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Genetic Approaches to Recombinant Protein Production in Mammalian Cells

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Handbook of Industrial Cell Culture

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Abstract

Genetically engineered mammalian cells play an essential role in many processes, from basic research to high-throughput screening and pharmaceutical protein production. In basic research, mammalian cells serve to study gene function and mechanisms of regulation. Important health-related applications include drug screening and production of secreted, pharmaceutically active proteins. The reason that mammalian cells are preferred is the close relationship to cells and their products in the human body. In particular, mammalian cells have the unique capability to authentically process, fold, and modify secreted human proteins. The resulting products are free of microbial contaminants, thereby minimizing the risk of immunogenic and inflammatory responses, respectively. In addition, human-like modifications extend the in vivo lifetime of therapeutic proteins. This translates into therapeutic products that are safe and highly active.

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Authors
  1. Peter P. Mueller
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  2. Dagmar Wirth
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  3. Jacqueline Unsinger
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  4. Hansjörg Hauser
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Editors and Affiliations

  1. Eli Lilly and Company, Indianapolis, IN, USA

    Victor A. Vinci PhD

  2. Dow AgroSciences, Indianapolis, IN, USA

    Sarad R. Parekh PhD

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Mueller, P.P., Wirth, D., Unsinger, J., Hauser, H. (2003). Genetic Approaches to Recombinant Protein Production in Mammalian Cells. In: Vinci, V.A., Parekh, S.R. (eds) Handbook of Industrial Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-346-0_2

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  • DOI: https://doi.org/10.1007/978-1-59259-346-0_2

  • Publisher Name: Humana Press, Totowa, NJ

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