Abstract
Observations of decreased spermatozoa motility after freezing have motivated investigations attempting to identify ultrastructural cell damage from this procedure. As a result, lesions to the plasma membrane, acrosome and certain flagellar elements have been documented.1,7,10,12 However, these studies have been performed on spermatozoa frozen in cryoprotector media. Therefore, their findings are the result of a dual process: Cryo-aggression and cryo-protection. A better understanding of these two processes as well as the effect of the cryoprotector medium alone without freezing is needed and may lead to improved efficacy of semen cryopreservation. Of the studies that have approached the problem in this way,8,9 none have quantified their results.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Behrman, S.J. Freeze preservation of human spermatozoa in liquid nitrogen vapor. Schweiz, Z. Gyn äk. Geburt sh; 2:307–313, 1971.
Bostrom, K. and Rubin, S.O. Destruction of spermatozoa in freezethawing of human semen. Scand. J. Urol. Nephrol., 7:120–122, 1973.
David, G., Bisson, J.P., Czyglik, F., Jouannet P. and Gernigon, C. Que doit comporter 1’examen du sperme ? Comment en interpreter les résultats ? Feuillets de Biologie, XV:76, 1974.
David, G. and Czyglik, F. Tolerance à la congelation du sperme humain en fonction de la qualité initiale du sperme. J. Gyn. Obst. Biol. Repr., 6:601–610, 1977.
Drevius, L.O. and Eriksson, H. Osmotic swelling of mammalian spermatozoa. Experimental Cell Research, 42:136–156, 1966.
Fawcett, D.W. The structure of the mammalian spermatozoon. Int. Rev. Ctyol., 7, 1958.
Friberg, J. and Nilsson, O. Motility and morphology of human sperms after freezing in liquid nitrogen. In: “Current Problems in Fertility”, Ingelman, Sundberg and Lunell, eds. Plenum Press, New-York-London, 1971.
Jones, R.C. Fertility and infertility in mammals in relation to sperm structure. In: The Biology of male gamete. J.G. Duckett and P.A. Racey, eds. Academic Press, London, pp. 343–365, 1975.
Matheson, G.W., Carlborg, L. and Gemzell, C. Frozen human semen for artificial insemination. Am. J. Obst. Gynec., 104:495–501, 1969.
Pedersen, H. and Lebech, P.E. Ultrastructural changes in the human spermatozoon after freezing for artificial insemination. Fertil. and Steril., 22:2, 1971.
Pedersen, H. The human spermatozoon. Dan. Med. Bull., .21 suppl. 1:3–36, 1974.
Schill, W.B. and Wolff, H. Ultrastructure of human sperm acrosome and determination of acrosin activity under conditions of semen preservation. Int. J. Fertil., 19:217–223, 1974.
Woolley, D.M. and Richardson, D.W. Ultrastructural injury to human spermatozoa after freezing and thawing. J. Repr od. Fert., 52:389–394, 1978.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 Plenum Press, New York
About this chapter
Cite this chapter
Escalier, D., Bisson, JP. (1980). Quantitative Ultrastructural Modifications in Human Spermatozoa after Freezing. In: David, G., Price, W.S. (eds) Human Artificial Insemination and Semen Preservation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8824-1_17
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8824-1_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8826-5
Online ISBN: 978-1-4684-8824-1
eBook Packages: Springer Book Archive