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Structural Information on Proteins from Circular Dichroism Spectroscopy Possibilities and Limitations

  • Chapter
Physical Methods to Characterize Pharmaceutical Proteins

Part of the book series: Pharmaceutical Biotechnology ((PBIO,volume 7))

Abstract

In this chapter we give a general survey of the application of circular dichroism (CD) spectroscopy for structural studies of proteins. In addition to the commonly used far-UV and near-UV/visible CD, the less well-known vibrational CD will be discussed. Although magnetic CD (MCD) has a fundamentally different origin and is beyond the scope of this chapter, some theoretical and experimental considerations will be given in Section 2. For a detailed treatise, we refer the reader to Sutherland and Holmqvist (1980), Sharonov (1991), and Cheesman et al. (1991). Time-resolved CD is discussed briefly in Section 6.

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Authors and Affiliations

  1. Department of Protein and Molecular Biology, Royal Free Hospital School of Medicine, London, NW3 2PF, England

    Michael Bloemendal

  2. Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, 97331, USA

    W. Curtis Johnson Jr.

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  2. W. Curtis Johnson Jr.
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Editors and Affiliations

  1. University of Utah, Salt Lake City, Utah, USA

    James N. Herron

  2. National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands

    Wim Jiskoot

  3. Groningen-Utrecht Institute for Drug Exploration, Utrecht University, and Utrecht Institute for Pharmaceutical Sciences, Utrecht, The Netherlands

    Daan J. A. Crommelin

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© 1995 Springer Science+Business Media New York

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Bloemendal, M., Johnson, W.C. (1995). Structural Information on Proteins from Circular Dichroism Spectroscopy Possibilities and Limitations. In: Herron, J.N., Jiskoot, W., Crommelin, D.J.A. (eds) Physical Methods to Characterize Pharmaceutical Proteins. Pharmaceutical Biotechnology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1079-0_2

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  • DOI: https://doi.org/10.1007/978-1-4899-1079-0_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1081-3

  • Online ISBN: 978-1-4899-1079-0

  • eBook Packages: Springer Book Archive

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