Summary
Two new bifunctional reagents suited for the step-wise cross-linking of cysteine and lysine residues in proteins are described. Application to lactate dehydrogenase yields a cross-link between cysteine-165 and lysine-179, which suggests an alternative mechanism by which the “essential” cysteine reacts. for the mapping of the environment of a known and well defined amino acid the use of semireversible bifunctional reagents is suggested.
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© 1977 Plenum Press, New York
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Trommer, W.E., Friebel, K., Kiltz, HH., Kolkenbrock, HJ. (1977). Synthesis and Application of New Bifunctional Reagents. In: Friedman, M. (eds) Protein Crosslinking. Advances in Experimental Medicine and Biology, vol 86A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3282-4_10
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DOI: https://doi.org/10.1007/978-1-4684-3282-4_10
Publisher Name: Springer, Boston, MA
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