Abstract
Slow (Nernstian, or redistribution) dyes monitor membrane potential by their voltage-sensitive partition between the extracellular medium and cytosol, which brings about changes in probe fluorescence intensity.1–3 Two different effects are generally responsible for these changes: (i) fluorescence quenching due to the aggregation of fluorochromes upon their accumulation in cells, and (ii) the appearance of a new fluorescent component which is typical of a dye fraction bound to cytosolic macromolecules and intracellular membranes.
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© 1996 Springer Science+Business Media New York
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Plášek, J., Sigler, K. (1996). Spectral Effects of Slow Dye Binding to Cells and Their Role in Membrane Potential Measurements. In: Slavík, J. (eds) Fluorescence Microscopy and Fluorescent Probes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1866-6_22
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DOI: https://doi.org/10.1007/978-1-4899-1866-6_22
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