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Immobilization of Microbial Cells using Gelatin and Glutaraldehyde

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Enzyme Engineering

Abstract

Immobilized enzymes and cells (1,2) should be of considerable economical and practical importance. Therefore, since 1976 we have been working on techniques for immobilization of microbial whole cells (3,4). Entrapment in gelatin and crosslinking with glutaraldehyde has been employed successfully for the immobilization of Echerichia coli D816 cells for penicillin acylase activity and Streptomyces roseofulus Kc 13–575 cells for glucose isomerase.

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References

  1. YUAN, Z., LIU, S. & YUAN, J., “Immobilized Enzymes and AffinityChromatography”, Science Press, Beijing, China (1975).

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  2. MOSBACH, K. Meth. Enzymol. 44: (1976).

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  3. Prog. Biochem. Biophys. 2: 65 (1980).

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  4. WANG, Q., YE, X., ZHAO, F., LIU, G., LIU, G., OU, G., SHEN, C. & XU, J. Acta Biochimica Biophysica Sinica 12: 305 (1980).

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  5. JI, X., LI, H., ZENG, Y., BAI, P., GU, G., WANG, L. & XU, S.Progress Biochem. Biophys. j in press.

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Author information

Authors and Affiliations

  1. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai, China

    Q. Wang, X. Ji & Z. Yuan

Authors
  1. Q. Wang
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  2. X. Ji
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  3. Z. Yuan
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Editor information

Editors and Affiliations

  1. Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company, Osaka, Japan

    Ichiro Chibata

  2. Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Kyoto, Japan

    Saburo Fukui

  3. Department of Pharmacology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Lemuel B. Wingard Jr.

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© 1982 Plenum Press, New York

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Wang, Q., Ji, X., Yuan, Z. (1982). Immobilization of Microbial Cells using Gelatin and Glutaraldehyde. In: Chibata, I., Fukui, S., Wingard, L.B. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9290-7_44

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  • DOI: https://doi.org/10.1007/978-1-4615-9290-7_44

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9292-1

  • Online ISBN: 978-1-4615-9290-7

  • eBook Packages: Springer Book Archive

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