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Interdependence Among Members of the mGluR6 G-protein Mediated Signalplex of Retinal Depolarizing Bipolar Cells

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G Protein Signaling Mechanisms in the Retina

Part of the book series: Springer Series in Vision Research ((SSVR,volume 3))

Abstract

Normal vision depends on signaling from photoreceptors to central visual areas via parallel pathways that are optimized for detecting increments (ON) or decrements (OFF) in light intensity. The divergence of these two pathways occurs at the first synapse. The OFF pathway is mediated via Off-bipolar cells that hyperpolarize in response to light increments because they utilize ionotropic glutamate receptors. On-bipolar cells that initiate the ON pathway utilize metabotropic glutamate receptors to signal via a G-protein cascade to the transient receptor potential melastatin 1 (TRPM1) channel, and depolarize in response to light increments. Several proteins (mGluR6, TRPM1, GPR179, RGS7, RGS11, nyctalopin, LRIT3, Gα0, Gβ3, Gβ5, and R9AP) have been shown to be required for normal functioning of the depolarizing bipolar cell cascade. Here, we use immunohistochemistry in mouse models that lack one or more of these proteins to understand their interdependency. The picture that evolves is that of a large complex, in which the removal of any one element results in either delocalization of or decreased expression of other elements.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Louisville, A Building, Rm 616, 319 Abraham Flexner Way, 40202, Louisville, KY, USA

    Ronald G. Gregg PhD, Thomas A. Ray BS, PhD & Nazarul Hasan PhD

  2. Department of Ophthalmology and Visual Sciences, University of Louisville, 301 E. Muhammad Ali Blvd, 40202, Louisville, KY, USA

    Ronald G. Gregg PhD & Maureen A. McCall PhD

  3. Anatomical Sciences and Neurobiology, University of Louisville, 40202, Louisville, KY, USA

    Maureen A. McCall PhD

  4. Cole Eye Institute, Cleveland Clinic, 9500 Euclid Avenue, 44195, Cleveland, OH, USA

    Neal S. Peachey PhD

  5. Louis Stokes Cleveland VA Medical Center, 44106, Cleveland, OH, USA

    Neal S. Peachey PhD

  6. Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, 44195, Cleveland, OH, USA

    Neal S. Peachey PhD

Authors
  1. Ronald G. Gregg PhD
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  2. Thomas A. Ray BS, PhD
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  3. Nazarul Hasan PhD
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  4. Maureen A. McCall PhD
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  5. Neal S. Peachey PhD
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Corresponding author

Correspondence to Ronald G. Gregg PhD .

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Editors and Affiliations

  1. The Scripps Research Institute, Jupiter, Florida, USA

    Kirill A. Martemyanov

  2. Jules Stein Eye Institute, University of California, Los Angeles, Los Angeles, California, USA

    Alapakkam P. Sampath

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Gregg, R., Ray, T., Hasan, N., McCall, M., Peachey, N. (2014). Interdependence Among Members of the mGluR6 G-protein Mediated Signalplex of Retinal Depolarizing Bipolar Cells. In: Martemyanov, K., Sampath, A. (eds) G Protein Signaling Mechanisms in the Retina. Springer Series in Vision Research, vol 3. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1218-6_5

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