Some Properties of Bovine High Molecular Weight Kininogen

Abstract

The presence of two kininogens has been shown in human, dog, guinea pig, rabbit and rat plasma by Jacobsen (1–3), in horse plasma by Henriques et al.(4), and in bovine plasma by Yano et al. (5). Pierce and Webster (6) isolated two low molecular weight (LMW) human kininogens with the same molecular weight of about 50,000 but with their kinin sequences carboxyl-terminal in one case and internal in the other. After that, two kininogens of different molecular weight were isolated from human plasma by Jacobsen and Kritz (7). LMW bovine kininogen (kininogen-II) has been highly purified by the groups of Habermann (8) and of Suzuki (9), and it was found by us that the kinin segment, which was thought to lie in the central part of LMW kininogen molecule, was released by trypsin, snake venom kininogenase or hog pancreatic kallikrein but not by glass-or casein-activated bovine plasma kallikrein. High molecular weight (HMW) kininogen (kininogen-I), a sensitive substrate of bovine plasma kallikrein, was isolated from bovine plasma by Yano et al.(10), and it was found that there is a methionyl-lysyl-bradykinin sequence located both at the carboxyl terminus and inside of the polypeptide chain of bovine HMW kininogen (11).