Conclusion

Abstract

The aim of the present work was to develop a purification method for combinatorially synthesized peptide arrays. This goal was achieved by transferring the peptide array to a second solid support whereby only full-length peptides were able to re-bind via an N-terminal cysteine. In general, the method is compatible with standard micro-particle based solid phase peptide synthesis (mpSPPS) and only few additional steps are required to allow for peptide purification: The solid support was equipped with a standard acid-labile linker, the RAM linker, which could be cleaved after the peptide synthesis in the course of the side-chain deprotection using TFA. Coupling and cleavage of the RAM linker proved to be easily achieved in the peptide array synthesis, whereas the implementation of the physiologically cleavable HBA linker was not successful.