Abstract
Protein glycosylation is a major post-translational modification that generates a potentially large group of glycoforms from a single polypeptide chain (1). Over 50% of catalogued protein sequences contain the requisite sequence for N-glycosylation (AsnXxxSer(Thr); Xxx ≠ Pro) (2), and any Ser or Thr can potentially be glycosylated (3). Since glycosylation can play an important role in the biological and pharmacologic activity of the glycoprotein, there is increasing interest in identifying and characterizing the oligosaccharide moieties of glycoproteins.
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© 1997 Birkhäuser Verlag Basel
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Weitzhandler, M., Avdalovic, N. (1997). HPAE-PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride Membranes. In: Jackson, P., Gallagher, J.T. (eds) A Laboratory Guide to Glycoconjugate Analysis. BioMethods. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7388-8_3
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DOI: https://doi.org/10.1007/978-3-0348-7388-8_3
Publisher Name: Birkhäuser Basel
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