Abstract
This paper investigates the 3D microscopic structure of ex-vivo human cardiac muscle. Usual 3D imaging techniques such as DMRI or CT do not achieve the required resolution to visualise cardio-myocytes, therefore we employ X-ray phase contrast micro-CT, developed at the European Synchrotron Radiation Facility (ESRF). Nine tissue samples from the left ventricle and septum were prepared and imaged at an isotropic resolution of 3.5 \(\upmu \)m, which is sufficient to visualise cardio-myocytes. The obtained volumes are compared with 2D histological examinations, which serve as a basis for interpreting the 3D X-ray phase-contrast results. Our experiments show that 3D X-ray phase-contrast micro-CT is a viable technique for investigating the 3D arrangement of myocytes ex-vivo at a microscopic level, allowing a better understanding of the 3D cardiac tissue architecture.
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Acknowledgements
We would like to thank F. Peyrin, C. Olivier, L. Wang and M. Ozon for technical support at ESRF Grenoble. This study was funded by the French National Research Agency (ANR) through the MOSIFAH project (Multimodal and multiscale modeling and simulation of the fibre architecture of the human heart, ANR-13-MONU-0009).
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Mirea, I. et al. (2015). Very High-Resolution Imaging of Post-Mortem Human Cardiac Tissue Using X-Ray Phase Contrast Tomography. In: van Assen, H., Bovendeerd, P., Delhaas, T. (eds) Functional Imaging and Modeling of the Heart. FIMH 2015. Lecture Notes in Computer Science(), vol 9126. Springer, Cham. https://doi.org/10.1007/978-3-319-20309-6_20
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