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Cold-Active β-Galactosidases: Sources, Biochemical Properties and Their Biotechnological Potential

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Biotechnology of Extremophiles:

Part of the book series: Grand Challenges in Biology and Biotechnology ((GCBB,volume 1))

Abstract

β-d-Galactosidases have been studied extensively in terms of their application to a variety of industrial technologies. To date, considerable research efforts have been devoted to characterization of new cold-active β-D-galactosidases which were isolated directly from selected species of bacteria and yeasts, as well as with the use of metagenomic approaches. This chapter will provide a review of current research towards cold-active β-D-galactosidases, focusing on the evaluation of the biochemical properties of this group of enzymes and their biotechnological potential in terms of their application to the enzymatic hydrolysis of lactose in milk and milk-based foodstuffs for people with lactose intolerance; the enzymatic synthesis of galactooligosaccharides and heterooligosaccharides (e.g., lactulose); the enzymatic synthesis of alkyl glycosides and nonionic surfactants, which can be used in chemical and pharmaceutical industries; and the enzymatic synthesis of functional food ingredients such as d-tagatose, which is a low-calorie, tooth-friendly sweetener that can be used in confectionery, beverages and dietary products.

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Acknowledgments

This work was supported by grant from the National Centre for Research and Development of Poland (PBS1/A9/7/2012).

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Authors and Affiliations

  1. Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, 80-233, Gdańsk, Poland

    Hubert Cieśliński, Marta Wanarska, Anna Pawlak-Szukalska, Ewelina Krajewska, Monika Wicka & Józef Kur

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  1. Hubert Cieśliński
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  2. Marta Wanarska
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Correspondence to Józef Kur .

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  1. Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Saint Barthélemy

    Pabulo H Rampelotto

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Cieśliński, H., Wanarska, M., Pawlak-Szukalska, A., Krajewska, E., Wicka, M., Kur, J. (2016). Cold-Active β-Galactosidases: Sources, Biochemical Properties and Their Biotechnological Potential. In: Rampelotto, P. (eds) Biotechnology of Extremophiles:. Grand Challenges in Biology and Biotechnology, vol 1. Springer, Cham. https://doi.org/10.1007/978-3-319-13521-2_15

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