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Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions

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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Part of the book series: BioMethods ((BIOMETHODS))

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Summary

Methods are described for the preparation of poly(ADP-ribose) and for the preparation of biotinylated poly(ADP-ribose). Poly(ADP-ribose) is covalently modified to its biotinylated derivative by light-induced reaction of photobiotin with the polymer. Furthermore, conditions are given for the detection of poly(ADP-ribose) binding proteins in ligand-blotting experiments. The electrophoretically separated proteins are identified on blots using biotinylated poly(ADP-ribose) in a staining reaction. The results obtained with biotinylated poly(ADP-ribose) are comparable with those obtained with radioactive poly(ADP-ribose).

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Authors
  1. Frank M. Narendja
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  2. Georg Sauermann
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Editor information

Editors and Affiliations

  1. Physiologisches Institut, Universität Basel, Vesalgasse 1, 4051, Basel, Switzerland

    Thomas Meier

  2. Max-Planck-Institut fĂĽr Biophysik, Kennedyallee 70, D-60596, Frankfurt, Germany

    Falk Fahrenholz

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© 1996 Birkhäuser Verlag

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Narendja, F.M., Sauermann, G. (1996). Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions. In: Meier, T., Fahrenholz, F. (eds) A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis. BioMethods. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7349-9_8

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  • DOI: https://doi.org/10.1007/978-3-0348-7349-9_8

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7351-2

  • Online ISBN: 978-3-0348-7349-9

  • eBook Packages: Springer Book Archive

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