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Mapping and Measuring Surfaces Using Reflection Confocal Microscopy

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Handbook of Biological Confocal Microscopy

Abstract

Even though today biological confocal microscopy is mainly performed using the fluorescence mode, the first biomedical applications used reflection microscopy of either fixed and stained brain (Minsky, 1988) or living brain (Petráň et al., 1968; Boyde, 1994). Many areas of special biological interest still involve the recording and analysis of confocal images of reflective interfaces. Within this field, one can identify an even narrower one in which the aim is to record the three-dimensional (3D) shape of a surface with exactitude. To date, this has usually implied that the biological object is dead or dormant, because it takes time to obtain a 3D data set, and the image will be spoiled if the object moves or changes shape during that time. Now, however, fast disk-scanning TSMs with flat spectrum sources and special objective lenses with linear chromatic dispersion (LCD) can provide 3D data in which height is coded in color, at video rate.

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Authors and Affiliations

  1. Department of Anatomy and Developmental Biology, University College London, London, WC1E 6BT, UK

    Alan Boyde & Sheila J. Jones

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  1. Alan Boyde
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  2. Sheila J. Jones
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Editors and Affiliations

  1. Zoology Department, University of Wisconsin-Madison, Madison, Wisconsin, USA

    James B. Pawley

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© 1995 Springer Science+Business Media New York

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Boyde, A., Jones, S.J. (1995). Mapping and Measuring Surfaces Using Reflection Confocal Microscopy. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5348-6_15

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  • DOI: https://doi.org/10.1007/978-1-4757-5348-6_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-5350-9

  • Online ISBN: 978-1-4757-5348-6

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