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The use of Molecular Techniques for the Location and Characterisation of Antibiotic Resistance Genes in Clinical Isolates of Acinetobacter

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The Biology of Acinetobacter

Part of the book series: Federation of European Microbiological Societies Symposium Series ((FEMS,volume 57))

Abstract

Nosocomial isolates of Acinetobacter, particularly A. baumannii, are often highly resistant to a variety of antibiotics (Bergogne-Bérézin et al., 1987). Although much is known about the biochemical mechanisms of antibiotic resistance found in Acinetobacter (Bergogne-Bérézin and Joly-Guillou, this volume), little is known about the structure and regulation of the expression of these genes in Acinetobacter at the molecular level.

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References

  • Aiba, H., Adhya, S., and De Crombrugge, B., 1981, Evidence for two functional gal promotors in intact Escherichia coli, J. Biol. Chem., 256:11905.

    PubMed  CAS  Google Scholar 

  • Allmansberger, R., Brau, B., and Piepersberg, W., 1985, Genes for gentamicin-(3)-N-acetyltransferase III & IV, Mol. Gen. Genet., 198:514.

    Article  PubMed  CAS  Google Scholar 

  • Benveniste, R., and Davies, J., 1971, R-factor mediated gentamicin resistance: a new enzyme which modifies aminoglycoside antibiotics, FEBS Lett., 14:293.

    Article  PubMed  CAS  Google Scholar 

  • Bergogne-Bérézin, E., Joly-Guillou, M.L., and Vieu, J., 1987, Epidemiology of nosocomial infections due to Acinetobacter calcoaceticus, J. Hosp. Infect., 10:105.

    Article  PubMed  Google Scholar 

  • Brau, B., Pilz, U., and Piepersberg, W., 1984, Genes for gentamicin-(3)-N-acetyltransferases III & IV, Mol. Gen. Genet., 193:179.

    Article  PubMed  CAS  Google Scholar 

  • Chopade B.A., Wise, P.J., and Towner, K.J., 1985, Plasmid transfer and behaviour in Acinetobacter calcoaceticus EBF65/65, J. Gen. Microbiol., 131:2805.

    PubMed  CAS  Google Scholar 

  • Clewell, D.B., and Helinski, D.R., 1969, Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form, Proc. Natl. Acad. Sci. USA, 62:1159.

    Article  PubMed  CAS  Google Scholar 

  • Davies, J., and Smith, D., 1978, Plasmid determined resistance to antimicrobial agents, Ann. Rev. Microbiol., 32:469.

    Article  CAS  Google Scholar 

  • De Crombrugghe, B., Busby, S., and Buc, H., 1984, Cyclic AMP receptor protein: Role in transcription activation, Science, 224:831.

    Article  PubMed  Google Scholar 

  • Devaud, M., Kayser, F.H., and Bachi, B., 1982, Transposon mediated multiple antibiotic resistance in Acinetobacter strains, Antimicrob. Agents Che mother., 22:323.

    Article  CAS  Google Scholar 

  • Divers, M., Craven, P., and Vivian, A., 1985, Molecular analysis of an antibiotic resistance plasmid, pAV5, and its derivative plasmids in Acinetobacter calcoaceticus, J. Gen. Microbiol., 131:3367.

    PubMed  CAS  Google Scholar 

  • Goldstein, F.W., Labigne-Roussel, A., Gerbaud, G., Carlier, C., Collatz E., and Courvalin P., 1983, Transferable plasmid-mediated antibiotic resistance in Acinetobacter calcoaceticus, Plasmid, 10:138.

    Article  PubMed  CAS  Google Scholar 

  • Gorman, C.M., Moffat, L.F., and Harwood, B.H., 1982, Recombinant genomes which express chloramphenicol acetyltransferases in mammalian cells, Mol. Cell. Biol., 2:1044.

    PubMed  CAS  Google Scholar 

  • Harwood, J., and Smith, D., 1971, Catabolite repression of chloramphenicol acetyltransferase synthesis in E. coli K12, Biochem. Biophys. Res. Comm., 42:57.

    Article  PubMed  CAS  Google Scholar 

  • Henikoff, S., 1984, Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing, Gene, 28:351.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, D.A., Gautsch, J.W., Sportsman, J.R., and Elder, J.H., 1984, Improved technique utilizing non-fat dry milk powder for analysis of proteins and nucleic acids transferred to nitrocellulose, Gene Analyt. Tech., 1:3.

    Article  CAS  Google Scholar 

  • McClure. W.R., 1985. Mechanism and control of transcription in prokaryotes. Ann. Rev. Biochem., 54:171.

    Article  PubMed  CAS  Google Scholar 

  • Murray, B.E., and Moellering, R.C., 1980, Evidence of plasmid-mediated production of aminoglycoside modifying enzymes not previously described in Acinetobacter calcoaceticus., Antimicrob. Agents Chemother., 17:30.

    Article  PubMed  CAS  Google Scholar 

  • Prince, A.S., and Jacoby, G.A., 1982, Cloning the gentamicin resistance gene from a Pseudomonas aeruginosa plasmid in Escherichia coli enhances the detection of aminoglycoside modification, Antimicrob. Agents Chemother., 22:525.

    Article  PubMed  CAS  Google Scholar 

  • Rosenthal, N., Kress, M., Gruss, P., and Khoury, G., 1983, B.K. viral enhancer element and a human cellular homolog, Science, 222:749.

    Article  PubMed  CAS  Google Scholar 

  • Sanger, F., Nicklen, S., and Coulsen, A.R., 1977, DNA sequencing with chain terminating inhibitors, Proc. Natl. Acad. Sci. USA, 74:5463.

    Article  PubMed  CAS  Google Scholar 

  • Seth, A., 1984, A new method for linker ligation, Gene Analyt. Tech., 1:99.

    Article  CAS  Google Scholar 

  • Shannon, K.P., and Phillips, I., 1983, Detection of aminoglycoside-modifying strains of bacteria, in: “Antibiotics: Assessment of Antimicrobial Activity and Resistance,” p.183, A.D. Russell and L.B. Quesnel, eds., Academic Press, London.

    Google Scholar 

  • Shaw, W., 1983, Chloramphenicol acetyltransferase: enzymology and molecular biology, CRC Critical Rev. Biochem., 14:1.

    Article  CAS  Google Scholar 

  • Southern, E.M., 1975, Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol., 98:503.

    Article  PubMed  CAS  Google Scholar 

  • Tenover, F.C., Phillips, K.L., Gilbert, T., Lockhart, P., O’Hara, P.J., and Plorde, J., 1989, Development of a DNA probe for the deoxyribonucleotide sequence of a 3-N-acetyltransferase (AAC(3)I) resistance gene, Antimicrob. Agents Chemother., 33:551.

    Article  PubMed  CAS  Google Scholar 

  • Vivian, A., Hinchliffe E., and Fewson, C.A., 1981, Acinetobacter calcoaceticus: some approaches to a problem, J. Hosp. Infect., 2:199.

    Article  PubMed  CAS  Google Scholar 

  • Vliegenthart, J.S., Ketelaar-van Gaalen, P.A.G. and Van De Klundert, J.A.M., 1989, Nucleotide sequence of the aacC2 gene, a gentamicin resistance determinant involved in a hospital epidemic of multiply resistant members of the family Enterobacteriaceae, Antimicrob. Agents Chemother., 33:1153.

    Article  PubMed  CAS  Google Scholar 

  • Womble, D.D., and Rownd, R.H., 1988, Genetic and physical map of plasmid NRI: comparison with other IncFII antibiotic resistance plasmids, Microbiol. Rev., 52:433.

    PubMed  CAS  Google Scholar 

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© 1991 Springer Science+Business Media New York

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Elisha, B.G., Steyn, L.M. (1991). The use of Molecular Techniques for the Location and Characterisation of Antibiotic Resistance Genes in Clinical Isolates of Acinetobacter . In: Towner, K.J., Bergogne-Bérézin, E., Fewson, C.A. (eds) The Biology of Acinetobacter . Federation of European Microbiological Societies Symposium Series, vol 57. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3553-3_10

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  • DOI: https://doi.org/10.1007/978-1-4899-3553-3_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-3555-7

  • Online ISBN: 978-1-4899-3553-3

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