Abstract
Membrane proteins are chemically much the same as soluble proteins, with a similar amino acid profile, although they do contain, on average, a slightly higher proportion of nonpolar amino acids. Following cell disruption in a neutral, isotonic buffer that does not contain detergents, many proteins are associated with insoluble components of the cell extract. Integral membrane proteins, material trapped within an organelle, and other proteins that are strongly associated with the cytoskeletal matrix will all be found in the particulate fraction. When one considers that the cytosol is responsible for roughly 75% of cellular protein, a protein exclusively found in the precipitate will be enriched fourfold over the crude extract. This section discusses strategies for dissociating a membrane into its individual components in such a way that the protein of interest retains its bioactivity and becomes amenable to standard purification and analytical procedures. Since there exists no single procedure to characterize the different types of membrane proteins, several methods will be presented to begin this process.
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© 1996 Springer Science+Business Media New York
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Rosenberg, I.M. (1996). Membrane and Particulate-Associated Proteins. In: Protein Analysis and Purification. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4757-1108-0_6
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DOI: https://doi.org/10.1007/978-1-4757-1108-0_6
Publisher Name: Birkhäuser, Boston, MA
Print ISBN: 978-0-8176-3665-4
Online ISBN: 978-1-4757-1108-0
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