Abstract
We have recently demonstrated that light-induced Fourier transform infrared (FTIR) spectroscopy can be applied to bacterial reaction centers -RCs- (1–3) as well as to photosystems from green plants (4) in order to detect changes in the molecular interactions between the pigments involved in the primary charge separation and their anchoring sites in the protein. Using FTIR difference spectroscopy the sensitivity is high enough to detect perturbations in the vibrational modes of chlorophylls and protein groups in a large complex such as a RC. For both primary electron donor photooxidation and intermediary electron acceptor photoreduction we have reported very specific absorbance changes (1–4). However, due to the non selectivity of IR spectroscopy, the FTIR signals might arise from the pigments, the protein (peptide and side chains groups), the lipids and even bound water molecules. Thus, a precise assignment of the FTIR absorbance changes to chemical bonds requires further investigations using model compounds radicals of the isolated chlorophylls as well as isotope-substituted material.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
W. Mäntele, E. Nabedryk, B. A. Tavitian, W. Kreutz and J. Breton (1985) Light-induced Fourier transform infrared (FTIR) spectroscopic investigations of the primary donor oxidation in bacterial photosynthesis. FEBS Lett. 187, 227–232.
E. Nabedryk, W. Mäntele, B. A. Tavitian and J. Breton (1986) Light-induced Fourier transform infrared spectroscopic investigations of the intermediary electron acceptor reduction in bacterial photosynthesis. Photochem. Photobiol. 43, 461–465.
E. Nabedryk, B. A. Tavitian, M. Mäntele, W. Kreutz and J. Breton (1987) Fourier transform infrared (FTIR) spectroscopic investigations of the primary reactions in purple photosynthetic bacteria. In: “Progress in Photosynthesis Research Vol. I, pp. 177–180 (Biggins, J. ed.) Martinus Nijhoff Publishers, Dordrecht.
B. A. Tavitian, E. Nabedryk, W. Mäntele and J. Breton (1986) Light-induced Fourier transform infrared (FTIR) spectroscopic investigations of primary reactions in photosystem I and photosystem II. FEBS Lett. 201, 151–157.
W. Mäntele, A. Wollenweber, E. Nabedryk, J. Breton, F. Rashwan, J. Heinze and W. Kreutz (1987) Fourier-transform infrared (FTIR) spectroelectrochemistry of bacteriochlorophylls. In: “Progress in Photosynthesis Research, Vol. I, pp 329–332 (Biggins, J. ed) Martinus Nijhoff Publishers, Dordrecht.
S. Andriariambinintsoa, A.-M. Bardin, G. Berger, A. Bourdet, J. Breton, G. Hervo and E. Nabedryk. Colloque de Photosynthèse, “Caractérisation du quantasome de Rps. viridis, Rsp. rubrum et Rps. sphaeroides”, Saclay 24–25 April 1986.
A. Wollenweber (1986) Diplomarbeit Universität Freiburg, FRG, “Spektroelektrochemische Untersuchungen an den Bakteriochlorophyllen a und b, sowie den Bakteriophäophytinen a und b”.
W. Mäntele, A. Wollenweber, F. Rashwan, J. Heinze, E. Nabedryk, G. Berger and J. Breton (1987) Fourier transform infrared spectroelectrochemistry of the bacteriochlorophyll a anion radical. Photochem. Photobiol., in press.
M. S. Davis, A. Forman, L. K. Hanson, J. P. Thornber and J. Fajer (1979) Anion and cation radicals of bacteriochlorophyll and bacteriopheophytin b. Their role in the primary charge separation of Rhodopseudomonas viridis. J. Phys. Chem. 83, 3325–3332.
K. Ballschmiter and J. J. Katz (1969) An infrared study of chlorophyll-chlorophyll and chlorophyll-water interactions. J. Am. Chem. Soc. 91, 2661–2677.
D. F. Bocian, N. J. Boldt, B. W. Chadwick and H. A. Frank (1987) Near-infrared-excitation resonance Raman spectra of bacterial photosynthetic reaction centers. Implications for path-specific electron transfer. FEBS Lett. 214, 92–96.
F. Siebert, W. Mäntele and W. Kreutz (1982) Evidence for the protonation of two internal carboxylic groups during the photocycle of bacteriorhodopsin. FEBS Lett. 141, 82–87.
H. Michel, O. Epp and J. Deisenhofer (1986) Pigment-protein interactions in the photosynthetic reaction centre from Rhodopseudomonas viridis. The Embo Journal 5, 2445–2451.
G. Feher, R.A. Isaacson, M.Y. Okamura and W. Lubitz, ENDOR of exchangeable protons of the reduced intermediate acceptor in reaction centers from Rhodobacter sphaeroides R-26, these proceedings.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer Science+Business Media New York
About this chapter
Cite this chapter
Nabedryk, E., Andrianambinintsoa, S., Mäntele, W., Breton, J. (1988). FTIR Spectroscopic Investigations of the Intermediary Electron Acceptor Photoreduction in Purple Photosynthetic Bacteria and Green Plants. In: Breton, J., Verméglio, A. (eds) The Photosynthetic Bacterial Reaction Center. NATO ASI Series, vol 149. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0815-5_25
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0815-5_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0817-9
Online ISBN: 978-1-4899-0815-5
eBook Packages: Springer Book Archive