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Genetic Diversity, Genetic Erosion, Conservation of Genetic Resources, and Cultivation of Medicinal Plants

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Genetic Diversity and Erosion in Plants

Part of the book series: Sustainable Development and Biodiversity ((SDEB,volume 8))

Abstract

Wild or cultivated plants used in traditional and modern medicines are categorized as medicinal plants (MPs). Out of over 70,000 MPs, 3000 are traded and 900 are cultivated. Fragmentation/loss of habitats, unsustainable harvests, excessive grazing, invasive species, pollution, and climate change are destroying genetic diversity. Regular use of MPs in modern medicines, consumer/industrial merchandises, and increasing popularity of complementary and alternate (CAM) therapies are expanding national/global trade inciting irrational wild collections beyond regeneration potential of wild populations consequently losing species and genetic diversity. Investigations on endangered species indicated frightening levels of genetic erosion and dwindling population densities/sizes below minimum viable limits. Only a small fraction of known MPs have been evaluated for their genetic diversity and genetic erosion. Morphoagronomic, biochemical and molecular marker, and enzyme studies on wild and cultivated genotypes, populations, species, and geographical regions revealed genetic diversity with varied levels of polymorphism (14–100 %), number of alleles (2–14/locus), observed (0.0–1.0) and expected (0.06–0.84) heterozygosities, Nei’s gene diversity (0.12–0.36), Shannon’s index (0.08–0.51), gene flow (0.22–4.69), genetic distances (0.02–0.54), and similarities (0.02–0.98). Recovery, conservation, and cultivation programs initiated by governments have slowed down genetic erosion. Cultivation helped in relieving harvest pressure on wild flora and in preserving genetic diversity of some species. Existence of large number of species, paucity of adequate research funds, loss/degradation of forests, ever increasing local/world demand, genetic resource utilization with benefit sharing, and patent conflicts are the concerns that need to be resolved for conserving genetic diversity and preventing genetic erosion.

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References

  • Abdelloui R, Yahyaoui F, Neffati M (2014) Population structure and genetic diversity of medicinal plant species Retama raetam in southern Tunisia. Pakistan J Biol Sci 17(2):182–189

    Article  Google Scholar 

  • Aharizad S, Rahimi MH, Moghadam M, Mohebalipour N (2012) Study of genetic diversity in lemon balm (Melissa officinalis L.) populations based on morphological traits and essential oils content. Ann Biol Res 3(12):5748–5753

    Google Scholar 

  • Ahmadvandi HR, Cheghamirza K, Kahrizi D, Bahraminejad S (2013) Comparison of morpho-agronomic traits versus RAPD and ISSR markers in order to evaluate genetic diversity among Cuminum cyminum L. accessions. Australian J Crop Sci 7(3):361–367

    Google Scholar 

  • Akerele O, Heywood V, Singe H (eds) (1991) The conservation of medicinal plants. Cambridge University Press, Cambridge

    Google Scholar 

  • Alam MA, Gullati P, Gualti AK, Mishra GP, Naik PK (2009) Assessment of genetic diversity among Podophyllum hexandrum genotypes of the north-western Himalayan region for podophyllotoxin production. Indian J Biotechnol 8:391–399

    CAS  Google Scholar 

  • Ali MA, Hemaid FMA, Qurainy FA, Tarroum M, Khan S (2011) Assessment of genetic diversity among Indian populations of Cuscuta reflexa based on ITS sequences of nrDNA. J Med Plants Res 5(7):1217–1223

    CAS  Google Scholar 

  • Almeida CIM, Lin CM, Paron ME, Martins ER, Cavariani C, Tavares RC, Silva J (2012) Genetic erosion risk by environmental and antropic factors applied on strategies for Lychnophora ericoides conservation. J Med Plants Res 6(23):4024–4031

    Google Scholar 

  • Arya V, Yadav S, Yadav JP (2011) Intra-specific genetic diversity of different accessions of Cassia occidentalis by RAPD markers. Genet Eng Biotechnol J 22:1–8

    Google Scholar 

  • Atangana AR (2010) Phenotypic diversity in fruit and seed traits and neutral genetic diversity in Allanblackia floribunda. Dissertation, Laval University, Quebec

    Google Scholar 

  • Badr A, Khier ZAE, Hegazi GA, Kawi AE, Sawy AE (2012) Genetic variation in seven natural populations of Artemisia judaica L. in south Sinai using RAPD markers. World Appl Sci J 18(10):1475–1480. doi:10.5829/idosi.wasj.2012.18.10.2753

  • Balunas MJ, Kinghorn AD (2005) Drug discovery from medicinal plants. Life Sci 78(5):431–441

    Article  CAS  PubMed  Google Scholar 

  • Bansal D, Bhasin P, Yadav OP, Punia A (2012) Assessment of genetic diversity in Lepidium sativum (Chandrasur) a medicinal herb used in folklore remedies in India using RAPD. J Genet Eng Biotechnol 10:39–45

    Article  CAS  Google Scholar 

  • Barandozi FN, Naghavi MR, Enferadi ST, Mousavi A, Mostofi Y, Hassani ME (2012) Genetic diversity of accessions of Iranian Aloe vera based on horticultural traits and RAPD markers. Ind Crops Prod 37:347–351

    Article  CAS  Google Scholar 

  • Behrens J (2014) Can the utilization and the conservation of medicinal plants coexist? http://herbal-consultant.com/Julia-Behrens-Medical-Herbalist-sustainable_files/Sustainable%20Herbalism.pdf. Viewed 3 July 2014

  • Bekele E (2007) Study on actual situation of medicinal plants in Ethiopia. http://www.endashaw.com. Accessed 22 July 2014

  • Bramwell D (2003) On the size of the world’s threatened flora. Plant Talk 32:4–5

    Google Scholar 

  • Canter PH, Thomas H, Ernst E (2005) Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotechnol 23(4):180–185

    Article  CAS  PubMed  Google Scholar 

  • Chaddha KL, Gupta R (1995) Advances in horticulture, vol 11. Malhotra Publishing, New Delhi, Medicinal and aromatic plants

    Google Scholar 

  • Chattopadhyay P, Banerjee N, Chaudhary B (2012) Genetic characterization of selected medicinal Dendrobium (Orchidaceae) species using molecular markers. Res J Biol 2(4):117–125

    CAS  Google Scholar 

  • Cheng JR, Jaime A, Teixeira DS, Hua J, He L, Dai QL (2007) Research and biotechnology in sea buckthorn (Hippophae spp.). Med Arom Plant Sci Biotechnol 1(1):47–60

    Google Scholar 

  • Chirag G, Pankaj V, Naseer A, Tapan NK (2011) Molecular characterization on the nettle plant Utrica parviflora based on RAPD marker. J Pharm Biomed Sci 5(5):21–24

    Google Scholar 

  • Das S, Mandal AB (2013) RAPD analysis of Semecarpus kurzi Engler an important medicinal plant of Andaman and Nicobar. Int J Med Arom Plants 3(2):255–261

    Google Scholar 

  • de Oliveira LO, Ventturini BA, Rossi AAB, Hastenreiter SS (2010) Clonal diversity and conservation genetics of the medicinal plant Carapichea ipecacuanha (Rubiaceae). Genet Mol Biol 33(1):86–93

    Article  PubMed  PubMed Central  Google Scholar 

  • Dharmar K, Britto AJD (2011) RAPD analysis of genetic variability in wild populations of Withania somnifera (L.) Dunal. Int J Biol Technol 2(1):21–25

    Google Scholar 

  • Duke JA (1997) Phytomedicinal forest harvest in the United States. In: Bodeker G, Bhat KKS, Burley J, Vantomme P (eds) Non-wood forest products 11: Medicinal plants for forest conservation and health care, Global Initiative for Traditional Systems of Health. Oxford and Food and Agriculture Organization of the United Nations, Rome, pp 147–158

    Google Scholar 

  • Ebrahimi M, Farajpour M, Rahimmalek M (2012) Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers. Genet Mol Res 11(3):2855–2861

    Article  CAS  PubMed  Google Scholar 

  • Edwards R (2004) No remedy in sight for herbal ransacks. New Sci 181:10–11

    Google Scholar 

  • Eisenman SW (2010) Genetic and chemical variation in north American populations of the medicinal plant wild tarragon (Artemisia dracunculus L.). Dissertation, The State University of New Jersey, New Jersey

    Google Scholar 

  • Fan XX, Shen L, Zhang X, Chen XY, Fu CX (2004) Assessing genetic diversity of Ginkgo biloba L. (Ginkgoaceae) populations from China by RAPD markers. Biochem Genet 42(7–8):269–278

    Article  CAS  PubMed  Google Scholar 

  • FAO (2010) Global forest resources assessment (FRA). Main report, FAO Forest paper 163. Food and Agriculture Organization of the United Nations, Rome. http://www.fao.org/docrep/013/i1757e/i1757e.pdf

  • Farnsworth NR, Soejarto DD (1991) Global importance of medicinal plants. In: Akerele O, Heywood V, Synge H (eds) The conservation of medicinal plants. Cambridge University Press, Cambridge, pp 25–51

    Chapter  Google Scholar 

  • Fracaro F, Zacaria J, Echeverrigaray S (2005) RAPD based genetic relationships between populations of three chemotypes of Cunila galioides Benth. Biochem Syst Ecol 33:409–417

    Article  CAS  Google Scholar 

  • Fu C, Qiu Y, Kong H (2003) RAPD analysis for genetic diversity in Changium smyrnioides (Apiaceae), an endangered plant. Bot Bull Acad Sin 44:13–18

    CAS  Google Scholar 

  • Gharibi S, Rahimmalek M, Mirlohi A, Majidi MM, Sayed TBE (2011) Assessment of genetic diversity in Achillea millefolium subsp. millefolium and Achillea millefolium subsp. elbursensis using morphological and ISSR markers. J Med Plants Res 5:2413–2423

    CAS  Google Scholar 

  • Gilani SA, Fujii Y, Kikuchi A, Shinwari ZK, Watanabe KN (2011) Ecological consequences, genetic and chemical variations in fragmented populations of a medicinal plant, Justica adhatoda and implications for its conservation. Pakistan J Bot 43(special issue):29–37

    Google Scholar 

  • Göçmen B, Jermstad KD, Neale DB, Kaya Z (1996) Development of random amplified polymorphic DNA markers for genetic mapping in Pacific yew (Taxus brevifolia). Can J For Res 26:497–503

    Article  Google Scholar 

  • Goraniya S, Tusamda N, Shirolkar AR, Rao G, Murthy SN, Pawar SD (2013) Molecular analysis of Manilkara hexandra Roxb. and Averrhoa carambola L. using RAPD markers helps to understand genetic variations. Int J Pharm Pharm Sci 5(suppl 3):626–628

    Google Scholar 

  • Govarthanan M, Arunapriya S, Guruchandar A, Selvankumar T, Gnanasekaran N, Manoharan K (2014) Genetic variability among Coleus spp. studied by RAPD banding pattern analysis. http://precedings.nature.com/documents/5631/version/1/files/npre20115631-1.pdf?origin=publication_detail. Accessed 22 July 2014

  • Grünwald J, Büttel K (1996) The European phytotherapeutics market. Drugs Made Germany 39:6–11

    Google Scholar 

  • Guan P (2013) AFLP analysis of Gastrodia elata B1 from different regions. J Plant Genet Resour 14(1):71–78

    Google Scholar 

  • Guan BC, Gong X, Zhou SL (2011) Development and characterization of polymorphic microsatellite markers in Dysosma pleiantha (Berberidaceae). Am J Bot e210–e212. doi:10.3732/ajb.1100107

    Google Scholar 

  • Guo HB, Song ZP, Liang ZS, Zhang YJ (2009) Domestic cultivation may abate the contradiction between sustainable utilization and genetic diversity conservation of medicinal plants. J Med Plants Res 3(13):1184–1188

    Google Scholar 

  • György Z, Szabó M, Bacharov D, Pedryc A (2012) Genetic diversity within and among populations of roseroot (Rhodiola rosea L.) based on molecular markers. Not Bot Horti Agrobot Napoca 40(2):266–273

    Google Scholar 

  • Han J, Zhang W, Cao H, Chen S, Wang Y (2007) Genetic diversity and biogeography of the traditional Chinese medicine Gardenia jasminoides based on AFLP markers. Biochem Syst Ecol 35:138–145

    Article  CAS  Google Scholar 

  • Hegazi GA, Hanafy NAE, Ahmed SE, Elkheir ZAA, Hussein IA (2011) Genetic diversity and in vitro propagation of Salvadora persica L. Arab J Biotechnol 14(2):253–268

    Google Scholar 

  • Heywood V (1999) Medicinal and aromatic plants as global resources. Proceedings of WOCMAP-2 (2nd world congress on medicinal and aromatic plants for human welfare at Mendoza, Argentina, 1997). Biological resources sustainable use and ethnobotany. International Council for Medicinal and Aromatic Plants. http://wwwlib.teiep.gr/images/stories/acta/Acta%20500/500_1.pdf. Viewed 22 July 2014

  • Hu Y, Zhu Y, Zhang QY, Xin HL, Qin LP, Lu BR, Rahman K, Zheng HC (2008) Population genetic structure of the medicinal plant Vitex rotundifolia in China: implications for its use and conservation. J Integr Plant Biol 50(9):1118–1129. doi:10.1111/j.1744-7909.2008.00635.x

    Article  CAS  PubMed  Google Scholar 

  • Jayaram K, Prasad MNV (2008) Genetic diversity in Oroxylum indicum (L.) Vent. (Bignoniaceae), a vulnerable medicinal plant by random amplified polymorphic DNA marker. African. J Biotechnol 7(3):254–262

    CAS  Google Scholar 

  • Joy PP, Thomas J, Mathew S, Skaria BP (1998) Medicinal plants. Kerala Agricultural University, Kerala

    Google Scholar 

  • Kamenetsky R, Khassanov F, Rabinowitch HD, Auger J, Kik C (2007) Garlic biodiversity and genetic resources. Med Arom Plant Sci Biotechnol 1(1):1–5

    Google Scholar 

  • Kasagana VN, Karumuri SS (2011) Conservation of medicinal plants: Past, present and future. J Pharm Sci Res 3(8):1378–1386

    Google Scholar 

  • Kathe W (2006) Revision of the guidelines on the conservation of medicinal plants by WHO, IUCN, WWF and TRAFFIC. In: Rogers RJ, Craker LE, Lange D (eds) Medicinal and aromatic plants. Agricultural, commercial, ecological, legal, pharmacological and social aspects, Springer, Dordrecht (Wageningen UR Frontis Series 17), pp 109–120

    Google Scholar 

  • Kazemi M, Hajizadeh HS (2012) Assessment of genetic diversity of mints, Iranian wild “Mentha aquatica” populations using RAPD marker. J Agric Technol 8(1):327–336

    CAS  Google Scholar 

  • Kerry K (2009) Genetic variability in Hydrastis canadensis L. using RAPD analysis. Dissertation, University of Massachusetts, Amherst. http://scholarworks.umass.edu/theses/253. Accessed 22 July 2014

  • Khan V, Sharma S, Vinay (2008) RAPD based assessment of genetic diversity of Butea monosperma from different agroecological regions of India. Indian J Biotechnol 7:320–327

    Google Scholar 

  • Khatak S, Dhillon S, Yadav OP, Grewal A, Sheokand RN (2013) Agro-morphological and RAPD marker based characterization of genetic diversity in different genotypes of Withania somnifera L. Dunal. Int J Biotechnol Res 3(4):1–16

    Google Scholar 

  • Kim DH, Heber D, Still DW (2004) Genetic diversity of Echinacea species based upon amplified fragment length polymorphism markers. Genome 47(1):102–111

    Article  PubMed  Google Scholar 

  • Kumar PBJR, Britto AJD (2011) Population genetic differentiation of Heliotropium indicum as revealed by inter-simple sequence repeat (ISSR) analysis. J Chem Biol Phys Sci Sec B 2(1):248–253

    Google Scholar 

  • Kylin M (2010) Genetic diversity of roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands. Dissertation, Swedish University of Agricultural Sciences, Alnarp

    Google Scholar 

  • Laird SA, Pierce AR (2002) Promoting sustainable and ethical botanicals: strategies to improve commercial raw material sourcing: results from the sustainable botanicals pilot project, industry surveys, case studies and standards collection. Rainforest Alliance, New York. http://www.rainforest-alliance.org/news/2002/botanicals-strategies.pdf. Accessed 22 July 2014

  • Lal S, Mistry KN, Vaidya PB, Shah SD, Thaker RA (2011) Genetic diversity among five economically important species of Asparagus collected from central Gujarat (India) utilizing RAPD markers (random amplification of polymorphic DNA). Int J Adv Biotechnol Res 2(4):414–421

    CAS  Google Scholar 

  • Lal S, Mistry KN, Thaker R, Shah SD, Vaidya PB (2012) Genetic diversity assessment in six medicinally important species of Ocimum from central Gujarat (India) utilizing RAPD, ISSR and SSR markers. Int J Adv Biotechnol Res 2(2):279–288

    Google Scholar 

  • Lange D (2006a) What makes medicinal and aromatic plants special? Paper presented in Expert workshop on assessing the sustainable yield in MAP collection, INA, Isle of Vilm, Germany, 14–17 September. http://www.floraweb.de/map-pro/lectures/Lange.pdf. Accessed 22 July 2014

  • Lange D (2006b) International trade in medicinal and aromatic plants. In: Bogers RJ, Craker LE, Lange D (eds) Medicinal and aromatic plants. Agricultural, commercial, ecological, legal, pharmacological and social aspects, Springer, Dordrecht (Wageningen UR Frontis Series 17), pp 155–170

    Google Scholar 

  • Larsen HO (2011) Accessibility of wild products. Biodiversity for food and medicine indicators partnership. Newslet Med Plant Special Group IUCN Sp Surv Commis 14:24–29

    Google Scholar 

  • Lewington A (1993) Medicinal plant and plant extracts: A review of their importation into Europe. TRAFFIC International, Cambridge, UK

    Google Scholar 

  • Li JM, Jin ZX, Zhong ZC (2004) RAPD analysis of genetic diversity of Sargentodoxa cuneata at different altitudes and the influence of environmental factors. Acta Ecol Sin 24:567–573

    CAS  Google Scholar 

  • Li C, Chen JL, Sun Y, Wang FG, Xing FW (2012) Development of microsatellite markers for the endangered medicinal plant Launaea arborescens (Asteraceae). Am J Bot e481–e483. doi:10.3732/ajb.1200126

    Google Scholar 

  • Liu C, Yu H, Chen SL (2011) Framework for sustainable use of medicinal plants in China. Plant Divers Resour 33(1):65–68. doi:10.3724/SP.J.1143.2011.10249

    CAS  Google Scholar 

  • Liu J, Shi S, Chang E, Yang W, Jiang Z (2013) Genetic diversity of the critically endangered Thuja sutchuenensis revealed by ISSR markers and the implications for conservation. Int J Mol Sci 14:14860–14871. doi:10.3390/ijms140714860

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Lubbe A, Verpoorte R (2011) Cultivation of medicinal and aromatic plants for specialty industrial materials. Ind Crops Prod 34:785–801

    Article  CAS  Google Scholar 

  • Ma X, Xie C, Guan M, Xu X, Miki E, Takeda O, Xin T, Zheng S, Yao H, Shi L, Song J, Chen S (2014) High levels of genetic diversity within one population of Rheum tanguticum on the Qinghai-Tibet Plateau have implications for germplasm conservation. Pharm Crops 5:1–8

    Article  CAS  Google Scholar 

  • Mahesh R, Kumar NN, Sujin RM (2008) Molecular analysis in Rauvolfia tetraphylla L. using RAPD markers. Ethnobot Leaflets 12:1129–1136

    Google Scholar 

  • Matasyoh LG, Wachira FN, Kinyua MG, Muigai AWT (2011) Genetic diversity of the medicinal plant Ocimum gratissimum L. (mint) from Kenya based on AFLP markers. J Life Sci 5:91–99

    CAS  Google Scholar 

  • Mattioni C, Casasoli M, Gonzalez M, Ipinza R (2002) Comparison of ISSR and RAPD markers to characterize three Chilean Nothofagus species. Theor Appl Genet 104:1064–1070

    Article  CAS  PubMed  Google Scholar 

  • Medicinal Plant Specialist Group (2007) International standard for sustainable wild collection of medicinal and aromatic plants (ISSC-MAP), version 1.0. Bundesamt für Naturschutz (BfN), MPSG/SSC/IUCN, WWF Germany and TRAFFIC, Bonn, Gland. Frankfurt and Cambridge. BfN-Skripten 195. http://www.bfn.de/fileadmin/MDB/documents/service/skript195.pdf. Accessed 22 July 2014

  • Memdelsohn R, Balick M (1995) Drugs from rain forest. Newslet Asian Network Med Arom Plants 14:2–3

    Google Scholar 

  • Messaoud C, Afif M, Boulila A, Rejeb MN, Boussaid M (2007) Genetic variation of Tunisian Myrtus communis L. (Myrtaceae) populations assessed by isozymes and RAPD. Ann Forestry Sci 64:845–853

    Article  CAS  Google Scholar 

  • Minarchenko V (2011) Medicinal plants of Ukraine: diversity, resources, legislation. Newslet Med Plant Special Group IUCN Sp Surv Commis 14:7–13

    Google Scholar 

  • Morozowska M, Krzakowa M (2003) Genetic variation in natural and cultivated populations of Primula veris. Acta Biol Cracoviensia Ser Bot 45(2):177–182

    Google Scholar 

  • Motley TJ, Lück L, Zerega NJC (2004) Genetic diversity and DNA fingerprinting of black cohosh (Actaea racemosa). Proceedings of the global summit on medicinal plants, vol 1, pp 112–118. http://sci.odu.edu/biology/directory/Motley_files/publications/Motley_manuscript.pdf

  • Mouna HM, Reddy PJM, Rajasekharan PE, Shareef I, Sreekanth B (2014) Assessment of genetic diversity in the medicinal climber Gymnema sylvestre from Karnataka, India. Int J Innov Res Sci Eng Technol 3(3):10497–10501

    Google Scholar 

  • Muchugi A, Kindt R, Muluvi GM, Muge E, Ramni HK, Jamnadass H (2012) Genetic variation of Kenyan populations of Warburgia ugandensis, an important East African Highlands medicinal tree species. J Life Sci 4(2):97–105

    Google Scholar 

  • Nan P, Peng S, Shi S, Ren H (2003) Interpopulation congruence in Chinese Primula ovalifolia revealed by chemical and molecular markers using essential oils and ISSRs. Z Naturforsch 58:57–61

    Google Scholar 

  • Negi MS, Singh A, Lakshmikumaran M (2000) Genetic variation and relationship among and within Withania species as revealed by AFLP markers. Genome 43:975–980

    Article  CAS  PubMed  Google Scholar 

  • Nemati Z, Zeinalabedini M, Mardi M, Pirseyediand SM, Marashi SH, Nekoui SMK (2012) Isolation and characterization of a first set of polymorphic microsatellite markers in saffron, Crocus Sativus (Iridaceae). Am J Bot e340–e343. doi:10.3732/ajb.1100531

    Google Scholar 

  • Okoń S, Magdziak AS (2011) The use of RAPD markers for detecting genetic similarity and molecular identification of chamomile (Chamomilla recutita L. Rausch.) genotypes. Herba Pol 57(1):38–47

    Google Scholar 

  • Padmalatha K, Prasad MNV (2007) Inter and intra population genetic variation of Rauvolfia serpentina (L.) Benth. ex Kurz. an endangered medicinal plant by RAPD analysis. Med Arom Plant Sci Biotechnol 1(1):118–123

    Google Scholar 

  • Palevitch D (1991) Agronomy applied to medicinal plants conservation. In: Akerele O, Heywood V, Singe H (eds) The conservation of medicinal plants. Cambridge University Press, Cambridge, pp 168–178

    Google Scholar 

  • Paliwal R, Singh R, Singh AK, Kumar S, Kumar A, Majumdar RS (2013) Molecular characterization of giloe (Tinospora cordifolia Willd. Miers ex Hook. f. & Thoms.) accession using start codon targeted (SCoT) markers. Int J Med Arom Plants 3(4):413–422

    Google Scholar 

  • Pandey M, Changtragoon S (2012) Isolation and characterization of microsatellites in a medicinal plant Phyllanthus emblica (Euphorbiaceae). Am J Bot e468–e469. doi:10.3732/ajb.1200157

    Google Scholar 

  • Pang YX, Wang WQ, Zhang YB, Yuan Y, Yu JB, Zhu M, Chen YY (2014) Genetic diversity of the Chinese traditional herb Blumea balsamifera (Asteraceae) based on AFLP markers. Genet Mol Res 13(2):2718–2726

    Article  CAS  PubMed  Google Scholar 

  • Paton A (2009) Biodiversity informatics and the plant conservation baseline. Trends Plant Sci 14(11):629–637. doi:10.1016/j.tplants.2009.08.007

    Article  CAS  PubMed  Google Scholar 

  • Pezhmanmehr M, Hassani ME, Jahansooz F, Najafi AA, Sefidkon F, Mardi M, Pirseiedi M (2009) Assessment of genetic diversity in some Iranian populations of Bunium persicum using RAPD and AFLP markers. Iranian J Biotechnol 7(2):93–100

    CAS  Google Scholar 

  • Pilepic KH, Males Z, Plazibat M (2008) Genetic structure in Hypericum perforatum L. population. Periodicum Biologorum 110(4):367–371

    Google Scholar 

  • Pop MR, Sand C, Barbu CH (2008) Genetic distance determination in some genotypes of Arnica montana L. by RAPD technique. Bull UASVM Agric 65(1):201–203

    Google Scholar 

  • Principe PP (1991) Valuing biodiversity of medicinal plants. In: Akerele O, Heywood V, Singe H (eds) The conservation of medicinal plants. Cambridge University Press, Cambridge, pp 53–63

    Google Scholar 

  • Qing JY, Zhi YZ, Juan H, Lan PG, Ai JS, Lu QH (2010) Impacts of recent cultivation on genetic diversity pattern of a medicinal plant Scutellaria baicalensis (Lamiaceae). BMC Genet 11(29):1–13. doi:10.1186/1471-2156-11-29

    Google Scholar 

  • Rahimmalek M (2012) Genetic relationships among Achillea tenuifolia accessions using molecular and morphological markers. Plant Omics J 5(2):128–135

    Google Scholar 

  • Rajeswara Rao BR (1999) Medicinal plants for dry areas. In: Singh RP, Osman M (eds) Sustainable alternate land use systems for drylands. Oriental Enterprises, Dehra Dun, pp 139–156

    Google Scholar 

  • Rajeswara Rao BR (2012) Cultivation, economics and marketing of Phyllanthus species. In: Kuttan R, Harikumar KB (eds) Phyllanthus species: scientific evaluation and medicinal applications. CRC Press, Boca Raton, pp 47–70

    Google Scholar 

  • Rajeswara Rao BR, Rajput DK (2005) Organic farming: medicinal and aromatic crops. Proceedings of national seminar on organic farming- current scenario and future thrust. Acharya NG Ranga Agricultural University, Hyderabad, 27–28 Apr 2005, pp 41–51

    Google Scholar 

  • Rajeswara Rao BR, Singh K, Sastry KP, Singh CP, Kothari SK, Rajput DK, Bhattacharya AK (2007) Cultivation technology for economically important medicinal plants. In: Janardhan Reddy K, Bahadur B, Bhadraiah B, Rao MLN (eds) Advances in medicinal plants, Universities Press India Pvt Ltd, Hyderabad, pp 112–122

    Google Scholar 

  • Rajeswara Rao BR, Syamasundar KV, Rajput DK, Nagaraju G, Adinarayana G (2012) Biodiversity, conservation and cultivation of medicinal plants. J Pharmacogn 3(2):59–62

    Google Scholar 

  • Ran MX (2008) Status quo and development suggestions for cultivation of medicinal plants in China. Modern Chin Med 10(3):3–6

    Google Scholar 

  • Rao MR, Rajeswara Rao BR (2006) Medicinal plants in tropical homegardens. In: Kumar BM, Nair PKR (eds) Tropical homegardens: a time tested example of agroforestry. Springer, Dordrecht, pp 205–232

    Chapter  Google Scholar 

  • Rawashdeh IM (2011a) Genetic diversity analysis of Achillea fragrantissima (Forskal) Schultz Bip populations collected from different regions of Jordan using RAPD markers. Jordan J Biol Sci 4(1):21–28

    Google Scholar 

  • Rawashdeh IM (2011b) Genetic variability in a medicinal plant Artemisia judaica using random amplified polymorphic DNA (RAPD) markers. Int J Agric Biol 13:279–282

    Google Scholar 

  • Reddy KJ, Rajeswara Rao BR (2006) Frontiers of medicinal plant research: Biotechnology and biodiversity. Proceedings of Agribiotech 2006: first international conference on biotechnology for sustainable agriculture and agroindustry. Andhra Pradesh Industries Development Corporation, Hyderabad, pp 134–139

    Google Scholar 

  • Robbins CS (1998) American ginseng: The root of North America’s medicinal herb trade. TRAFFIC, Washington

    Google Scholar 

  • Rodrigues L, van den Berg C, Póvoa O, Monteiro A (2013) Low genetic diversity and significant structuring in the endangered Mentha cervina populations and its implications for conservation. Biochem Syst Ecol 50:51–61

    Article  CAS  Google Scholar 

  • Saidi M, Movahedi K, Mehrabi AA (2013) Characterization of genetic diversity in Satureja bachtiarica germplasm in Ilam proviance (Iran) using ISSR and RAPD markers. Intl J Agric Crop Sci 5(17):1934–1940

    Google Scholar 

  • Saini S, Yadav JP (2013) Genetic variation in natural populations of Salvadora oleoides: an important medicinal plant that needs conservation. Asian J Plant Sci Res 3(5):20–27

    Google Scholar 

  • Sairkar P, Vijay N, Batav N, Silawat N, Garg RK, Chouhan S, Sharma R, Mehrotra NN (2013) Genetic variability in two economically important medicinal plants of Madhya Pradesh, India Withania somnifera and Rauwolfia serpentina using RAPD markers. Asian J Exp Biol Sci 4(1):36–43

    CAS  Google Scholar 

  • Sanders JMC, Hamrick JL (2004) Genetic diversity in harvested and protected populations of wild American ginseng, Panax quinquefolius L. (Araliaceae). Am J Bot 91(4):540–548

    Article  Google Scholar 

  • Sanders JMC, Hamrick JL, Ahumada JA (2005) Consequences of harvesting for genetic diversity in American ginseng (Panax quinquefolius L.): a simulation study. Biodivers Conserv 14:493–504

    Article  Google Scholar 

  • Sangwan RS, Sangwan NS, Jain DC, Kumar S, Ranade AS (1999) RAPD profile based genetic characterization of chemotypic variants of Artemisia annua L. Biochem Mol Biol Int 47:935–944

    CAS  PubMed  Google Scholar 

  • Sarkhail P, Nikan M, Sarkheil P, Gohari AR, Ajani Y, Hosseini R, Hadjiakhoondi A, Saeidnia S (2014) Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships. DARU J Pharm Sci 22(32):1–9

    Google Scholar 

  • Sathyanarayana N, Leelambika M, Mahesh S, Jaheer M (2011) AFLP assessment of genetic diversity among Indian Mucuna accessions. Physiol Mol Biol Plants 17(2):171–180

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schippmann U, Leaman D, Cunnigham AB (2006) A comparison of cultivation and wild collection of medicinal and aromatic plants under sustainable aspects. In: Rogers RJ, Craker LE, Lange D (eds) Medicinal and aromatic plants. Agricultural, commercial, ecological, legal, pharmacological and social aspects. Springer, Dordrecht, pp 75–95 (Wageningen UR Frontis Series 17)

    Google Scholar 

  • Secretariat of the Convention on Biological Diversity (2002) Bonn guidelines on access to genetic resources and fair and equitable sharing of the benefits arising out of their utilization, Montreal. https://www.cbd.int/doc/publications/cbd-bonn-gdls-en.pdf. Accessed 22 July 2014

  • Seyed FM, Mohammad RH, Mohammad EH, Fatemeh S (2012) Evaluation of genetic diversity among some wild populations of Achillea bieberstenii Afan. from Iran using morphological and agronomical traits. Int J For Soil Eros 2(1):8–17

    Google Scholar 

  • Shaanker RU, Ganeshaiah KN (1997) Mapping genetic diversity of Phyllanthus emblica: forest gene banks as a new approach for in situ conservation of genetic resources. Curr Sci 73(2):163–168

    Google Scholar 

  • Sharma S, Rathi N, Kamal B, Pundir D, Kaur B, Arya S (2010) Conservation of biodiversity of highly important medicinal plants of India through tissue culture technology- a review. Agric Biol J North Am 1(5):827–833

    Article  Google Scholar 

  • Shasany AK, Darokar MP, Sakia D, Rajkumar S, Sundaresan V, Khanuja SPS (2003) Genetic diversity and species relationship in Asparagus spp. using RAPD analysis. J Med Arom Plant Sci 25:698–704

    CAS  Google Scholar 

  • Shaw RK, Acharya L, Mukherjee AK (2009) Assessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers. Crop Breed Appl Biotechnol 9:52–59

    Article  Google Scholar 

  • Sheng Y, Zheng WH, Pei KQ, Ma KP (2004) Population genetic structure of a dominant desert tree, Haloxylon ammodendron (Chenopodiaceae), in the southeast Gurbantunggut desert detected by RAPD and ISSR markers. Acta Bot Sin 46(6):675–681

    CAS  Google Scholar 

  • Shi W, Yang CF, Chen JM, Guo YH (2008) Genetic variation among wild and cultivated populations of the Chinese medicinal plant Coptis chinensis (Ranunculaceae). Plant Biol 10:485–491

    Article  CAS  PubMed  Google Scholar 

  • Shreshta JKC, Bhattari T, Sijapati J, Rana N, Maharjan J, Rawal DS, Raskoti BB, Shreshta S (2013) Assessment of genetic diversity in Napalese populations of Swertia chirayita (Roxb. ex Fleming) H. Karst using RAPD-PCR technique. Am J Plant Sci 4:1617–1628

    Article  CAS  Google Scholar 

  • Singh DR, Srivastava AK, Srivastava A, Srivastava RC (2011) Genetic diversity among three Morinda species using RAPD and ISSR markers. Indian J Biotechnol 10:285–293

    CAS  Google Scholar 

  • Singh B, Uniyal AK, Rawat JSM, Rana DK (2012) Estimation of genetic variability in Phyllanthus emblica L.: towards a contribution in sustainable rural development. J Hort Forestry 45:92–95

    Google Scholar 

  • Sonboli A, Gholipour A, Mirjalili MH, Rad MA (2011) Molecular characterization of Iranian Dracocephalum (Lamiaceae) species based on RAPD data. Acta Biol Szegediensis 55(2):227–230

    Google Scholar 

  • Soni K, Shruti R, Ankit G, Karma Y, Saurabh P, Pradeep KN, Singh H (2010) Genetic characterization of Rhodiola rosea using gene specific SSR and CAPS molecular markers. Genet Eng Biotechnol J 11:1–10

    Google Scholar 

  • Srivastava J, Lambert J, Vietmeyer N (1996) Medicinal plants: An expanding role in development. World Bank technical paper 320, The World Bank, Washington DC

    Google Scholar 

  • Srivastava V, Khan AS, Banerjee S (2009) An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage. Plant Cell Tiss Organ Cult. doi:10.1007/s11240-009-9593-z

    Google Scholar 

  • Stewart CN, Excoffier L (1996) Assessing population genetic structure and variability with RAPD data: application to Vaccinium macrocarpon. J Evol Biol 9:153–171

    Article  CAS  Google Scholar 

  • Still DW, Kim DH, Aoyama N (2005) Genetic variation in Echinacea angustifolia along a climatic gradient. Ann Bot 96(3):467–477

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Su YJ, Wang T, Huang C, Zhu JM, Zhou Q (1999) RAPD analysis of different population of Dacydium pierrei. Acta Sci Nat Univ Sunyatseni 38:99–101

    Google Scholar 

  • Sultan P, Shawl AS, Rehman S, Ahmed SF, Ramteke PW (2010) Molecular characterization and marker based chemotaxonomic studies of Podophyllum hexandrum Royle. Fitoterapia 81:243–247

    Article  CAS  PubMed  Google Scholar 

  • Suthar S, Thul S, Kukreja AK, Ramawat KG (2008) RAPD markers reveal polymorphism in Commiphora wightii, an endangered medicinal tree. J Cell Tissue Res 8(2):1477–1480

    CAS  Google Scholar 

  • Tariq A, Tariq M, Ahmad M, Sahar U, Mushtaq M, Zafar M (2014) Comparative assessment of genetic diversity among the Asclepiadaceous species using randomly amplified polymorphic DNA (RAPD) markers and numerical taxonomy system (NTSYS) cluster analysis. J Med Plant Res 8(2):88–94. doi.10.5897/JMPR09.334

  • Tripathi N, Saini N, Mehto V, Kumar S, Tiwari S (2012) Assessment of genetic diversity among Withania somnifera collected from central India using RAPD and ISSR analysis. Med Arom Plant Sci Biotechnol 6(1):33–39

    Google Scholar 

  • Ved DK, Goraya GS (2008) Demand and supply of medicinal plants in India. FRLHT, Bangalore and National Medicinal Plants Board, New Delhi

    Google Scholar 

  • Veteläinen M, Helgadóttir Á, Weibull J (2007) Climatic change and genetic resources in northern Europe. Report of a Workshop, Rovaniemi, Finland, Bioversity International, Rome, Italy, 18–19 Sept 2006

    Google Scholar 

  • Vyas GK, Kumar V, Sharma R, Sharma RA, Singh JP, Kumar S (2012) Chemical and genetic diversity among some wild stands of Calligonum polygonoides (Polygonaceae) from Thar desert of Rajasthan. Int J Trop Biol 60(3):1097–1108

    Google Scholar 

  • Walter KS, Gillett HJ (1998) 1997 IUCN red list of threatened plants. IUCN, Gland

    Google Scholar 

  • Wang XM, Hou XQ, Zhang YQ, Yang R, Feng SF, Li Y, Ren Y (2012) Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by inter-simple sequence repeat (ISSR) markers. Int J Mol Sci 13:3900–3915

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • WHO (2003) WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants. World Health Organization, Geneva

    Google Scholar 

  • WHO, IUCN, WWF (1993) Guidelines on the conservation of medicinal plants. IUCN, Gland. http://apps.who.int/medicinedocs/documents/s7150e/s7150e.pdf

  • Xu Y, Li Z, Wang Y, Huang H (2007) Allozyme diversity and population genetic structure of three medicinal Epimedium species from Hubei. J Genet Genom 34(1):56–71

    Article  CAS  Google Scholar 

  • Yan H, Anru L (2011) Population genetic diversity and structure of a naturally isolated plant species Rhodiola dumulosa (Crassulaceae). PLoS ONE 6(9):e24497. doi:10.1371/journal.pone.0024497

    Article  CAS  Google Scholar 

  • You YF, Deng HP (2012) Analysis of genetic diversity of the rare and endangered species Cibotium barometz by SRAP markers. Acta Bot Bor Occid Sin 32(4):688–692

    CAS  Google Scholar 

  • Zeng S, Xiao G, Guo J, Fei Z, Xu Y, Roe BA, Wang Y (2010) Development of a EST dataset and characterization of EST-SSRs in traditional Chinese medicinal plant, Epimedium sagittatum (Sieb. et Zucc.) Maxim. BMC Genet 11(94):1–11

    Google Scholar 

  • Zhang DQ, Gao LM, Yang YP (2010) Genetic diversity and structure of a traditional Chinese medicinal plant species Fritillaria cirrhosa (Liliaceae) in southwest China and implications for its conservation. Biochem Syst Ecol 38:236–242

    Article  CAS  Google Scholar 

  • Zhang L, Zhao HX, Fan X, Wang M, Ding CB, Yang RW (2012) Genetic diversity among Salvia miltiorrhiza Bunge and related species inferred from nrDNA ITS sequences. Turk J Biol 36:319–326. doi:10.3906/biy-1104-12

    Google Scholar 

  • Zhao KG, Zhou MQ, Chen LQ (2007) Genetic diversity and discrimination of Chimonanthus praecox (L.) link germplasm using ISSR and RAPD markers. Hortsci 42(5):1144–1148

    CAS  Google Scholar 

  • Zheng JY, Wang H, Chen XX, Wang P, Gao P, Li XN, Zhu GP (2012) Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae). Genet Mol Res 11(3):1975–1980

    Article  CAS  PubMed  Google Scholar 

  • Zhou L, Wan Y, Zhang L (2008a) Genetic diversity and relationship of Rhododendron Species based on RAPD analysis. Am Euras J Agric Environ Sci 3(4):626–631

    Google Scholar 

  • Zhou Y, Zhou C, Yao H, Liu Y, Tu R (2008b) Application of ISSR markers in detection of genetic variation among Chinese yam (Dioscorea opposita Thunb) cultivars. Life Sci J 5(4):6–12

    CAS  Google Scholar 

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Acknowledgment

The author is beholden to the Director, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, for facilities and encouragement.

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  1. CSIR-Central Institute of Medicinal and Aromatic Plants Research Center, Boduppal, Hyderabad, 500092, India

    B. R. Rajeswara Rao

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  1. Zobel Forestry Associates, New Paltz, New York, USA

    M.R. Ahuja

  2. University of Helsinki, Helsinki, Finland

    S. Mohan Jain

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Rajeswara Rao, B.R. (2016). Genetic Diversity, Genetic Erosion, Conservation of Genetic Resources, and Cultivation of Medicinal Plants. In: Ahuja, M., Jain, S. (eds) Genetic Diversity and Erosion in Plants. Sustainable Development and Biodiversity, vol 8. Springer, Cham. https://doi.org/10.1007/978-3-319-25954-3_11

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