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Cytochrome P-450 Reductase and Cytochrome b5 in Cytochrome P-450 Catalysis

  • Chapter
Cytochrome P-450

Abstract

As the readers of this monograph should be aware, the monooxygenase reaction catalyzed by cytochrome P-450 requires the input of two electrons1,2:

EquationSource% MathType!MTEF!2!1!+- % feaagCart1ev2aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn % hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr % 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9 % vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x % fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaaeyqaiaabI % eacaqGRaGaae4tamaaBaaaleaacaqGYaaabeaakiabgUcaRiaaikda % caWGLbWaaWbaaSqabeaacqGHsislaaGccqGHRaWkcaaIYaGaaeisam % aaCaaaleqabaGaae4kaaaakiabgkziUkaabgeacaqGpbGaaeisaiaa % bUcacaqGibWaaSbaaSqaaiaabkdaaeqaaOGaae4taaaa!486C!]]</EquationSource><EquationSource Format="TEX"><![CDATA[$$ {\text{AH + }}{{\text{O}}_{\text{2}}} + 2{e^ - } + 2{{\text{H}}^{\text{ + }}} \to {\text{AOH + }}{{\text{H}}_{\text{2}}}{\text{O}} $$
(1)

In mammalian systems these two electrons are derived from NADPH; in the soluble system isolated from the bacterium Pseudomonas putida, NADH is used as the external source of electrons. A schematic representation of the two different types of electron transfer chains which deliver electrons from the reduced pyridine nucleotides to P-450 is shown in Fig. 1. The system, here referred to as Type I, is found embedded in the membranous endoplasmic reticulum of most eukaryotic cell types, while the second general class, Type II, is found in mitochondria and bacteria. The most completely described P-450 system, which is not membrane-bound, is associated with camphor metabolism in P. putida.3 The Type I electron transport chain is composed of a complex flavoprotein which has both FAD and FMN as prosthetic groups.4 FAD serves as the initial electron acceptor from NADPH, while the FMN serves to reduce the P-450.5 Later in this review, we will discuss the involvement of cytochrome b 5 as a possible component of this electron transport chain. In Type II systems, the reduced pyridine nucleotide first reduces an FAD-containing reductase which subsequently transfers electrons one at a time to a 2Fe,2S iron—sulfur protein.

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Authors and Affiliations

  1. Department of Biochemistry, University of Texas Health Sciences Center, Dallas, Texas, 75235, USA

    Julian A. Peterson & Russell A. Prough

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  1. Julian A. Peterson
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  2. Russell A. Prough
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  1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, 94143, San Francisco, California, USA

    Paul R. Ortiz de Montellano

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Peterson, J.A., Prough, R.A. (1986). Cytochrome P-450 Reductase and Cytochrome b5 in Cytochrome P-450 Catalysis. In: de Montellano, P.R.O. (eds) Cytochrome P-450. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9939-2_4

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  • DOI: https://doi.org/10.1007/978-1-4757-9939-2_4

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