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The effect of oxyhemoglobin on the proliferation and migration of cultured vascular advential fibroblasts

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Cerebral Vasospasm

Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 104))

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Abstract

The vascular adventitia is activated in a variety of vascular disease states but its role in cerebral vasospasm has been easily disregarded. Since oxyhemoglobin (oxyHb) is implicated as one of the most important spasmogens for cerebral vasospasm that follows aneurysmal subarachnoid hemorrhage (SAH), 1–200 μM of oxyHb were used in this study to mimic the clinical situation, and the effects of oxyHb on cells proliferation and migration of cultured rat aortic smooth muscle cells was investigated. Morphological and biochemical techniques, such as MTT assay, flow cytometry cell cycle analysis, monolayer-wounding and Boyden’s chamber migration assay were used. Results showed that low concentration of oxyHb (1–100 μM) increase the proliferation and migration of cultured VAFs. On the contrary, high concentration of oxyHb (200 μM) inhibit proliferation and migration of cultured vascular advential fibroblasts (VAFs). This study provides in vitro evidence that oxyhemoglob in could affect the proliferation and migration of cultured VAFs. The results support the hypothesis that VAFs may play a significant role in the vascular response to injury after SAH, and may be a novel potential therapeutic target in future.

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Authors and Affiliations

  1. Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, People’s Republic of China

    W. -H. Tang, G. Zhu, Z. Chen, Z. Liu & Hua Feng

  2. Division of Neurosurgery, Loma Linda University Medical Center, Loma Linda, USA

    J. H. Zhang

Authors
  1. W. -H. Tang
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  2. G. Zhu
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  3. J. H. Zhang
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  4. Z. Chen
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  5. Z. Liu
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  6. Hua Feng
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Corresponding author

Correspondence to Hua Feng .

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Editors and Affiliations

  1. Department of Neurosurgery, Medical Faculty of Istanbul, University of Istanbul, Istanbul, Turkey

    Talat Kırış

  2. Department of Neurosurgery, Loma Linda University Medical Center, Loma Linda, USA

    John H. Zhang

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© 2008 Springer-Verlag

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Tang, W.H., Zhu, G., Zhang, J.H., Chen, Z., Liu, Z., Feng, H. (2008). The effect of oxyhemoglobin on the proliferation and migration of cultured vascular advential fibroblasts. In: Kırış, T., Zhang, J.H. (eds) Cerebral Vasospasm. Acta Neurochirurgica Supplement, vol 104. Springer, Vienna. https://doi.org/10.1007/978-3-211-75718-5_37

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  • DOI: https://doi.org/10.1007/978-3-211-75718-5_37

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-75717-8

  • Online ISBN: 978-3-211-75718-5

  • eBook Packages: MedicineMedicine (R0)

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