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Fourier Transform Infrared Spectroscopy Investigations of Protein Structure

  • Chapter
Physical Methods to Characterize Pharmaceutical Proteins

Part of the book series: Pharmaceutical Biotechnology ((PBIO,volume 7))

Abstract

It has been recognized since 1950 (Elliott and Ambrose, 1950) that infrared spectroscopy has the potential to provide information regarding protein secondary structure. Krimm lists nine protein-sensitive infrared active vibrations arising from the amide backbone linkage (Krimm and Bandekar, 1986). The majority of protein structural information, however, has been obtained from one absorbance originating primarily from the amide C=O stretching vibrations: the amide I band. The sensitivity to variations in both geometric arrangement of atoms and hydrogen bonding enables infrared spectroscopy to discriminate between the various secondary structures, i. e., helical, extended sheet, unordered, and turns, incorporated within the three-dimensional organization of peptides and proteins. Many studies have demonstrated, both theoretically and experimentally, that infrared spectroscopy can be used to identify specific secondary structures.

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Authors and Affiliations

  1. Department of Bioengineering, University of Utah, Salt Lake City, Utah, 84112, USA

    E. A. Cooper

  2. Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah, 84112, USA

    K. Knutson

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  1. E. A. Cooper
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  2. K. Knutson
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Editors and Affiliations

  1. University of Utah, Salt Lake City, Utah, USA

    James N. Herron

  2. National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands

    Wim Jiskoot

  3. Groningen-Utrecht Institute for Drug Exploration, Utrecht University, and Utrecht Institute for Pharmaceutical Sciences, Utrecht, The Netherlands

    Daan J. A. Crommelin

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© 1995 Springer Science+Business Media New York

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Cooper, E.A., Knutson, K. (1995). Fourier Transform Infrared Spectroscopy Investigations of Protein Structure. In: Herron, J.N., Jiskoot, W., Crommelin, D.J.A. (eds) Physical Methods to Characterize Pharmaceutical Proteins. Pharmaceutical Biotechnology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1079-0_3

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  • DOI: https://doi.org/10.1007/978-1-4899-1079-0_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-1081-3

  • Online ISBN: 978-1-4899-1079-0

  • eBook Packages: Springer Book Archive

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