Abstract
Terminal deoxynucleotidyl transferase, TdT, is an unusual DNA polymerase that does not require a template and catalyzes the random addition of deoxynucleotidyl units to the 3’-OH ends of single DNA chains and of oligodeoxynucleotides. The enzyme was first identified as a distinct entity by Krakow et al. (1962), but it was purified from calf thymus and extensively characterized in the laboratory of F.J. Bollum (Yoneda and Bollum, 1965; Kato et al., 1967; Chang and Bollum, 1971a). For several years TdT was considered almost exclusively as a convenient tool for synthesizing DNA-like molecules (Bollum, 1966; Ratliff et al., 1967; Chang and Bollum, 1971b) and also we made large use of it in our Euratom program of preparing tailored polydeoxynucleotide substrates for the enzymes of DNA repair (Campagnari et al., 1973).
This publication is, in part, contribution n. 1786 of the Programme Biology, Radiation Protection and Medical Research, Directorate General XII of the Commission of the European Communities.
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References
Bekkering-Kuylaars, S. A. M., and Campagnari F., 1972, Purification of a DNA polymerase from calf Thymus nuclei, Biochem, Biophys, Acta, 272: 526.
Bollum, F. J., 1966, Biosynthetic polydeoxynucleotides, in; “Procedures in Nucleic Acid Research,” vol. 1, p. 577, G. L. Cantoni and D.R. Davies, eds., Harper and Row, New York.
Bollum, F. J., 1974, Terminal deoxynucleotidyl transferase, in:”Enzymes,” vol. 10, p. 145, P.D. Boyer, ed., Academic Press, New York.
Bollum, F. J., 1978, Terminal deoxynucleotidyl transferase; Biological Studies, in: “Advances in Enzymology,” vol. 47, p.347, A. Meister, ed., John Wiley and Son, New York.
Bollum, F. J., and Brown, M., 1979, A high molecular weight form of terminal deoxynucleotidyl transferase. Nature, 278; 191.
Boyum, A., 1968, Isolation of mononuclear cells and granulocytes from human blood, Scand. J. Lab. Inv., 21 (Suppl. 97); 77.
Campagnari, F., Clerici, L., and Bertazzoni, U., 1973, Studies in mammalian genetical biochemistry. Annual Rep. 1972 Joint Research Center, Ispra, EUR 5060; 403.
Chang, L. M. S. and Bollum, F. J., 1971, Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase, J. Biol. Chem., 246; 909.
Chang, L. M. S. and Bollum F. J., 1971, Enzymatic synthesis of oligodeoxynucleotides, Biochemistry, 10; 536.
Coleman, M. S., Hutton, J. J., De Simone, P., and Bollum, F. J., 1974, Terminal deoxyribonucleotidyl transferase in human leukemia, Proc. Natl. Acad. Sci. 71: 4404.
Coleman, M. S., Greenwood, M. F., Hutton, J. J., Bollum, F. J., 1976, Lampkin, B., and Holland, P., 1976, Serial observations on terminal deoxynucleotidyl transferase activity and lymphoblasts surface markers in acute lymphoblastic leukemia. Cancer Res., 36: 120.
Coleman, M. S., 1977, Terminal deoxynucleotidyl transferase: characterization of extraction and assay conditions from human and calf tissue. Arch. Biochem. Biophys., 182; 525.
Coleman, M. S., 1977, A critical comparison of commonly used procedures for the assay of terminal deoxynucleotidyl transferase in crude tissue extracts. Nucleic Acids Res., 4: 4305.
Deibel, M. R. Jr., and Coleman, M.S., 1979, Purification of a high molecular weight human terminal deoxynucleotidyl transferase, J. Biol. Chem., 254: 8634.
Donlon, J. A., Jaffe, E. S., and Braylan, R. C., 1977, Terminal deoxynucleotidyl transferase activity in malignant lymphomas. New Engl. J. Med., 297: 461.
Goldschneider, J., Gregoire, K. E., Barton, R. W., and Bollum, F. J., 1977, Demonstration of terminal deoxynucleotidyl transferase in thymocytes by immunofluorescence, Proc. Natl. Acad. Sci., 74: 738.
Habeshaw, J. A., Catley, P. F., Stansfield, A. G., Ganeshaguru, K., and Hoffbrand, A. V., 1979, Terminal deoxynucleotidyl transferase activity in lymphoma, Br. J. Cancer, 39: 566.
Hoffbrand, A. V., Ganeshaguru, K., Janossy, G., Greaves, M. F., Catowsky, D., and Woodruff, R. K., 1977, Terminal deoxynucleotidyl transferase levels and membrane phenotypes in diagnosis of acute leukemia. Lancet, 2: 520.
Hoffbrand, A. V., Ganeshaguru, K., Llewelin, P., and Janossy, G,1979, in: “Biochemical markers in leukemia and lymphoma,” p. 25, R. Gross and Hellriegel K. P., eds., Springer Verlag, Berlin.
Hoffbrand, A. V., and Janossy, G., 1981, Enzyme and membrane markers in leukemia: recent developments, J. Clin. Pathol., 34: 254.
Hutton, J. J., Coleman, M. S., Keneklis, T. P., and Bollum, F. J., 1979, Terminal deoxynucleotidyl transferase as a tumor cell marker in leukemia and lymphoma: results from ICXX) patients. Adv. Med. Oncol. Res, and Educ., 4: 165.
Kato, K., Goncalves, J. M., Houts, G. E., and Bollum, F. J., 1967, Deoxynucleotide-polymerizing enzymes of calf thymus gland. II. Properties of the terminal deoxynucleotidyl transferase, J. Biol. Chem., 242, 2780.
Koziner, B., Filippa, D. A., Mertelsmann, R., Gupta, S., Clarkson, B., Good, R. A., and Siegal, F. P., 1977, Characterization of malignant lymphomas in leukemic phase by multiple differentiation markers of mononuclear cells. Correlation with clinical features and conventional morphology. Am. J. Med., 63: 556.
Krakow, J. S., Coutsogeorgopoulos, C, and Canellakis, E. S., 1962, Studies on the incorporation of deoxyribonucleotides and ribonucleotides into deoxyribonucleic acid, Biochim, Biophys, Acta, 55: 639.
Kung, P. C., Silverstone, A. E., McCaffrey, R. P., and Baltimore, D., 1975/ Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone, J. Exp. Med., 141: 855.
Kung, P. C., Long, J. C., McCaffrey, R. P., Ratliff, R. L., Harrison, T., A., and Baltimore, D., 1978, Terminal deoxynucleotidyl transferase in the diagnosis of leukemia and malignant limphoma. Am. J. Med., 64: 788.
Marks, S. M., Baltimore, D., and McCaffrey, R. P., 1978, Terminal deoxynucleotidyl transferase as a predictor of initial responsiveness to vincristine and prednisone in blastic chronic myelogenous leukemia. New Engl. J. Med., 298: 812.
McCaffrey, R. P., Smoler, D. F., and Baltimore, D., 1973, Terminal deoxynucleotidyl transferase in a case of childhood acute lymphoblastic leukemia, Proc. Natl. Acad. Sci., 70: 521.
McCaffrey, R. P., Harrison, T. A., Parkman, R., and Baltimore, D., 1975, Terminal deoxynucleotidyl transferase activity in human leukemic cells and in normal human thymocytes. New Engl. J. Med., 292: 775.
Ratliff, R. L., Hoard, D. E., Ott, D. G., and Hayes, F. N., 1967, Heteropolynucleotide synthesis with terminal deoxynucleotidyl transferase. Biochemistry, 6: 851.
Thiel, E., Rodt, H., Huhn, D., Netzel, B., Grosse-Wilde, H., Gane- shaguru, K., and Thierfelder, S., 1981, Multimarker classification of acute lymphoblastic leukemia: evidence for further T subgroups and evaluation of their clinical significance. Blood, 56: 759.
Vezzoni, P., Campagnari, F., Di Fronzo, G., and Clerici, L., 1981, Terminal deoxynucleotidyl transferase in human lymphomas: possible existence of forms with high and low molecular weights, Br. J. Cancer, 43: 312.
Yoneda, M., and Bollum, F. J., 1965, Deoxynucleotide-polymerizing enzymes of calf thymus gland. I. Large scale purification of terminal and replicative deoxynucleotidyl transferases, J. Biol. Chem., 240: 3385.
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© 1982 Plenum Press, New York
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Campagnari, F. et al. (1982). Studies of Terminal Deoxynucleotidyl Transferase in Normal and Neoplastic Human Cells. In: Bertazzoni, U., Bollum, F.J. (eds) Terminal Transferase in Immunobiology and Leukemia. Advances in Experimental Medicine and Biology, vol 145. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8929-3_25
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DOI: https://doi.org/10.1007/978-1-4684-8929-3_25
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