Abstract
The next-generation sequencing field has developed at such a rapid pace that the achievements of today exceed the challenges and limitations of the last few years. We have previously described how next-generation sequencing has advanced DNA sequencing from low throughput to high throughput and minimized labor-intensive practices to more automated processes. In this chapter, we will compare next-generation sequencing with the traditional Sanger method to demonstrate the remarkable recent developments in relation to improved speed, throughput, and accuracy. Lastly, we will provide an overview of lingering challenges that are still faced by next-generation sequencing.
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References
Mardis ER (2013) Next-generation sequencing platforms. Annu Rev Anal Chem (Palo Alto Calif) 6:287-303. doi:10.1146/annurev-anchem-062012-092628
El-Metwally S, Hamza T, Zakaria M, Helmy M (2013) Next-generation sequence assembly: four stages of data processing and computational challenges. PLoS Comput Biol 9 (12):e1003345. doi:10.1371/journal.pcbi.1003345
Schuster SC (2008) Next-generation sequencing transforms today’s biology. Nat Methods 5 (1):16-18. doi:10.1038/nmeth1156
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY et al. (2010) Creation of a bacterial cell controlled by a chemically synthesized genome. Science 329 (5987):52-56. doi:10.1126/science.1190719
Collins FS, Hamburg MA (2013) First FDA authorization for next-generation sequencer. N Engl J Med 369 (25):2369-2371. doi:10.1056/NEJMp1314561
Illumina Inc. (2014) HiSeq X Ten. http://www.illumina.com/systems/hiseq-x-sequencing-system.ilmn. Accessed 17-01-2014
WHO.int (2005) Genetics, genomics and the patenting of DNA: Review of potential implications for health in developing countries. 2005. http://www.who.int/genomics/FullReport.pdf. Accessed 10-01-2014
WHO.int (2013) Wild poliovirus in the Horn of Africa – update. http://www.who.int/csr/don/2013_10_01/en/. Accessed 10-01-2014
Liu L, Li Y, Li S, Hu N, He Y et al. (2012) Comparison of next-generation sequencing systems. J Biomed Biotechnol 2012:251364. doi:10.1155/2012/251364
Quail MA, Smith M, Coupland P, Otto TD, Harris SR et al. (2012) A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers. BMC Genomics 13:341. doi:10.1186/1471-2164-13-341
Bansal V, Harismendy O, Tewhey R, Murray SS, Schork NJ et al. (2010) Accurate detection and genotyping of SNPs utilizing population sequencing data. Genome Res 20 (4):537-545. doi:10.1101/gr.100040.109
Glenn TC (2011) Field guide to next-generation DNA sequencers. Mol Ecol Resour 11 (5):759-769. doi:10.1111/j.1755-0998.2011.03024.x
Glenn TC (2013) Field guide to next-generation DNA sequencers-Update. http://www.molecularecologist.com/next-gen-fieldguide-2013/. Accessed 10-01-2014
Oyola SO, Otto TD, Gu Y, Maslen G, Manske M et al. (2012) Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes. BMC Genomics 13:1. doi:10.1186/1471-2164-13-1
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El-Metwally, S., Ouda, O.M., Helmy, M. (2014). Challenges in the Next-Generation Sequencing Field. In: Next Generation Sequencing Technologies and Challenges in Sequence Assembly. SpringerBriefs in Systems Biology, vol 7. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-0715-1_5
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DOI: https://doi.org/10.1007/978-1-4939-0715-1_5
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