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Long Term Perifusion of Rabbit Ovarian Follicles

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Ovarian Follicular and Corpus Luteum Function

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 112))

Abstract

Preovulatory follicles were dissected from ovaries of estrous rabbits. Four follicles were placed in each chamber of a perifusion system. Medium 199, containing 10% rabbit serum and antibiotics, eluted through the chambers at a rate of 1 ml/10 min. Every 6 h for 36 h, 1 µg of LH and 50 ng of FSH were injected into each chamber. P4, T and E2 were measured in the medium. P4 exhibited a peak after each of the injections at 6, 12, 18, 24 and 30 h. E2 and T gave major peaks after the injection at 0 time, with minor peaks after the injection at 6 h; thereafter T and E2 secretion gradually decreased to levels below those of baseline.

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Author information

Authors and Affiliations

  1. Departments of Animal Science and Physiology, Animal Genetics Laboratory, University of Illinois, Urbana, IL, USA

    C. D’Amato, J. Bahr, B. Stockert, F. Calvo & D. Kesler

Authors
  1. C. D’Amato
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  2. J. Bahr
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  3. B. Stockert
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  4. F. Calvo
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  5. D. Kesler
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Editor information

Editors and Affiliations

  1. Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland, USA

    Cornelia P. Channing

  2. Department of Biochemistry, University of Miami School of Medicine, Miami, Florida, USA

    John M. Marsh

  3. Center for Population Research, National Institute of Child Health and Human Development, Bethesda, Maryland, USA

    William A. Sadler

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© 1979 Plenum Press, New York

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D’Amato, C., Bahr, J., Stockert, B., Calvo, F., Kesler, D. (1979). Long Term Perifusion of Rabbit Ovarian Follicles. In: Channing, C.P., Marsh, J.M., Sadler, W.A. (eds) Ovarian Follicular and Corpus Luteum Function. Advances in Experimental Medicine and Biology, vol 112. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3474-3_28

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  • DOI: https://doi.org/10.1007/978-1-4684-3474-3_28

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3476-7

  • Online ISBN: 978-1-4684-3474-3

  • eBook Packages: Springer Book Archive

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