Abstract
A multidisciplinary program to elucidate the health and ecological effects of advanced fossil fuels use is currently underway at the Oak Ridge National Laboratory. The development of short-term bioassays applicable to the characterization of complex mixtures is an important aspect of the program. Studies reported here have been designed to identify constituents responsible for the mutagenicity of energy related materials. Observations made in the course of these studies may prove useful for designing biotesting methods suitable for the quantitative routine application to complex mixtures.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bock FG, Swain AP, Stedman RL: Carcinogenesis assay of subfraction of cigarette smoke condensate prepared by solvent-solvent separation of the neutral fraction. J Natl Cancer Inst 49:477–483, 1972
Bock FG, Swain AP, Stedman RL: Composition studies on tobacco. XLIV. Tumorpromoting activity of subfractions of the weak acid fraction of cigarette smoke condensate. J Natl Cancer Inst 47:429–436, 1971
Bock FG, Swain AP, Stedman RL: Bioassay of major fractions of cigarette smoke condensate by an accelerated technic. Cancer Res 29:584–587, 1969
Chamberlain WJ, Stedman RL: Fractionation of tabacco smoke condensate for chemical composition studies. In: The Chemistry of Tobacco and Tobacco Smoke (Schmeltz I, ed.). New York, Plenum Press, 1972, pp 99–105
Dong MW, Locke DC, Hoffmann D: Characterization of aza-arenes in basic organic portion of suspended particulate matter. Environ Sci and Tech 11(6):612–618, 1977
Epler JL, Clark BR, Ho C-h, Guerin MR, Rao TK: Short-term bioassay of complex organic mixtures: Part II, mutagenicity testing. Proc of Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures (these proceedings)
Gallegos EJ: Terpane-sterane release from kerogen by pyrolysis gas chromatography-mass spectrometry. Anal Chem 47(9):1524–1528, 1975
Goeckner NA: Western Illinois University, Macombe, Illinois, Oak Ridge National Laboratory Faculty Participant, 1977–1978, private communication
Griest WH, Guerin MR: Influence of tobacco type on smoke composition. In: Recent Advances in Tobacco Science, Vol 3. Tobacco Smoke: Its Formation and Composition. Proc 31st Tobacco Chemists’ Research Conference, 1977, pp 121–144
Guerin MR, Epler JL, Griest WH, Clark BR, Rao TK: Polycyclic aromatic hydrocarbons from fossil fuel conversion processes. Proc Second International Symposium on Polynuclear Aromatic Hydrocarbons (in press)
Guerin MR, Maddox WL, Stockely JR: Tobacco smoke inhalation exposure: concepts and devices. Proc Tobacco Smoke Inhalation Workshop on Experimental Methods in Smoking and Health Research. DHEW Pub No (NIH) 75–906:31–44, 1976
Guerin MR, Stokely JR: Proband-machine animal interactions in inhalation bioassays. Proc Tobacco Smoke Inhalation Workshop on Bioassay Models and Inhalation Toxicology. DHEW Pub (in press)
Jones AR, Guerin MR, Clark BR: Preparative-scale liquid chromatographic fractionation of crude oils derived from coal and shale. Anal Chem 49:1766–1771, 1977
Kier LD, Yamasaki E, Ames BN: Detection of mutagenic activity in cigarette smoke condensates. Proc Nat Acad Sci USA, Vol 71(10):4159–4163, 1974
McGill HC: The human model. Proc Tobacco Smoke Inhalation Workshop on Bioassay Models and Inhalation Toxicology, DHEW Pub (in press)
Mizusaki S, Okamoto H, Akiyama A, Fukuhara Y: Relation between chemical constituents of tobacco and mutagenic activity of cigarette smoke condensate. Mutat Res 48(3/4):319–325, 1977
Mizusaki S, Takashima T, Tomaru K: Factors affecting mutagenic activity of cigarette smoke condensate in Salmonella typhimurim TA1538. Mutat Res 48(l):29–36, 1977
Reist PC: Particle size and its role in physical and chemical interactions of tobacco smoke aerosol. Proc Tobacco Smoke Inhalation Workshop on Bioassay Models and Inhalation Toxicology. DHEW Pub (in press)
Rubin IB, Guerin MR: Chemical evaluation of the beeswax pellet implantation bioassay model for studies of environmental carcinogens. J Natl Cancer Inst 58(3): 641–644, 1976
Rubin IB, Guerin MR, Hardigree AA, Epier JL: Fractionation of synthetic crude oils from coal for biological testing. Envir Res 12:358–365, 1976
Schiller JE: Nitrogen compounds in coal derived liquids. Anal Chem 49(1): 2292–2294, 1977
Shultz JL, White CM, Schweighardt FK, Sharkey AG: Characterization of the heterocyclic compounds in coal liquefaction products. Part I: nitrogen compounds. Pittsburgh Energy Research Center Report PERC/RI-77/7, 1977
Snook ME, Arrendale RF, Higman HC, Chortyk 0T: Isolation of indoles and carbazoles from cigarette smoke condensate. Anal Chem 50(1):88–90, 1978
Swain AP, Bock FG, Cooper JE, Chamberlain WJ, Strange ED, Lakritz L, Stedman RL, Schmeltz I, Russell RB: Further fractionations of cigarette smoke condensate for bioassays. Weak acid and neutral subfractions and combinations of active fractions. Beitr Tabakforsch 7:1–7, 1973
Swain AP, Cooper JE, Stedman RL: Large scale fractionation of cigarette smoke condensate for chemical and biologic investigations. Cancer Res 29:579–583, 1969
Swain AP, Cooper JE, Stedman RL, Bock FG: Composition studies on tobacco XL. Large scale fractionation of the neutrals of cigarette smoke condensate using adsorption chromatography and solvent partitioning. Beitr Tabakforsch 5:109–114, 1969
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1978 Springer Science+Business Media New York
About this chapter
Cite this chapter
Guerin, M.R., Clark, B.R., Ho, Ch., Epler, J.L., Rao, T.K. (1978). Short-Term Bioassay of Complex Organic Mixtures: Part I, Chemistry. In: Waters, M.D., Nesnow, S., Huisingh, J.L., Sandhu, S.S., Claxton, L. (eds) Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures. Environmental Science Research, vol 15. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3611-2_10
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3611-2_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3613-6
Online ISBN: 978-1-4684-3611-2
eBook Packages: Springer Book Archive