Abstract
Understanding of microbial ecology has been expanded by means of isolation, cultivation, and characterization of microorganisms found in natural environments. However, there are still many bacteria which are metabolically active but remain in the so-called “nonculturable” state in nature (44). This state was originally recognized because of the difference observed between direct viable counts and conventional viable counts in seawater (17). Since then, ecological and practical importance of viable but nonculturable cells have been investigated and debated with particular emphasis on Vibrio cholerae and other gram-negative marine bacteria (3, 4, 14, 20, 43, 44). The general features of cells entering the nonculturable state can be summarized as reduction in size (27), decrease in macromolecular synthesis (28), and changes in composition of the cell wall and/or membrane (20, 26). However, little has been investigated with respect to the changes in bioenergetic state.
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References
Atsumi, T., L. McCarter, and Y. Imae. 1992. Polar and lateral flagellar motors of marine Vibrio are driven by different ion-motive forces. Nature 355:182–184.
Baumann, P., L. Baumann, M. Woolkalis, and S. Bang. 1983. Evolutionary relationships in Vibrio and Photobacterium: a basis for a natural classification. Annu. Rev. Microbiol. 37:369–398.
Colwell, R. R., P. R. Brayton, D. J. Grimes, D. B. Roszak, S. A. Huq, and L. M. Palmer. 1985. Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for the release of genetically engineered microorganisms. Bio/Technology 3:817–820.
Colwell, R. R., M. L. Tamplin, P. R. Brayton, A. L. Gauzens, B. D. Tall, D. Herrington, M. M. Levine, S. Hall, A. Huq, and D. A. Sack. 1990. Environmental aspects of Vibrio cholerae in transmission of cholera, p. 327-343. In R. B. Sack and Y. Zinnaka (ed.), Advances on Cholera and Related Diarrheas, vol. 7. KTK Scientific, Tokyo, Japan.
Distel, D. L. 1998. Evolution of chemoautotrophic endosymbioses in bivalves. BioScience 48:277–286.
Dunlap, P. V. 1984. Physiological and morphological state of the symbiotic bacteria from light organ of ponyfish. Biol. Bull. 176:410–425.
Dunlap, P. V. 1985. Osmotic control of luminescence and growth in Photobacterium leiognathi from ponyfish light organs. Arch. Microbiol. 141:44–50.
Dunlap, P. V. 1989. Regulation of luminescence by cyclic AMP in cya-like and crp-like mutants of Vibrio fischeri. J. Bacteriol. 171:1199–1202.
Grogan, D. W. 1984. Interaction of respiration and luminescence in a common marine bacterium. Arch. Microbiol. 137:159–162.
Hastings, J. W., and K. H. Nealson. 1977. Bacterial bioluminescence. Annu. Rev. Microbiol. 31: 549-595.
Hastings, J. W., J. C. Makemson, and P. V. Dunlap. 1987. How are growth and luminescence regulated independently in light organ symbionts? Symbiosis 4:3–24.
Haygood, M. G., and K. H. Nealson. 1985. The effect of iron on the growth and luminescence of the symbiotic bacterium Vibrio fischeri. Symbiosis 1:39–51.
Haygood, M. G., and D. L. Distel. 1993. Bioluminescent symbionts of flashlight fishes and deepsea anglerfishes form unique lineages related to the genus Vibrio. Nature 363:154–156.
Hoff, K. A. 1989. Survival of Vibrio anguillarum and Vibrio salmonicida at different salinities. Appl. Environ. Microbiol. 55:1775–1786.
Imae, Y., and T. Atsumi. 1989. Na+-driven bacterial flagellar motors. J. Bioenerg. Biomembr. 21: 705-716.
Jannasch, H. W., and D. C. Nelson. 1984. Recent progress in the microbiology of hydrothermal vents, p. 170-176. In M. J. King and C. A. Reddy (ed.), Current Perspectives in Microbial Ecology. American Society for Microbiology, Washington, D.C.
Kogure, K., U. Simidu, and N. Taga. 1979. A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol. 25:415–420.
Kogure, K. 1998. Bioenergetics of marine bacteria. Curr. Opin. Biotechnol. 9:278–282.
Lee, K-H., and E. G. Ruby. 1995. Symbiotic role of the viable but nonculturable state of Vibrio fischeri in Hawaiian coastal seawater. Appl. Environ. Microbiol. 61:278–283.
Linder, K., and J. D. Oliver. 1989. Membrane fatty acid and virulence changes in the viable but nonculturable state of Vibrio vulnificus. Appl. Environ. Microbiol. 55:2837–2842.
Makemson, J. C. 1986. Luciferase-dependent oxygen consumption by bioluminescent vibrios. J. Bacteriol. 165:461–466.
Makemson, J. C., and J. W. Hastings. 1986. Luciferase-dependent growth of cytochrome-deficient Vibrio harveyi. FEMS Microbiol. Ecol. 38:79–85.
Mitchell, P. 1961. Coupling of phosphorylation to electron and hydrogen transfer by a chemosmotic type of mechanism. Nature 191:144–148.
Nealson, K. H., T. Platt, and J. W. Hastings. 1970. The cellular control of the synthesis and activity of the bacterial luminescent system. J. Bacteriol. 104:313–322.
Nealson, K. H., and J. W. Hastings. 1977. Low oxygen is optimal for luciferase synthesis in some bacteria: ecological implications. Arch. Microbiol. 112:9–16.
Oliver, J. D. 1993. Formation of viable but nonculturable cells, p. 239-272. In S. Kjelleberg (ed.), Starvation in Bacteria. Plenum, New York, N.Y.
Rollins, D. M., and R. R. Colwell. 1986. Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment. Appl. Environ. Microbiol. 52:531–538.
Roth, W. G., M. P. Leckie, and D. N. Dietzler. 1988. Restoration of colony-forming activity in osmotically stressed Escherichia coli by betain. Appl. Environ. Microbiol. 54:3142–3146.
Ruby, E. G., and K. H. Nealson. 1976. Symbiotic association of Photobacterium fischeri with the marine luminous fish Monocentris japonica: a model of symbiosis based on bacterial studies. Biol. Bull. 151:574–586.
Ruby, E. G., and L. M. Asato. 1993. Growth and flagellation of Vibrio fischeri during initiation of the sepiolid squid light organ symbiosis. Arch. Microbiol. 159:160–167.
Ruby, E. G., and M. J. McFall-Ngai. 1992. A squid that glows in the light: development of an animal-bacterial mutualism. J. Bacteriol. 174:4865–4870.
Singleton, F. L., R. Attwell, S. Jangi, and R. R. Colwell. 1982. Effects of temperature and salinity on Vibrio cholerae growth. Appl. Environ. Microbiol. 44:1047–1058.
Tokuda, H. 1983. Isolation of Vibrio alginolyticus mutants defective in the respiration-coupled Na+ pump. Biochem. Biophys. Res. Commun. 114:113–118.
Tokuda, H., T. Nakamura, and T. Unemoto. 1981. Potassium ion is required for the generation of pH-dependent membrane potential and ApH by the marine bacterium Vibrio alginolyticus. Biochemistry 20:4198–4203.
Tokuda, H., M. Sugasawa, and T. Unemoto. 1982. Role of Na+ and K+ in α-aminoisobutyric acid transport by the marine bacterium Vibrio alginolyticus. J. Biol. Chem. 257:788–794.
Tokuda, H., and T. Unemoto. 1981. A respiration-dependent primary sodium extrusion system functioning at alkaline pH in the marine bacterium Vibrio alginolyticus. Biochem. Biophys. Res. Commun. 102:256–271.
Tokuda, H., and T. Unemoto. 1985. The Na+-motive respiratory chain of marine bacteria. Microbiol. Sci. 2:65–71.
Ulitzur, S., A. Reinhertz, and J. W. Hastings. 1981. Factors affecting the cellular expression of bacterial luciferase. Arch. Microbiol. 137:159–162.
Wada, M., K. Kogure, K. Ohwada, and U. Simidu. 1992. Coupling between the respiratory chain and the luminescent system of Vibrio harveyi. J. Gen. Microbiol. 138:1607–1611.
Wada, M., H. Tokuda, K. Kogure, and K. Ohwada. 1994. The membrane fraction of Vibrio harveyi as a possible site of in vivo luminescence, p. 560-563. In A. K. Campbell, L. J. Kricka, and P. E. Stanley (ed.), Bioluminescence and Chemiluminescence: Fundamentals and Applied Aspects. John Wiley & Sons, Chichester, United Kingdom.
Wada, M., and P. V. Dunlap. 1997. Molecular cloning of the respiratory NADH dehydrogenase (NDH-2) from Vibrio fischeri, abstr. I-60, p. 331. In Abstracts of the 97th General Meeting of the American Society for Microbiology 1997. American Society for Microbiology, Washington, D.C.
Watanabe, T., N. Mimura, A. Takimoto, and T. Nakamura. 1975. Luminescence and respiratory activities of Photobacterium phosphoreum. J. Biochem. 77:1147–1155.
Wolf, P. W., and J. D. Oliver. 1992. Temperature effects on the viable but nonculturable state of Vibrio vulnificus. FEMS Microbiol. Ecol. 101:33–39.
Xu, H.S., N. Roberts, F. L. Singleton, R. W. Attwell, D. J. Grimes, and R. R. Colwell. 1982. Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb. Ecol. 8:313–323.
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Wada, M., Kogure, K. (2000). Membrane Bioenergetics in Reference to Marine Bacterial Culturability. In: Colwell, R.R., Grimes, D.J. (eds) Nonculturable Microorganisms in the Environment. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0271-2_4
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DOI: https://doi.org/10.1007/978-1-4757-0271-2_4
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