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Improvement of Industrially Relevant Biological Activities in Mucoromycotina Fungi

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Gene Expression Systems in Fungi: Advancements and Applications

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Mucoromycotina contain several biotechnologically important fungi, primarily in the orders Mucorales and Mortierellales, which are considered as excellent producers of lipases and proteases, organic acids, alcohol, carotenoids and polyunsaturated fatty acids. Here, we overview gene expression and metabolic engineering studies performed to improve industrially relevant activities in different Mucoromycotina fungi in the past decade. Genetic transformation methods used to manipulate these fungi, especially Rhizopus oryzae, Mucor circinelloides and Mortierella alpina, and promoters used successfully to express genes in them will be reviewed. Concerning the improvement of production of hydrolytic enzymes special attention has been paid to the heterologous expression of lipases, which are among the best characterized enzymes in these fungi. Main achievements in the improvement of lactic acid and fumaric acid production by Rhizopus enzymes have also been summarized. Expression of endogenous and exogenous terpenoid and carotenoid biosynthetic genes to enhance and modify the carotene production of Mucor circinelloides has been detailed. Finally, homologous and heterologous expression of Mucoromycotina fatty acid desaturases and elongases in fungi and plants has been overviewed with special attention to the production of arachidonic acid and γ-linolenic acid.

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Acknowledgements

This work was supported by the grants OTKA NN106394 and the Hungarian-Slovak TÉT_12_SK-1-2013-0007. MT is a grantee of postdoctoral grant OTKA PD 112234; ÁC and TP are grantees of the Postdoctoral Research Programme (Postdoc-16, 45.0.080) and the J. Bolyai Research Scholarship of the Hungarian Academy of Sciences, respectively.

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  1. Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., Szeged, 6726, Hungary

    Tamás Papp Ph.D., Ildikó Nyilasi Ph.D., Árpád Csernetics Ph.D., Gábor Nagy Ph.D., Miklós Takó Ph.D. & Csaba Vágvölgyi D.Sc., Ph.D.

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  1. Tamás Papp Ph.D.
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  2. Ildikó Nyilasi Ph.D.
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  3. Árpád Csernetics Ph.D.
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  4. Gábor Nagy Ph.D.
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  5. Miklós Takó Ph.D.
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  6. Csaba Vágvölgyi D.Sc., Ph.D.
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Correspondence to Tamás Papp Ph.D. .

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  1. and Environment-Bioresources, AIT Austrian Institute of Technology Department of Health, Tulln, Australia

    Monika Schmoll

  2. and Environment-Bioresources, AIT Austrian Institute of Technology Department of Health, Tulln, Australia

    Christoph Dattenböck

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Papp, T., Nyilasi, I., Csernetics, Á., Nagy, G., Takó, M., Vágvölgyi, C. (2016). Improvement of Industrially Relevant Biological Activities in Mucoromycotina Fungi. In: Schmoll, M., Dattenböck, C. (eds) Gene Expression Systems in Fungi: Advancements and Applications. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-27951-0_4

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