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Methods for Measuring Chloride Transport across Nerve, Muscle, and Glial Cells

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Chloride Channels and Carriers in Nerve, Muscle, and Glial Cells

Abstract

Intracellular Cl− together with HCO −3 is the most abundant free anion in living cells. Measuring intracellular chloride activity (a iCl ) and studying the mechanisms involved in regulation of intracellular Cl− is particularly important in excitable cells for four main reasons: (1) a iCl is a quantity needed to determine E Cl, the Cl− equilibrium potential. (2) Several transport mechanisms responsible for intracellular pH regulation are tightly coupled to Cl−. (3) Cl− is also involved in transport mechanisms implicated in cell volume regulation. (4) Knowledge of intracellular Cl− homeostasis is crucial for understanding synaptic inhibition.

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Authors and Affiliations

  1. Departamento de Farmacología y Toxicología, Centro de Investigacíon y de Estudios Avanzados deo I. P. N., Mexico D. F., 07000, Mexico

    Francisco J. Alvarez-Leefmans

  2. Departamento de Neurobiología, Instituto Mexicano de Psiquitría, Mexico D. F., 14370, Mexico

    Fernando Giraldez

  3. Departamento de Bioquimica, Biología Molecular y Fisiología, Universidad de Valladolid, 47005, Valladolid, Spain

    John M. Russell

  4. Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas, 77550, USA

    Francisco J. Alvarez-Leefmans

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  1. Francisco J. Alvarez-Leefmans
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  1. Departamento de Farmacología y Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, 07000, Mexico, D.F., USA

    Francisco J. Alvarez-Leefmans

  2. Departamento de Neurobiología, Instituto Mexicano de Psiquiatría, 14370, Mexico, D.F., USA

    Francisco J. Alvarez-Leefmans

  3. Department of Physiology and Biophysics, University of Texas Medical Branch, 77550, Galveston, Texas, USA

    John M. Russell

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Alvarez-Leefmans, F.J., Giraldez, F., Russell, J.M. (1990). Methods for Measuring Chloride Transport across Nerve, Muscle, and Glial Cells. In: Alvarez-Leefmans, F.J., Russell, J.M. (eds) Chloride Channels and Carriers in Nerve, Muscle, and Glial Cells. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9685-8_1

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