Skip to main content

Biochemical Characterization of a Clonal Line of Neuroblastoma

  • Chapter
Tissue Culture of the Nervous System

Part of the book series: Current Topics in Neurobiology ((CTNB))

Abstract

Study of the molecular mechanism of differentiation has been hampered by, among other difficulties, the complexity of the systems investigated, where several changes occur at the same time and overlap each other. The possibility of selecting a homogeneous cellular population in which only a limited step of cellular maturation occurs may provide a useful system for studying cell differentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Amano, T., Richelson, E., and Nirenberg, M. 1972. Neurotransmitter synthesis by neuroblastoma clones. Proc. Natl. Acad. Sci. 69:258–263.

    Article  PubMed  CAS  Google Scholar 

  • Augusti-Tocco, G. 1971. In F. A. Morris, P. G. Nelson, and F. H. Ruddle (eds.). Contributions of clonal systems to neurobiology; Neurosci. Res. Bull., Vol. 11.

    Google Scholar 

  • Augusti-Tocco, G., and Sato, G. H. 1969. Establishment of functional clonal lines of neurons from mouse neuroblastoma. Proc. Natl. Acad. Sci. 64:311–315.

    Article  PubMed  CAS  Google Scholar 

  • Augusti-Tocco, G., Sato, G. H., Claude, P., and Potter, D. D. 1970. Clonal cell lines of neurons, pp. 109–120. In H. A. Padykula (ed.) Control Mechanisms in the Expression of Cellular Phenotypes. Academic Press, New York.

    Google Scholar 

  • Augusti-Tocco, G., Casola, L., and Grasso, A. 1973. Neuroblastoma cells and 14-3-2, a brain specific protein. Submitted for publication.

    Google Scholar 

  • Bondy, S. C. 1972. Axonal migration of various ribonucleic acid species along the optic tract of the chick. J. Neurochem. 19:1769–1776.

    Article  PubMed  CAS  Google Scholar 

  • Brown, J. C. 1972. Surface glycoprotein characteristic of the differentiated state of neuroblastoma C-1300 cells. Exptl. Cell Res. 69:440–442.

    Article  Google Scholar 

  • Buck, C. A., Glick, M. C., and Warren, L. 1970. A comparative study of glycoproteins from the surface of control and Rous sarcoma virus transformed hamster cells. Biochemistry 9:4567–4576.

    Article  PubMed  CAS  Google Scholar 

  • Casola, L., Davis, G. A., and Davis, R. E. 1969. Evidence for RNA transport in rat optic nerve. J. Neurochem. 16:1037–1041.

    Article  PubMed  CAS  Google Scholar 

  • Colby, C., and Edlin, G. 1970. Nucleotide pool levels in growing, inhibited, and transformed chick fibroblast cells. Biochemistry 9:917–920.

    Article  PubMed  CAS  Google Scholar 

  • Darnell, J. E., Wall, R., and Tushinski, R. J. 1971. An adenylic acid-rich sequence in messenger RNA of Hela cells and its possible relationship to reiterated sites in DNA. Proc. Natl. Acad. Sci. 68:1321–1325.

    Article  PubMed  CAS  Google Scholar 

  • DeKaban, A. S., Patton, V. M., and Cain, D. F. 1971. Structural and biochemical maturation of the cerebral pallium in rabbit fetuses: morphogenesis and lipids. J. Neurochem. 18:2451–2459.

    Article  PubMed  CAS  Google Scholar 

  • Dravid, A. R., Pete N., and Mandel, P. 1971. An enzyme system in rat brain nuclei incorporating AMP into polyadenylate. J. Neurochem. 18:299–300.

    Article  PubMed  CAS  Google Scholar 

  • Edmonds, M., Vaughan, M. H., and Nakazato, H. 1971. Poly-adenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA in Hela cells: possible evidence for a precursor relationship. Proc. Natl. Acad. Sci. 68:1336–1340.

    Article  PubMed  CAS  Google Scholar 

  • Furshpan, E. J., and Potter, D. D. 1968. Low-resistance junctions between cells in embryos and tissue culture, pp. 95–127. In A. A. Moscona, and A. Monroy, (eds.). Current Topics of Developmental Biology, Vol. 3. Academic Press, New York.

    Google Scholar 

  • Giles, K. W., and Myers, A. 1965. An improved diphenylamine method for the estimation of DNA. Nature 206:93.

    Article  CAS  Google Scholar 

  • Goldschneider, I., and Moscona, A. A. 1972. Tissue-specific cell-surface antigens in embryonic cells. J. Cell Biol. 53:435–449.

    Article  PubMed  CAS  Google Scholar 

  • Ham, R. G. 1963. An improved nutrient solution for diploid Chinese hamsters and human cell lines. Exptl. Cell Res. 29:515–526.

    Article  PubMed  CAS  Google Scholar 

  • Jacob, M. 1971. RNA metabolism and differentiation of the central nervous system. In Abstracts 7th FEBS Meeting, Varna, p. 70.

    Google Scholar 

  • Lee, S. Y., Mendecki, J., and Brawerman, G. 1971. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Prod. Natl. Acad. Sci. 68:1331–1335.

    Article  CAS  Google Scholar 

  • Lowry, O. H., Rosebrough, N. F., Farr, A. L., and Randall, R. G. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    PubMed  CAS  Google Scholar 

  • Lumsden, C. E. 1968. Nervous tissue in culture, pp. 67–140. In G. H. Bourne (ed.) The Structure and Function of Nervous Tissue. Academic Press, New York.

    Google Scholar 

  • Mejbaum, W. 1939. Ãœber die Bestimmung kleiner Pentosemengen, insbesondere in Derivaten der Adenylse. Hoppe-Seylers Z. Physiol. Chem. 258:117–120.

    Article  CAS  Google Scholar 

  • Nelson, P., Ruffner, W., and Nirenberg, M. 1969. Neuronal tumor cells with excitable membranes grown in vitro. Proc. Natl. Acad. Sci. 64:1004–1010.

    Article  PubMed  CAS  Google Scholar 

  • Olmsted, J. B., Carlson, K., Klebe, R., Ruddle, F., and Rosenbaum, J. 1970. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. 65:129–136.

    Article  PubMed  CAS  Google Scholar 

  • Penman, S. 1966. RNA metabolism in the Hela cell nucleus. J. Molec. Biol. 17:117–130.

    Article  PubMed  CAS  Google Scholar 

  • Scherrer, K., and Darnell, J. E. 1962. Sedimentation characteristics of rapidly labeled RNA from Hela cells. Biochem. Biophys. Res. Commun. 7:486–490.

    Article  PubMed  CAS  Google Scholar 

  • Schubert, D., and Jacob, F. 1970. 5-Bromodeoxyuridine-induced differentiation of a neuroblastoma. Proc. Natl. Acad. Sci. 67:247–254.

    Article  PubMed  CAS  Google Scholar 

  • Schubert, D., Humphreys, S., Baroni, C., and Cohn, M. 1969. In vitro differentiation of a mouse neuroblastoma. Proc. Natl. Acad. Sci. 64:316–323.

    Article  PubMed  CAS  Google Scholar 

  • Schubert, D., Humphreys, S., de Vitry, F., and Jacob, F. 1971. Induced differentiation of a neuroblastoma. Develop. Biol. 25:514–546.

    Article  PubMed  CAS  Google Scholar 

  • Sidman, R. L. 1972. Cell interactions in developing mammalian central nervous system, pp. 1–13. In L. G. Silvestri (ed.). Cell Interactions. North-Holland, Amsterdam and London.

    Google Scholar 

  • Siman-Tov, R. and Sachs, L. 1972. Enzyme regulation in neuroblastoma cells. Europ. J. Biochem. 30:123–129.

    Article  PubMed  CAS  Google Scholar 

  • Warren, L., Critchley, D., and Macpherson, I. 1972. Surface glycoproteins and glycolipids of chicken embryo cell transformed by a temperature-sensitive mutant of Rous sarcoma virus. Nature 235:275–278.

    Article  PubMed  CAS  Google Scholar 

  • Weber, K., and Osborn, M. 1969. The reliability of molecular weight determinations by dodecylsulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244:4406–4412.

    PubMed  CAS  Google Scholar 

  • Weber, M. J. 1972. Ribosomal RNA turnover in contact inhibited cells. Nature 235:58–61.

    Article  CAS  Google Scholar 

  • Weinberg, R. A., Loening, U., Williems, M., and Penman, S. 1967. Acrylamide gel electrophoresis of Hela cell nucleolar RNA. Proc. Natl. Acad. Sci. 58:1088–1095.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1973 Plenum Press, New York

About this chapter

Cite this chapter

Augusti-Tocco, G., Parisi, E., Zucco, F., Casola, L., Romano, M. (1973). Biochemical Characterization of a Clonal Line of Neuroblastoma. In: Sato, G. (eds) Tissue Culture of the Nervous System. Current Topics in Neurobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2904-6_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-2904-6_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-2906-0

  • Online ISBN: 978-1-4684-2904-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics