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Monoclonal Antibodies to Rat Plasma Kininogen

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Kinins IV

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 198A))

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Summary

Spleen cells from Balb/c mice immunized with purified rat plasma kininogen were fused to P-3 mouse myeloma cells. Positive clones were identified by enzyme linked immunosorbent assay (ELISA), cloned successively two times with limiting dilution and expanded as ascites tumors. Five hybridomas were developed that produced monoclonal antibodies against plasma kininogen. Two of the secreted antibodies were of the IgG1(k) isotype and the remaining three were of the IgG1(λ), IgG2A(k) and IgM(k) isotypes respectively. The specificity of the monoclonal antibodies was confirmed by the immunoprecipitation of kininogen with the antibodies coupled to Sepharose-4B followed by SDS-polyacrylamide gel electrophoresis. These monoclonal antibodies recognize at least two distinct epitopes on rat plasma kininogen.

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References

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Authors and Affiliations

  1. Department of Biochemical Pharmacology, State University of New York at Buffalo, Buffalo, New York, 14260, USA

    Gurrinder S. Bedi & Nathan Back

Authors
  1. Gurrinder S. Bedi
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  2. Nathan Back
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Editor information

Editors and Affiliations

  1. Medical College of Georgia, Augusta, Georgia, USA

    Lowell M. Greenbaum Ph.D (President, IKC) (President, IKC)

  2. Medical University of South Carolina, Charleston, South Carolina, USA

    Harry S. Margolius Ph.D, M.D. (Vice President, IKC) (Vice President, IKC)

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© 1986 Plenum Press, New York

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Bedi, G.S., Back, N. (1986). Monoclonal Antibodies to Rat Plasma Kininogen. In: Greenbaum, L.M., Margolius, H.S. (eds) Kinins IV. Advances in Experimental Medicine and Biology, vol 198A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5143-6_18

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  • DOI: https://doi.org/10.1007/978-1-4684-5143-6_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5145-0

  • Online ISBN: 978-1-4684-5143-6

  • eBook Packages: Springer Book Archive

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