilton, P. B. (1962) Ann. N.Y. Acad. Sci. 102(i), 55.
PubMed CAS Google Scholar
Hamilton, P. B. (1963) Anal. Chem. 35, 2055.
Article CAS Google Scholar
Hamilton, P. B., and Van Slyke, D. D. (1943) J. Biol. Chem. 150, 231.
CAS Google Scholar
Hirs, C. H. W., Moore, S., and Stein, W. H. (1954) J. Am. Chem. Soc. 76, 6063.
Article CAS Google Scholar
Lajtha, A., Furst, S., Gerstein, A., and Waelsch, H. (1957) J. Neurochem. 1, 289.
Article PubMed CAS Google Scholar
MacFadyen, D. A. (1950) J. Biol. Chem. 186, 1.
PubMed CAS Google Scholar
MacLennan, D. H., Beevers, H., and Harley, J. L. (1963) Biochem. J. 89, 316.
PubMed CAS Google Scholar
Matheson, A. T., Tigane, E., and Hanes, C. S. (1961) Can. J. Biochem Physiol. 39, 417.
Article PubMed CAS Google Scholar
Meyer, H. (1957) Biochem. J. 67, 333.
PubMed CAS Google Scholar
Minard, F. N., and Mushahwar, I. K. (1966a) Life Sci. 5, 1409.
Article PubMed CAS Google Scholar
Minard, F. N., and Mushahwar, I. K. (1966b) J. Neurochem. 13, 1.
Article PubMed CAS Google Scholar
Moore, S., and Stein, W. H. (1948) J. Biol Chem. 176, 367.
PubMed CAS Google Scholar
Moore, S., and Stein, W. H. (1954) J. Biol. Chem. 211, 907.
PubMed CAS Google Scholar
Moore, S., Spackman, D. H., and Stein, W. (1958) Anal. Chem. 30, 1185.
Article CAS Google Scholar
Oreskes, I., Cantor, F., and Kupfer, S. (1965) Anal. Chem. 37, 1720.
Article PubMed CAS Google Scholar
Perry, T. L., Stedman, D., and Hansen, S. (1968) J. Chromatog. 38, 460.
Article CAS Google Scholar
Porcellati, G., and Thompson, R. H. S. (1957) J. Neurochem. 1, 340.
Article PubMed CAS Google Scholar
Rajagopalan, T. G., Moore, S., and Stein, W. H. (1966) J. Biol. Chem. 241, 4940.
PubMed CAS Google Scholar
Roberts, E. (1962) In Amino Acid Pools (J. T. Holden, ed.), Elsevier, Amsterdam, p. 517.
Google Scholar
Roberts, E., and Frankel, S. (1950) J. Biol. Chem. 187, 55.
PubMed CAS Google Scholar
Robinson, N., and Williams, C. B. (1965) Clin. Chim. Acta 12, 311.
Article CAS Google Scholar
Rosen, H. (1957) Arch. Biochem. Biophys. 67, 10.
Article PubMed CAS Google Scholar
Saifer, A. (1971) Anal. Biochem. 40, 412.
Article PubMed CAS Google Scholar
Spackman, D. H., Stein, W. H., and Moore, S. (1958) Anal. Chem. 30, 1190.
Article CAS Google Scholar
Stein, W. H., and Moore, S. (1954) J. Biol. Chem. 211, 915.
PubMed CAS Google Scholar
Tallan, H. H., Moore, S., and Stein, W. H. (1956) J. Biol. Chem. 219, 257.
PubMed CAS Google Scholar
Tallan, H. H. (1962) In Amino Acid Pools (J. T. Holden, ed.), Elsevier, Amsterdam, p. 517.
Google Scholar
Tallan, H. H., Moore, S., and Stein, W. (1954) J. Biol. Chem. 211, 927.
PubMed CAS Google Scholar
Turner, J. C. (1968) Internat. J. Appl. Radiat. Isotopes 19, 557.
Article CAS Google Scholar
Vega, A., and Nunn, P. B. (1969) Anal. Biochem. 32, 446.
Article PubMed CAS Google Scholar
Yemm, E. W., and Cocking, E. C. (1955) Analyst (Lond.) 80, 209.
Article CAS Google Scholar
Yoshino, Y., and Elliott, K. A. C. (1970) Can. J. Biochem. 48, 228.
Article PubMed CAS Google Scholar

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Medical Research Council Neuropsychiatry Unit, Surrey, England, UK
M. K. Gaitonde

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M. K. Gaitonde
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New York State Research Institute for Neurochemistry and Drug Addiction, Ward’s Island, New York, New York, USA
Neville Marks
Department of Biochemistry, Institute of Psychiatry, University of London, London, Great Britain
Richard Rodnight

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Gaitonde, M.K. (1974). Methods for the Isolation and Determination of Glutamate, Glutamine, Aspartate, and γ-Aminobutyrate in Brain. In: Marks, N., Rodnight, R. (eds) Research Methods in Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7751-5_13

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DOI: https://doi.org/10.1007/978-1-4615-7751-5_13
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