Abstract
Heterogeneous immunoassays are in their general performance characterized by a sequence of experimental steps. In a rapid ELISA (enzyme linked immunosorbent assay) all the individual steps were initially performed manually. This resulted in a variation between assays of approximately 10%. A critical step was the binding reaction. To reduce the variation in this step, binding was allowed to come to equilibrium. The washing steps as well as the development with enzyme substrate have been automated using specially developed washing apparati. A way to improve the speed of an assay without loosing in accuracy is to expose antigen to antibody under well controlled conditions. Such a goal is achieved by applying enzyme linked immunosorbent assays in continuous flow systems. Flow systems may offer certain advantages since washing, development and reading may also be performed under well controlled conditions.
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References
Axen, R., Porath, J. and Ernback, S., 1967, Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides, Nature 214: 1302.
Becton Dickinson Inc., Broschure on ARIA.
Borrebaeck, C., Börjesson, C. and Mattiasson, B., 1978, Thermometric Enzyme Linked Immunosorbent Assay in Continuous Flow System: Optimizaton and Evaluation Using Human Serum Albumin as a Model System. Clin. Chim. Acta 86: 267.
Borrebaeck, C. and Mattiasson, B., 1980, Lectin-Carbohydrate Interactions Studied by a Competitive Enzyme Inhibition Assay. Anal. Biochem. 107: 446.
Borrebaeck, C., Mattiasson, B. and Svensson, K., 1978, A rapid non-equilibrium enzyme immunoassay for determining serum gentamicin, in: “Enzyme labelled immunoassays of hormons and drugs” S.B. Pal, ed., pp 15–27, Walter de Gruyter & Co., Berlin.
Danielsson, B., Mattiasson, B. and Mosbach, K., 1981, Enzyme Thermistor Devices and Their Analytical Applications, in: “Appl. Biochem. Bioeng.” Vol 3, pp 97–143. L.B. Wingard, Jr., E. Katchalski-Katzir and L. Goldstein, eds, Academic Press, New York.
Hornby, W.E., Campbell, J., Inman, D.J. and Morris, D.L., 1974, Preparation of immobilized enzymes for application in automated analysis, in: “Enzyme Engineering” Vol 2, pp 401–407, E.K. Pye and L. B. Wingard, Jr., ed., Plenum Press, New York.
Mattiasson, B., 1984, Immunochemical assays for process control: potentials and limitations. Trends Anal. Chem. 3: 245.
Mattiasson, B., Borrebaeck, C.A.K., Sanfridson, B. and Mosbach, K, 1977, Thermometric Enzyme Linked Immunosorbent Assay: TELISA, Biochim. Biophys. Acta 483: 221.
Mattiasson, B., Danielsson, B. and Mosbach, K., 1980, Applications of the enzyme thermistor in analysis and process control, in: “Food Process Engineering” vol 2, pp 59–68, P. Linko and J. Larinkari, eds., Appl. Sci. Publ., London.
Mattiasson, B. and Nilsson, H., 1977, An enzyme immunoelectrode, FEBS Lett. 78: 251.
Mattiasson, B., Svensson, K., Borrebaeck, C., Jonsson, S. and Kronvall, G., 1978, Non-equilibrium enzyme immunoassay of gentamicin. Clin. Chem. 24: 1770.
Ullman, E.F. and Maggio, E.T., 1980, Principles of Homogeneous Enzyme Immunoassay, in: “Enzyme-Immunoassay” pp 105–134, E.T. Maggio, ed., CRC-Press, Boca Raton, Fl. USA.
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© 1987 Springer Science+Business Media New York
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Mattiasson, B. (1987). Enzyme Immunoassay in Flow Systems with Electrochemical and other Detectors. In: Ngo, T.T. (eds) Electrochemical Sensors in Immunological Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1974-8_23
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DOI: https://doi.org/10.1007/978-1-4899-1974-8_23
Publisher Name: Springer, Boston, MA
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