Abstract
Reprogramming of human somatic cells represents a valuable tool for enhancing our understanding of the mechanisms underlying the acquisition of pluripotency and for realizing the potential to generate patient-specific pluripotent stem cells. Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by the ectopic expression of four transcription factors (OCT4 , SOX2, c-MYC, and KLF4). Recently, we reported that Oct4 acting singly is sufficient to directly reprogram adult mouse neural stem cells (NSCs) into iPS cells. Furthermore, we found that the generation of one-factor (1F) human iPS cells from human NSCs is possible by the ectopic expression of OCT4 alone. These human NSC-derived 1F iPS cells are indistinguishable at the molecular level from human embryonic stem cells (ESCs) and can differentiate into cells of all three germ lineages both in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram mouse and human NSCs into pluripotent cells. This chapter focuses on the generation of iPS cells from mouse or human NSCs and uses biologic concepts to compare NSCs and pluripotent stem cells such as ESCs and iPS cells.
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Kim, J.B., Zaehres, H., Schöler, H.R. (2011). Direct Reprogramming of Human Neural Stem Cells by the Single Transcription Factor OCT4. In: Appasani, K., Appasani, R. (eds) Stem Cells & Regenerative Medicine. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-860-7_27
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DOI: https://doi.org/10.1007/978-1-60761-860-7_27
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