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Molecular Detection of Resistance to Biotic Stress Conditions in Spring Bread Wheat Cultivars

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Innovative Approaches and Applications for Sustainable Rural Development (HAICTA 2017)

Part of the book series: Springer Earth System Sciences ((SPRINGEREARTH))

Abstract

Biotic stress conditions are the most serious obstacle in bread wheat cultivation resulting in yield reduction and safety problems (toxin production) for consumers. For this reason, identification of resistant cultivars and their respective genes is a main prerequisite in most breeding programs. Into this study and in order to exploit the benefits of molecular technology, 9 Hellenic and 93 Ukrainian bread wheat cultivars were analyzed to determine the responsible alleles for some important diseases in wheat. Among the Hellenic cultivars, carriers of resistance-associated alleles of the Lr34/Yr18/Pm38/Sr57/Bdv1 (a rare gene among the European bread wheat cultivars), Tsn1, Tsc2, and TDF_076_2D genes were identified. Four of the studied Hellenic cultivars carry the 1BL.1RS wheat-rye chromosome translocation of the ‘Kavkaz’ type proved to be resistant to the stem rust and especially to the dangerous Sicilian race TTTTF due mainly to the presence of the Sr31 gene. Among the Ukrainian cultivars, the toxin A insensitivity allele of the Tsn1 gene is predominant. Οf the nine Ukrainian spring cultivars containing wheat-rye translocations, seven were found to possess the 1BL.1RS and two the ‘Amigo’-type 1AL.1RS translocation. The previous two cultivars carry the gene Sr1RS Amigo conferring resistance to dangerous stem rust Ug99 biotypes. One cultivar (‘Vyshyvanka’) was found to carry a 1BL.1RS translocation distinct from 1BL.1RS of the ‘Kavkaz’ type and may carry novel rye disease resistance genes. The results of the examined germplasm regarding disease resistance gene alleles could be helpful for breeders who are looking for sources of disease resistance genes.

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Abbreviations

ATP:

Adenosine triphosphate

AFLP:

Amplified fragment length polymorphism

ABC:

ATP-binding cassette

APR:

Adult plant resistance

KASP:

Competitive allele-specific PCR

MAS:

Marker-assisted selection

NAAS:

National Academy of Agrarian Sciences

PCR:

Polymerase chain reaction

PDR:

Pleiotropic drug resistance

RFLP:

Restriction fragment length polymorphism

RAPD:

Random amplified polymorphic DNA

QTL:

Quantitative trait locus

SCAR:

Sequence characterized amplified region

SSR:

Short sequence repeat

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Authors and Affiliations

  1. Institute of Plant Protection, National Academy of Agrarian Sciences of Ukraine, Kyiv, Ukraine

    Anatolii Karelov, Natalia Kozub, Igor Sozinov & Oksana Sozinova

  2. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    Anatolii Karelov & Natalia Kozub

  3. Laboratory of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Athanasios G. Mavromatis

  4. School of Agricultural Technology & Food Technology and Nutrition, Western Macedonia University of Applied Sciences, Florina, Greece

    Ioannis N. Xynias

Authors
  1. Anatolii Karelov
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  2. Natalia Kozub
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  3. Igor Sozinov
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  4. Oksana Sozinova
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  5. Athanasios G. Mavromatis
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Correspondence to Ioannis N. Xynias .

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  1. Laboratory of Animal Production Economics, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Alexandros Theodoridis

  2. Agricultural Economics Research Institute, HAO Demeter, Athens, Greece

    Athanasios Ragkos

  3. Department of Informatics, TEI of Thessaloniki, Thessaloniki, Greece

    Michail Salampasis

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Karelov, A., Kozub, N., Sozinov, I., Sozinova, O., Mavromatis, A.G., Xynias, I.N. (2019). Molecular Detection of Resistance to Biotic Stress Conditions in Spring Bread Wheat Cultivars. In: Theodoridis, A., Ragkos, A., Salampasis, M. (eds) Innovative Approaches and Applications for Sustainable Rural Development. HAICTA 2017. Springer Earth System Sciences. Springer, Cham. https://doi.org/10.1007/978-3-030-02312-6_18

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