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Asymmetrical Flow Field Flow Fractionation: A Useful Tool for the Separation of Protein Pharmaceuticals and Particulate Systems

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Analytical Techniques in the Pharmaceutical Sciences

Part of the book series: Advances in Delivery Science and Technology ((ADST))

Abstract

The focus of this chapter will be on asymmetrical flow field flow fractionation (AF4) for the separation and characterization of protein pharmaceuticals and particulate systems. The chapter will provide some background and historical information on field flow fractionation and the general working principle. In addition, a practical guide on how to use AF4 will be described and critical parameters for the development of a suitable separation method will be discussed. The use of AF4 for protein pharmaceuticals as well as particulate systems will be described and some examples given in the literature will be presented. Finally, a summary of the most recent trends in AF4 and an outlook will be given for potential application fields in the future.

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Acknowledgements

The authors wish to thank Dr.-Ing. Marcus Engert for preparing the schematic illustrations.

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Authors and Affiliations

  1. Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig-Maximilians-University Munich, Butenandtstr. 5, Munich, D-81377, Germany

    Julia Engert, Roman Mathaes & Gerhard Winter

Authors
  1. Julia Engert
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  2. Roman Mathaes
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  3. Gerhard Winter
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Corresponding author

Correspondence to Julia Engert .

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Editors and Affiliations

  1. Bioneer: FARMA Department of Pharmacy, University of Copenhagen, Copenhagen Ø, Denmark

    Anette Müllertz

  2. Strathclyde Institute of Pharmacy and Biomedical Sciences, Glasgow, Seychelles

    Yvonne Perrie

  3. Department of Pharmacy, University of Copenhagen, Copenhagen Ø, Denmark

    Thomas Rades

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Engert, J., Mathaes, R., Winter, G. (2016). Asymmetrical Flow Field Flow Fractionation: A Useful Tool for the Separation of Protein Pharmaceuticals and Particulate Systems. In: Müllertz, A., Perrie, Y., Rades, T. (eds) Analytical Techniques in the Pharmaceutical Sciences. Advances in Delivery Science and Technology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-4029-5_15

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