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Culturing Interstitial Stem Cells in Hydra Aggregates

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Hydra: Research Methods

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Abstract

To investigate the growth and differentiation of interstitial stem cells of hydra, we have developed a method for culturing those cells within aggregates of cells taken from hydra previously treated with nitrogen mustard (NM) (David and Murphy, 1977; Sproull and David, 1979).

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References

  • David, C. N., and Murphy, S. 1977. Characterization of interstitial stem cells in hydra by cloning. Dev. Biol. 58: 372–383.

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  • David, C. N., and Plotnick, I. 1980. Distribution of interstitial stem cells in Hydra. Dev. Biol. 76: 175–184.

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  • Diehl, F., and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of hydra. 1. Specific destruction of interstititial cells in normal, sexual and non-budding animals./. Exp. Zoo1.155:253–259.

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  • Fujisawa, T., and David, C. N. 1981. Commitment during nematocyte differentiation in hydra. J. Cell Sci. 48:207–222

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  • Gierer, A., Berking, S., Bode, H., David, C. N., Flick, K., Hansmann, G., Schaller, C., and Trenker, E. 1972. Regeneration of hydra from reaggregated cells. Nature (London) New Biol. 239: 98–101.

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  • Sproull, R, and David, C. N. 1979. Stem cell growth and differentiation in Hydra attenuata. I, Regulation of self-renewal probability in multi-clone aggregates. J. Cell Sei. 38. 155–169.

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  • Venugopal, G., and David, C. N. 1981. Nerve commitment in hydra. I. Role of morphogenetic signals. Der. Biol. 83: 353–360.

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  • Yaross, M., Baca, B., and Bode, H. 1982. Commitment of hydra interstitial cells to nerve cell differentiation must be initiated during S-phase. Der. Biol. (in press).

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Author information

Authors and Affiliations

  1. Department of Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, USA

    Charles N. David

Authors
  1. Charles N. David
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Editor information

Editors and Affiliations

  1. Department of Developmental and Cell Biology, University of California, 92717, Irvine, California, USA

    Howard M. Lenhoff

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© 1983 Springer Science+Business Media New York

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David, C.N. (1983). Culturing Interstitial Stem Cells in Hydra Aggregates. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_34

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  • DOI: https://doi.org/10.1007/978-1-4757-0596-6_34

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0598-0

  • Online ISBN: 978-1-4757-0596-6

  • eBook Packages: Springer Book Archive

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