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Label-Free Impedimetric Determination of miRNA Using Biotinylated Conducting Polymer Modified Carbon Electrodes

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Sensors

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 319))

Abstract

Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.

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Acknowledgments

Miur (Prin 2012) and Università di Firenze (Fondi di Ateneo) as well as the Foundation for Polish Science (Grant MPD/2009/1/styp15 to M.S.) for funding.

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Authors and Affiliations

  1. Dipartimento di Chimica, Università degli Studi di Firenze, Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy

    D. Voccia, F. Bettazzi & I. Palchetti

  2. Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland

    M. Sosnowska & W. Kutner

Authors
  1. D. Voccia
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  2. M. Sosnowska
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  3. F. Bettazzi
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  4. I. Palchetti
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  5. W. Kutner
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Corresponding author

Correspondence to D. Voccia .

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Editors and Affiliations

  1. Faculty of Bioscience and Technologies for Food Agriculture and Environment, University of Teramo, Mosciano S.A., Teramo, Italy

    Dario Compagnone

  2. Institute of Applied Physics IFAC-CNR, Firenze, Italy

    Francesco Baldini

  3. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. University of Cassino, Cassino, Italy

    Giovanni Betta

  5. CNR, Head of Inst. of Microelectronics a, Lecce, Italy

    Pietro Siciliano

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Voccia, D., Sosnowska, M., Bettazzi, F., Palchetti, I., Kutner, W. (2015). Label-Free Impedimetric Determination of miRNA Using Biotinylated Conducting Polymer Modified Carbon Electrodes. In: Compagnone, D., Baldini, F., Di Natale, C., Betta, G., Siciliano, P. (eds) Sensors. Lecture Notes in Electrical Engineering, vol 319. Springer, Cham. https://doi.org/10.1007/978-3-319-09617-9_11

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  • DOI: https://doi.org/10.1007/978-3-319-09617-9_11

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-09616-2

  • Online ISBN: 978-3-319-09617-9

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