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Methods for Transmission and Scanning Electron Microscopy of Bone and Cartilage

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Handbook of Histology Methods for Bone and Cartilage

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Abstract

Ultrastructural studies of bone and cartilage using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) present unique challenges for technical preparation and scientific interpretation. Preparative methods have been described elsewhere; space limitations do not allow us to cite all authors who have contributed to this field. Chemical fixation and specimen preparation for TEM for bone studies have been reviewed by several authors.3,14,15,23 Reviews of preparation methods for SEM have been published by Boyde et al.7,9,10 Specialized techniques directed toward preserving the bone-cell-implant interface are discussed elsewhere in this volume.

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Author information

Authors and Affiliations

  1. Orthopaedic Research Biology, Carolinas Medical Center, Charlotte, NC, USA

    Helen E. Gruber

  2. Electron Microscopy Laboratory, Carolinas Medical Center, Charlotte, NC, USA

    Winston W. Wiggins

Authors
  1. Helen E. Gruber
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  2. Winston W. Wiggins
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Editor information

Editors and Affiliations

  1. Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston, SC, USA

    Yuehuei H. An MD (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering) (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering)

  2. Department of Bioengineering, Clemson University, Clemson, SC, USA

    Yuehuei H. An MD (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering) (Associate Professor, Director of Orthopaedic Research Laboratory, Assistant Professor of Bioengineering)

  3. Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston, SC, USA

    Kylie L. Martin BSc (Histological Technician and Laboratory Manager) (Histological Technician and Laboratory Manager)

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Gruber, H.E., Wiggins, W.W. (2003). Methods for Transmission and Scanning Electron Microscopy of Bone and Cartilage. In: An, Y.H., Martin, K.L. (eds) Handbook of Histology Methods for Bone and Cartilage. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-417-7_34

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  • DOI: https://doi.org/10.1007/978-1-59259-417-7_34

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-277-3

  • Online ISBN: 978-1-59259-417-7

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