Abstract
Studies in several important areas of neuroscience, including analysis of single neurons as well as neural networks, continue to be limited by currently available experimental tools. By combining molecular probes of cellular function, such as voltage-sensitive or calcium-sensitive dyes, with advanced microscopy techniques such as multiphoton microscopy, experimental neurophysiologists have been able to partially reduce this limitation. These approaches usually provide the needed spatial resolution along with convenient optical sectioning capabilities for isolating regions of interest. However, they often fall short in providing the necessary temporal resolution, primarily due to their restrained laser scanning mechanisms. In this regard, we review a method of laser scanning for multiphoton microscopy that overcomes the temporal limitations of pervious approaches and allows for what is known as 3D Random Access Multiphoton (3D RAMP) microscopy, an imaging technique that supports full three dimensional recording of many sites of interest on physiologically relevant time scales.
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References
Bansal V, Patel S, Saggau P (2006) High-speed addressable confocal microscopy for functional imaging of cellular activity. J Biomed Opt 11:34003
Bi K, Zeng S et al (2006) Position of the prism in a dispersion-compensated acousto-optic deflector for multiphoton imaging. Appl Opt 45:8560–8565
Bullen A, Patel SS, Saggau P (1997) High-speed, random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators. Biophys J 73:477–491
Bullen A, Saggau P (1998) Indicators and optical configuration for simultaneous high-resolution recording of membrane potential and intracellular calcium using laser scanning microscopy. Pflugers Arch 436:788–796
Cotton RJ, Froudarakis E, Storer P, Saggau P, Tolias AS (2013) Three-dimensional mapping of microcircuit correlation structure. Front Neural Circ 7(151)
Escobar I, Saavedra G et al (2006) Reduction of the spherical aberration effect in high-numerical-aperture optical scanning instruments. J Opt Soc Am A Opt Image Sci Vis 23:3150–3155
Fork RL, Martinez OE, Gordon JP (1984) Negative dispersion using pairs of prisms. Opt Lett 9:150–152
Froudarakis E, Berens P, Ecker AS, Cotton RJ, Sinz FH, Yatsenko D et al (2014) Population code in mouse V1 facilitates readout of natural scenes through increased sparseness. Nat Neurosci 17(6):851–857
Gobel W, Kampa BM, Helmchen F (2007) Imaging cellular network dynamics in three dimensions using fast 3D laser scanning. Nat Methods 4:73–79
Greenberg DS, Kerr JN (2009) Automated correction of fast motion artifacts for two-photon imaging of awake animals. J Neurosci Methods 176(1):1–15
Iyer V, Hoogland TM, Saggau P (2006) Fast functional imaging of single neurons using random-access multiphoton (RAMP) microscopy. J Neurophysiol 95:535–545
Iyer V, Losavio BE, Saggau P (2003) Compensation of spatial and temporal dispersion for acousto-optic multiphoton laser-scanning microscopy. J Biomed Opt 8:460–471
Katona G, Szalay G, Maák P, Kaszás A, Veress M, Hillier D et al (2012) Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes. Nat Methods 9(2):201–208
Lechleiter JD, Lin DT, Sieneart I (2002) Multi-photon laser scanning microscopy using an acoustic optical deflector. Biophys J 83:2292–2299
Lv XH, Zhan C et al (2006) Construction of multiphoton laser scanning microscope based on dual-axis acousto-optic deflector. Rev Sci Instrum 77:046101
Mogilevtsev D, Birks TA, Russell PS (1998) Group-velocity dispersion in photonic crystal fibers. Opt Lett 23:1662–1664
Nakazawa M, Nakashima T, Kubota H (1988) Optical pulse-compression using a Teo2 acoustooptical light deflector. Opt Lett 13:120–122
Pawley J (1995) Handbook of biological confocal microscopy, 2nd edn. Plenum, New York
Reddy GD, Kelleher K et al (2008) Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity. Nat Neurosci 11:713–720
Reddy GD, Saggau P (2005) Fast three-dimensional laser scanning scheme using acousto-optic deflectors. J Biomed Opt 10:064038
Saggau P, Bullen A, Patel SS (1998) Acousto-optic random-access laser scanning microscopy: fundamentals and applications to optical recording of neuronal activity. Cell Mol Biol 44:827–846
Salome R, Kremer Y et al (2006) Ultrafast random-access scanning in two-photon microscopy using acousto-optic deflectors. J Neurosci Methods 154:161–174
Steinmeyer G (2006) Femtosecond dispersion compensation with multilayer coatings: toward the optical octave. Appl Opt 45:1484–1490
Treacy EB (1969) Optical pulse compression with diffraction gratings. IEEE J Quant Electron 5:454–458
Vucinic D, Sejnowski TJ (2007) A compact multiphoton 3D imaging system for recording fast neuronal activity. PLoS One 2, e699
Weber MJ (1986) Handbook of laser science and technology. CRC, Boca Raton, FL
Xu J, Weverka RT, Wagner KH (1996) Wide-angular-aperture acousto-optic devices. Proc SPIE 2754:104–114
Zeng S, Lv X et al (2006) Simultaneous compensation for spatial and temporal dispersion of acousto-optical deflectors for two-dimensional scanning with a single prism. Opt Lett 31:1091–1093
Acknowledgements
The development of AOD-based fast 3D random-access multi-photon microscopy is the result of the continuing efforts of many members and affiliates of the Saggau and Tolias labs. These efforts were supported by numerous grants from NIH and NSF.
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Reddy, G.D., Cotton, R.J., Tolias, A.S., Saggau, P. (2015). Random-Access Multiphoton Microscopy for Fast Three-Dimensional Imaging. In: Canepari, M., Zecevic, D., Bernus, O. (eds) Membrane Potential Imaging in the Nervous System and Heart. Advances in Experimental Medicine and Biology, vol 859. Springer, Cham. https://doi.org/10.1007/978-3-319-17641-3_18
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DOI: https://doi.org/10.1007/978-3-319-17641-3_18
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