Abstract
Hepatitis B virus (HBV) is a particle of 42 nm diameter (the Dane particle) consisting of a core containing the viral genome (3200 base pairs of partially single-stranded DNA (1)) bound to the core protein and its own DNA polymerase (2,3); the virus core is surrounded by a phospholipid-containing envelope carrying the major surface antigenic determinants (HBsAg). These reside in a single protein, which occurs in both a glycosylated and non-glycosylated form of 27,000 and 23,000 daltons respectively (4,5). Infection with hepatitis B virus leads not only to the production of Dane particles but also to a dramatic overproduction of 22 nm large particles and filaments (the HBsAg particle) that contain the elements of the surface envelope. These HBsAg particles are about 1000-fold more immunogenic than the unassembled HBsAg protein (6). Core particles produced by removal of the surface coat protein contain a major polypeptide (the core antigen, HBcAg) of MW 19,000. This protein is very basic and interacts with the viral DNA in the center of the viral particle.
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Valenzuela, P. et al. (1984). Synthesis and Assembly of Hepatitis B Virus Antigens in Heterologous Systems. In: Millman, I., Eisenstein, T.K., Blumberg, B.S. (eds) Hepatitis B. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0369-3_18
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DOI: https://doi.org/10.1007/978-1-4899-0369-3_18
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