Abstract
This chapter describes the basic properties of integral membrane channels from both cardiac sarcoplasmic reticulum and sarcolemma. Channels are studied, under voltage-clamp conditions, following their incorporation into planar phospholipid bilayers by fusion of isolated native membrane vesicles with preformed membranes. The rate of fusion of vesicles may be influenced by a number of factors including divalent cations and negatively charged phospholipids in the preformed bilayer.
Mammalian cardiac sarcoplasmic reticulum contains a monovalent cation selective channel with a single-channel conductance of approximately 150 pico-Siemens in the presence of symmetrical solutions of 500 mM K+ at holding potentials ranging from -60 to +60 mV. The probability of the channel being in the open state is high at positive holding potentials and low at negative holding potentials.
Mammalian cardiac sarcolemma contains at least three K+-selective channels and one Cl--selective channel.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Junge, D. 1981. Nerve and Muscle Excitation, 2nd ed. Sinauer Associates, Sunderland, Massachusetts.
Noble, D. 1979. The Initiation of the Heart Beat, 2nd ed. Clarendon Press. Oxford.
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfluegers Arch. 391:85–100.
Reuter, H., Stevens, C. F., Tsien, R. W., and Yellen, G. 1982. Properties of single calcium channels in cardiac cell culture. Nature (London) 297:501–504.
Colquhoun, D., Neher, E., Reuter, H., and Stevens, C. F. 1981. Inward current channels activated by intracellular calcium in cultured cardiac cells. Nature (London) 294:752–754.
Miller, C. 1978. Voltage-gated cation conductance channel from sarcoplasmic reticulum: Steady-state electrical properties. J. Membr. Biol. 40:1–23.
Coronado, R., and Miller, C. 1979. Voltage-dependent caesium blockade of a cation channel from fragmented sarcoplasmic reticulum. Nature (London) 280:807–810.
Labarca, P., Coronado, R., and Miller, C. 1980. Thermodynamic and kinetic studies of the gating behavior of a K+-selective channel from the sarcoplasmic reticulum membrane. J. Gen. Physiol. 76:397–424.
Coronado, R., Rosenberg, R. L., and Miller, C. 1980. Ionic selectivity saturation and block in a K+ channel from sarcoplasmic reticulum. J. Gen. Physiol. 76:425–446.
Coronado, R., and Miller, C. 1982. Conduction and block by organic cations in a K+-selective channel from sarcoplasmic reticulum incorporated into planar phospholipid bilayers. J. Gen. Physiol. 79:529–547.
Miller, C. 1982. Bis-quaternary ammonium blockers as structural probes of the sarcoplasmic reticulum K+ channel. J. Gen. Physiol. 79:869–891.
White, M. M., and Miller, C. 1981. Probes of the conduction process of a voltage gated Cl channel from Torpedo electroplax. J. Gen. Physiol. 78:1–18.
Miller, C. 1982. Open-state substructure of single chloride channels from Torpedo electroplax. Philos. Trans.R.Soc. Lond. Ser. B 299:401–411.
Latorre, R., Vergara, C., and Hidalgo, C. 1982. Reconstitution in planar lipid bilayers of a Ca2+-dependent K+ channel from transverse tubule membrane isolated from rabbit skeletal muscle. Proc. Natl. Acad. Sci. U.S.A. 79:805–809.
Krueger, B. C., Worley, J. F., and French, R. J. 1983. Single sodium channels from rat brain incorporated into planar lipid bilayer membranes. Nature (London) 303:172–175.
Jones, L. R., Besch, H. R., Fleming, J. W., McConnaughey, M. M., and Watanabe, A.M. 1979. Separation of vesicles of cardiac sarcolemma from vesicles of cardiac sarcoplasmic reticulum. J. Biol. Chem. 254:530–539.
Cohen, F. S., Zimmerberg, J., and Finkelstein, A. 1980. Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. II. Incorporation of vesicular membrane marker into the planar membrane. J. Gen. Physiol. 75:251–270.
Meissner, G., and McKinley, D. 1982. Permeability of canine cardiac sarcoplasmic reticulum vesicles to K+, Na+, H+ and Cl-. J. Biol. Chem. 257:7704–7711.
Coronado, R., and Latorre, R. 1982. Detection of K+ and Cl- channels from calf cardiac sarcolemma in planar lipid bilayer membranes. Nature (London) 298:849–852.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Springer Science+Business Media New York
About this chapter
Cite this chapter
Williams, A.J. (1985). The Measurement of Cardiac Membrane Channels following Their Incorporation into Phospholipid Bilayers. In: Harris, P., Poole-Wilson, P.A. (eds) Advances in Myocardiology. Advances in Myocardiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1287-2_5
Download citation
DOI: https://doi.org/10.1007/978-1-4757-1287-2_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-1289-6
Online ISBN: 978-1-4757-1287-2
eBook Packages: Springer Book Archive