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Profiling and Detection of Bacterial Carbohydrates

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Analytical Microbiology Methods

Abstract

Carbohydrates are a major class of structural components in bacterial cell envelopes. Sugar profiles can differentiate and identify isolated bacteria. Carbohydrates can also serve as chemical markers for direct detection of bacteria in complex matrices such as mammalian body fluids and tissues. This chapter reviews the composition of some bacterial polysaccharides and their characteristic sugars. Preparation and analysis of derivatives of these sugar monomers by gas chromatography (GC) and mass spectrometry (MS) is described. A modified alditol acetate procedure developed in our laboratories is presented along with some examples of its use.

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Authors and Affiliations

  1. Department of Microbiology & Immunology School of Medicine, University of South Carolina, Columbia, SC, 29208, USA

    Alvin Fox & James Gilbart

  2. Department of Chemistry, University of South Carolina, Columbia, SC, 29208, USA

    Stephen L. Morgan

Authors
  1. Alvin Fox
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  2. James Gilbart
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  3. Stephen L. Morgan
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Editor information

Editors and Affiliations

  1. University of South Carolina, Columbia, South Carolina, USA

    Alvin Fox  & Stephen L. Morgan  & 

  2. University of Lund, Lund, Sweden

    Lennart Larsson  & Göran Odham  & 

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© 1990 Springer Science+Business Media New York

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Fox, A., Gilbart, J., Morgan, S.L. (1990). Profiling and Detection of Bacterial Carbohydrates. In: Fox, A., Morgan, S.L., Larsson, L., Odham, G. (eds) Analytical Microbiology Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-3564-9_5

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  • DOI: https://doi.org/10.1007/978-1-4899-3564-9_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-3566-3

  • Online ISBN: 978-1-4899-3564-9

  • eBook Packages: Springer Book Archive

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