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Partitioning and Diffusion of Fluorescently Labelled FTY720 in Resting Epithelial Cells

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Advanced Time-Correlated Single Photon Counting Applications

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 111))

Abstract

Sphingosine-1-phosphate (S1P) is a bioactive signalling molecule that mediates important cellular functions such as cell proliferation, cytoskeletal rearrangement, angiogenesis, mobilization of intracellular calcium, and immune cell trafficking. 2-Amino-2-[2-(4-octylphenyl) ethyl] propane-1,3-diol hydrochloride (also known as FTY720 or Fingolimod), a synthetic analog of sphingosine that has immunosuppressive properties, is the first oral drug approved by the U.S. FDA for treatment of multiple sclerosis. It is hypothesized that FTY720 is phosphorylated by sphingosine kinase 2 (SphK2) prior to binding to S1P receptor 1 (S1PR1) followed by internalization and degradation of the receptor. Here we examined the cellular uptake, partitioning, and diffusion of two fluorescent analogs of this drug (namely, Bodipy-FTY720) in cultured C3H10T1/2 cells, derived from mouse embryos. Multichannel imaging and co-localization with the endoplasmic reticulum (ER) tracker (Glibenclamide-Bodipy-FL) indicates that Bodipy-FTY720 resides in the ER of C3H10T1/2 cells, where it is expected to be phosphorylated by SphK2, and is excluded from the nucleus. This conclusion is supported by the rotational and translational diffusion measurements of this FTY720 analog in the ER membrane of the cells. Our results also indicate a heterogeneous environment of the cellular Bodipy-FTY720 as revealed by two-photon fluorescence lifetime imaging. These studies represent a step forward towards elucidating the effects of Bodipy moiety on the action mechanism of FTY720 at the single-cell level.

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Acknowledgments

We are grateful to Dr. Robert Bittman (Queens College, NY) for his generous gift of both fluorescent analogs that were used in these studies. D.W. acknowledges the financial support (teaching assistantship) from the Department of Chemistry and Biochemistry, Swenson College of Science and Engineering, University of Minnesota Duluth. R.T. and J.B. were supported by Summer Undergraduate Research Program (SURP) and Undergraduate Research Opportunities Program (UROP) awards. Additional financial support was provided by Grant-In-Aid of Research, Artistry and Scholarship (University of Minnesota), NSF (MCB0718741), NSF/REU, and NIH (AG030949).

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Minnesota Duluth, Duluth, MN, 55812, USA

    Dhanushka Wickramasinghe

  2. Department of Chemistry and Biochemistry, College of Pharmacy, University of Minnesota Duluth, Duluth, MN, 55812, USA

    Randi Timerman, Jillian Bartusek & Ahmed A. Heikal

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  1. Dhanushka Wickramasinghe
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Correspondence to Ahmed A. Heikal .

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  1. Becker & Hickl GmbH, Berlin, Germany

    Wolfgang Becker

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Wickramasinghe, D., Timerman, R., Bartusek, J., Heikal, A.A. (2015). Partitioning and Diffusion of Fluorescently Labelled FTY720 in Resting Epithelial Cells. In: Becker, W. (eds) Advanced Time-Correlated Single Photon Counting Applications. Springer Series in Chemical Physics, vol 111. Springer, Cham. https://doi.org/10.1007/978-3-319-14929-5_10

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