Abstract
A sample preparation method was developed that facilitates imaging of powders by force microscopy. The feasibility of the method was tested by imaging a NaC1 sample prepared in a like manner. The characteristic face-centered cubic (fcc) structure common to the sodium halide salts was apparent in NaC1 atomic scale images. A periodicity of 0.60 nm, which is within 6% of the value of the NaC1 lattice constant, was measured.
By suspending biomineral powders in ethanol and depositing an aliquot onto a suitable substrate, the images of hydroxyapatite ([Ca10(PO4)6(OH)2]) and brushite ([CaHPO4. 2H2O]) were obtained. On glass substrates, brushite formed flat platelets. Atomic spacings were found to be 0.45 nm and 0.60 nm, which agree well with published values for its <110> crystal plane. In contrast, hydroxyapatite primarily formed well-isolated clusters with atomic spacings of 0.43 nm and 0.68 nm, in agreement with published values for its <110> and <001> crystal planes, respectively.
Preliminary results indicate that substrates affect the structure of the deposits. Structural differences of the two mineral deposits on glass and mica were observed.
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Siperko, L.M., Landis, W.J. (1994). Atomic Force Microscopic Imaging of Biomineral Powder Samples Formed by Deposits from Ethanolic Suspensions. In: Cohen, S.H., Bray, M.T., Lightbody, M.L. (eds) Atomic Force Microscopy/Scanning Tunneling Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9322-2_10
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DOI: https://doi.org/10.1007/978-1-4757-9322-2_10
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