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Interferometric Scattering (iSCAT) Microscopy and Related Techniques

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Label-Free Super-Resolution Microscopy

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Abstract

Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive detection and imaging of nanoparticles to high spatiotemporal resolution. As it was born out of detection principles central to conventional microscopy, we begin by surveying the historical development of the microscope to examine how the exciting possibility for interferometric scattering microscopy with sensitivities sufficient to observe single molecules has become a reality. We discuss the theory of interferometric detection and also issues relevant to achieving a high detection sensitivity and speed. A showcase of numerous applications and avenues of novel research across various disciplines that iSCAT microscopy has opened up is also presented.

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Acknowledgements

We are grateful to a large number of fantastic group members, former and present, who have contributed to the advances of iSCAT in our laboratories. We also thank the Alexander von Humboldt Foundation for their generous support in the context of a Humboldt Professorship (VS) and Postdoctoral Fellowship (RWT) as well the Max Planck Society for continuous support.

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  1. Max Planck Institute for the Science of Light, 91058, Erlangen, Germany

    Richard W. Taylor & Vahid Sandoghdar

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  1. Richard W. Taylor
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Correspondence to Vahid Sandoghdar .

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  1. University of North Carolina at Charlotte, Charlotte, NC, USA

    Vasily Astratov

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Taylor, R.W., Sandoghdar, V. (2019). Interferometric Scattering (iSCAT) Microscopy and Related Techniques. In: Astratov, V. (eds) Label-Free Super-Resolution Microscopy. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-030-21722-8_2

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