Abstract
Routine pathological diagnosis of hepatic copper (Cu) has traditionally depended upon histochemical stains such as rubeanic acid, rhodanine and orcein (1). The sensitivity and specificity of these stains are often unknown. In recent years, metallothionein (MT) has been used in immunohistochemical techniques, to indicate presence and distribution of heavy metals, in particular Cu, within biological tissues (2,3). Metallothionein (MT) is a low molecular weight (6000 D), cysteine-rich, cytoplasmic protein, with a high affinity for cadmium (Cd), Cu, zinc (Zn) and other heavy metals. It is thought to be involved in cellular detoxification due to metal sequestration (4). The purpose of this study was to compare MT immunostaining with rubeanic acid staining of Cu in normal and Cu-loaded rat tissues.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
S. Goldfischer, H. Popper and I. Sternlieb, Am. J. Pathol. 99, 715–730 (1980).
L.M. Williams, H. Cunningham, A. Ghaffar, G.I. Riddoch, I. Bremner, Toxicology 55, 307–316(1989).
W.E. Evering, S. Haywood, M.E. Elmes, B. Jasani and J. Trafford, J. Pathol. 160, 305–312 (1990).
D.H. Hamer, Ann. Rev. Biochem. 55, 913–951 (1986).
P.E. Olsson and C. Hogstrand, J. Chromatogr. 402, 293–299 (1987).
J.S. Garvey, R.J. Vander Mallie and C.C. Chang, Methods Enzymol. 84, 121–138 (1982).
I.I. Uzman, Lab. Invest. 5, 229–305 (1956).
I.C. Fuentealba, S. Haywood and J. Trafford, J. Comp. Pathol. 100, 1–11 (1989).
M.E. Elmes, J.P. Clarkson, N.J. Mahy and B. Jasani, J. Pathol. 158, 131–137 (1989).
A.G.E. Pearse, in Histochemistry, Theoretical and Applied, vol. 2, A.G.E. Pearse, ed., Churchill Livingstone, Edinburgh, U.K., pp. 991–995 (1985).
R.G. Thurman, F.C. Kauffrnan and K. Jugermann, Regulation of hepatic metabolism: Intra and intercellular compartmentation, Plenum Press, New York (1986).
W.E. Evering, PhD thesis, University of Liverpool, UK (1989).
S. Haywood, J. Pathol. 145, 149–158 (1985).
S.R. Gooneratne, J. M.C.C. Howell and E. Aughey, J. Comp. Pathol. 96, 593–612 (1986).
CD. Fuller, M.E. Elmes and B. Jasani, J. Pathol. 161, 167–172 (1990).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Mullins, J.E., Fredrickson, R.A., Fuentealba, I.C., Markham, R.J.F. (1996). Comparison of an Immunohistochemical and a Histochemical Stain in Detection of Copper in Rat Tissues. In: Nève, J., Chappuis, P., Lamand, M. (eds) Therapeutic Uses of Trace Elements. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0167-5_46
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0167-5_46
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0169-9
Online ISBN: 978-1-4899-0167-5
eBook Packages: Springer Book Archive